YidC antibody - 383 003

YidC is a prokaryotic protein insertase

Rabbit polyclonal purified antibody

Cat. No.: 383 003

Amount: 50 µg

Price: $375.00

Cat. No. 383 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 260 to 367 from YidC (UniProt Id: A0R7J7)
Reactivity	Reacts with: prokaryotes. Other species not tested yet.
Data sheet	383_003.pdf

Cat. No.: 383 003

Amount: 50 µg

Price: $375.00

Background

The insertion of proteins into the prokaryotic plasma membrane is catalyzed by translocases and insertases. YidC insertases interact with their substrates at an amphiphilic protein-lipid interface mediating the insertion of the substrate's transmembrane segment into the lipid bilayer.

1 ) Kuhn A et al., Mol. Microbiol. (2017)
2 ) Serdiuk T et al., Nat. Chem. Biol. (2016)
3 ) Thakur P et al., Sci Rep (2016)

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