Synaptic Systems

Cat. No.: 362 003

Amount: 50 µg

Price: $380.00

Cat. No. 362 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 68 from mouse Zbtb20 (UniProt Id: Q8K0L9)
Reactivity	Reacts with: rat (D4A1U1), mouse (Q8K0L9). Other species not tested yet.
Data sheet	362_003.pdf

References for Zbtb20 - 362 003

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 362 003 IHC; tested species: mouse

Brain erythropoietin fine-tunes a counterbalance between neurodifferentiation and microglia in the adult hippocampus.
Fernandez Garcia-Agudo L, Steixner-Kumar AA, Curto Y, Barnkothe N, Hassouna I, Jähne S, Butt UJ, Grewe K, Weber MS, Green K, Rizzoli S, et al.
Cell reports (2021) 368: 109548. 362 003 IHC; tested species: mouse

Cat. No.: 362 003

Amount: 50 µg

Price: $380.00

ICC

IHC

Background

The zinc finger and broad complex, tramtrack, bric-a-brac domain-containing protein 20 or Zbtb 20 is a transcription factor expressed by developing hippocampal projection neurons. The hippocampus of Zbtb 20 K.O. mice was reduced in size, and exhibited increased apoptotic cell death during postnatal development.
In mature neurons Zbtb 20 is involved in the regulation of hippocampus dependent memory.

1 ) Nielsen JV et al., Cereb. Cortex (2014)
2 ) Ren A et al., J. Physiol. (Lond.) (2012)
3 ) Xie Z et al., Proc. Natl. Acad. Sci. U.S.A. (2010)
4 ) Mitchelmore C et al., J. Biol. Chem. (2002)

Protocols

ICC

IHC

Zbtb20 antibody - 362 003

References for Zbtb20 - 362 003