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Home > Products > 137 002

VPS45 antibody - 137 002

VPS45 is a member of the SM family, probably involved in trans-Golgi network trafficking
Rabbit polyclonal antiserum
Cat. No.: 137 002
Amount: 200 µl
Price: $355.00
Cat. No. 137 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 570 from human VPS45 (UniProt Id: Q9NRW7)
Reactivity Reacts with: human (Q9NRW7), rat (O08700), mouse (P97390).
Other species not tested yet.
Data sheet 137_002.pdf

References for VPS45 - 137 002

Sorting of GLUT4 into its insulin-sensitive store requires the Sec1/Munc18 protein mVps45.
Roccisana J, Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2013) 2415: 2389-97. 137 002 WB
The Thr224Asn mutation in the VPS45 gene is associated with the congenital neutropenia and primary myelofibrosis of infancy.
Stepensky P, Saada A, Cowan M, Tabib A, Fischer U, Berkun Y, Saleh H, Simanovsky N, Kogot-Levin A, Weintraub M, Ganaiem H, et al.
Blood (2013) 12125: 5078-87. 137 002 WB; tested species: human
Detergent-free isolation and characterization of cholesterol-rich membrane domains from trans-Golgi network vesicles.
Waugh MG, Chu KM, Clayton EL, Minogue S, Hsuan JJ
Journal of lipid research (2011) 523: 582-9. 137 002 WB
Common and distinct roles for the binding partners Rabenosyn-5 and Vps45 in the regulation of endocytic trafficking in mammalian cells.
Rahajeng J, Caplan S, Naslavsky N
Experimental cell research (2010) 3165: 859-74. 137 002 WB
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 137 002 WB
Characterization of the role of the Rab GTPase-activating protein AS160 in insulin-regulated GLUT4 trafficking.
Larance M, Ramm G, Stöckli J, van Dam EM, Winata S, Wasinger V, Simpson F, Graham M, Junutula JR, Guilhaus M, James DE, et al.
The Journal of biological chemistry (2005) 28045: 37803-13. 137 002 WB
Cat. No.: 137 002
Amount: 200 µl
Price: $355.00
Sorting of GLUT4 into its insulin-sensitive store requires the Sec1/Munc18 protein mVps45.
Roccisana J, Sadler JB, Bryant NJ, Gould GW
Molecular biology of the cell (2013) 2415: 2389-97. 137 002 WB
The Thr224Asn mutation in the VPS45 gene is associated with the congenital neutropenia and primary myelofibrosis of infancy.
Stepensky P, Saada A, Cowan M, Tabib A, Fischer U, Berkun Y, Saleh H, Simanovsky N, Kogot-Levin A, Weintraub M, Ganaiem H, et al.
Blood (2013) 12125: 5078-87. 137 002 WB; tested species: human
Detergent-free isolation and characterization of cholesterol-rich membrane domains from trans-Golgi network vesicles.
Waugh MG, Chu KM, Clayton EL, Minogue S, Hsuan JJ
Journal of lipid research (2011) 523: 582-9. 137 002 WB
Common and distinct roles for the binding partners Rabenosyn-5 and Vps45 in the regulation of endocytic trafficking in mammalian cells.
Rahajeng J, Caplan S, Naslavsky N
Experimental cell research (2010) 3165: 859-74. 137 002 WB
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 137 002 WB
Characterization of the role of the Rab GTPase-activating protein AS160 in insulin-regulated GLUT4 trafficking.
Larance M, Ramm G, Stöckli J, van Dam EM, Winata S, Wasinger V, Simpson F, Graham M, Junutula JR, Guilhaus M, James DE, et al.
The Journal of biological chemistry (2005) 28045: 37803-13. 137 002 WB
WB
Background
The vesicular transport protein VPS 45 has first been discovered in yeast, and homologues were later found in mammals. This ubiquitiously expressed protein belongs to the STxBP/Unc-18/Sec1 family. It is probably involved in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. The highest expression levels are observed in brain and testis.