Vimentin antibody - 172 003 K.O.

Vimentin is a type III intermediate filament

Rabbit polyclonal purified antibody

Cat. No.: 172 003

Amount: 50 µg

Price: $455.00

Cat. No. 172 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 466 from mouse Vimentin (UniProt Id: P20152)
Reactivity	Reacts with: human (P08670), rat (P31000), mouse (P20152), ape. No signal: zebrafish. Other species not tested yet.
Specificity	K.O. validated
Data sheet	172_003.pdf

Cat. No.: 172 003

Amount: 50 µg

Price: $455.00

ICC

IHC

Background

Vimentin belongs to the family of intermediate filaments that can be subdivided into six major groups based on sequence similarity. Vimentin belongs to the type III category and is the predominant subunit protein of intermediate filaments in tissues of mesenchymal origin.
Like other intermediate filaments it plays a role in the cytoskeletal organization and maintenance of cell shape and morphology.

1 ) Styers ML et al., J. Cell. Sci. (2006)
2 ) Esue O et al., J. Biol. Chem. (2006)
3 ) Miyaguchi K et al., J. Struct. Biol. (1997)
4 ) Chiu FC et al., J. Neurochem. (1981)
5 ) Browning ET et al., J. Cell Biol. (1981)
6 ) Gard DL et al., Cell (1980)

Protocols

ICC

IHC

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