Synaptic Systems

Cat. No.: 324 103

Amount: 50 µg

Price: $375.00

Cat. No. 324 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 30 to 99 from human WRB (UniProt Id: O00258)
Reactivity	Reacts with: rat (Q6P6S5), mouse (Q8K0D7), human (O00258). Other species not tested yet.
Specificity	K.D. validated PubMed: 27226539
Data sheet	324_103.pdf

References for WRB - 324 103

ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence.
Hirata T, Yang J, Tomida S, Tokoro Y, Kinoshita T, Fujita M, Kizuka Y
The Journal of biological chemistry (2022) : 102444. 324 103 WB; tested species: human

The WRB Subunit of the Get3 Receptor is Required for the Correct Integration of its Partner CAML into the ER.
Carvalho HJF, Del Bondio A, Maltecca F, Colombo SF, Borgese N
Scientific reports (2019) 91: 11887. 324 103 WB, IP; tested species: human

Discrimination between the endoplasmic reticulum and mitochondria by spontaneously inserting tail-anchored proteins.
Figueiredo Costa B, Cassella P, Colombo SF, Borgese N
Traffic (Copenhagen, Denmark) (2018) 193: 182-197. 324 103 WB

Tail-anchored Protein Insertion in Mammals: FUNCTION AND RECIPROCAL INTERACTIONS OF THE TWO SUBUNITS OF THE TRC40 RECEPTOR.
Colombo SF, Cardani S, Maroli A, Vitiello A, Soffientini P, Crespi A, Bram RF, Benfante R, Borgese N
The Journal of biological chemistry (2016) 29129: 15292-306. 324 103 WB; KD verified; tested species: rat

Cat. No.: 324 103

Amount: 50 µg

Price: $375.00

Background

The widely expressed tryptophan (W)-rich basic protein (WRB), also known as congenital heart disease protein 5 (CHD 5) has initially been described as a nuclear protein that plays a potential role in the pathogenesis of CHD in down syndrome patients.
Recently this protein has been identified as the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane.

1 ) Vilardi F et al., PLoS ONE (2014)
2 ) Vilardi F et al., J. Cell. Sci. (2011)
3 ) Egeo A et al., Hum. Genet. (1998)

Protocols

Standard protocol

Other products for this target

WRB antibody - 324 103 K.D.

References for WRB - 324 103