Synaptic Systems

Cat. No.: 135 511

Amount: 100 µg

Price: $420.00

Cat. No. 135 511	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 100 up to 1 : 1000 (AP staining) (see remarks) IP: yes ICC: 1 : 100 up to 1 : 500 gallery IHC: 1 : 100 gallery IHC-P: 1 : 100 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	317G6
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat VGLUT 1 (UniProt Id: Q62634)
Epitop	AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity	Reacts with: rat (Q62634), mouse (Q3TXX4). Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	135-3P
Remarks	WB: This antibody yields weaker signals in Western blot experiments than 135 311, 135 011 and the polyclonal antibodies 135 302, 135 303, 135 304 and 135 306. VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	135_511.pdf

References for VGLUT1 - 135 511

A Neuron, Microglia, and Astrocyte Triple Co-culture Model to Study Alzheimer's Disease.
Luchena C, Zuazo-Ibarra J, Valero J, Matute C, Alberdi E, Capetillo-Zarate E
Frontiers in aging neuroscience (2022) 14: 844534. 135 511 WB; tested species: rat

Caspr2 interacts with type 1 inositol 1,4,5-trisphosphate receptor in the developing cerebellum and regulates Purkinje cell morphology.
Argent L, Winter F, Prickett I, Carrasquero-Ordaz M, Olsen AL, Kramer H, Lancaster E, Becker EBE
The Journal of biological chemistry (2020) 29536: 12716-12726. 135 511 WB; tested species: mouse

Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development.
Bikbaev A, Ciuraszkiewicz-Wojciech A, Heck J, Klatt O, Freund R, Mitlöhner J, Enrile Lacalle S, Sun M, Repetto D, Frischknecht R, Ablinger C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4025: 4824-4841. 135 511 WB; tested species: mouse

APC/CCdh1-Rock2 pathway controls dendritic integrity and memory.
Bobo-Jiménez V, Delgado-Esteban M, Angibaud J, Sánchez-Morán I, de la Fuente A, Yajeya J, Nägerl UV, Castillo J, Bolaños JP, Almeida A
Proceedings of the National Academy of Sciences of the United States of America (2017) 11417: 4513-4518. 135 511 WB

Distribution of SNAP25, VAMP1 and VAMP2 in mature and developing deep cerebellar nuclei after estrogen administration.
Manca P, Mameli O, Caria MA, Torrejón-Escribano B, Blasi J
Neuroscience (2014) 266: 102-15. 135 511 IHC, WB

Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses.
Zander JF, Münster-Wandowski A, Brunk I, Pahner I, Gómez-Lira G, Heinemann U, Gutiérrez R, Laube G, Ahnert-Hilger G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3022: 7634-45. 135 511 IP

Persistence of quantal synaptic vesicle recycling in virtual absence of dynamins.
Afuwape OAT, Chanaday NL, Kasap M, Monteggia LM, Kavalali ET
The Journal of physiology (2024) : . 135 511 ICC; tested species: mouse

Dissection of signaling pathways regulating TrkB-dependent gephyrin clustering.
Wüstner LS, Beuter S, Kriebel M, Volkmer H
Frontiers in molecular neuroscience (2024) 17: 1480820. 135 511 ICC; tested species: rat

Regulation of synaptic connectivity in schizophrenia spectrum by mutual neuron-microglia interaction.
Breitmeyer R, Vogel S, Heider J, Hartmann SM, Wüst R, Keller AL, Binner A, Fitzgerald JC, Fallgatter AJ, Volkmer H
Communications biology (2023) 61: 472. 135 511 ICC; tested species: human

S-SCAM is essential for synapse formation.
Wittenmayer N, Petkova-Tuffy A, Borgmeyer M, Lee C, Becker J, Böning A, Kügler S, Rhee J, Viotti JS, Dresbach T
Frontiers in cellular neuroscience (2023) 17: 1182493. 135 511 ICC; tested species: rat

Vesicle cholesterol controls exocytotic fusion pore.
Rituper B, Guček A, Lisjak M, Gorska U, Šakanović A, Bobnar ST, Lasič E, Božić M, Abbineni PS, Jorgačevski J, Kreft M, et al.
Cell calcium (2022) 101: 102503. 135 511 ICC; tested species: rat

Autism Spectrum Disorder Risk Factor Met Regulates the Organization of Inhibitory Synapses.
Jeckel P, Kriebel M, Volkmer H
Frontiers in molecular neuroscience (2021) 14: 659856. 135 511 ICC; tested species: rat

Immunoprecipitation of Ribosome-Bound mRNAs from Astrocytic Perisynaptic Processes of the Mouse Hippocampus.
Mazaré N, Oudart M, Cheung G, Boulay AC, Cohen-Salmon M
STAR protocols (2020) 13: 100198. 135 511 ICC; tested species: mouse

Interference With Complex IV as a Model of Age-Related Decline in Synaptic Connectivity.
Kriebel M, Ebel J, Battke F, Griesbach S, Volkmer H
Frontiers in molecular neuroscience (2020) 13: 43. 135 511 ICC; tested species: rat

Presynaptic Endosomal Cathepsin D Regulates the Biogenesis of GABAergic Synaptic Vesicles.
Li X, Qin L, Li Y, Yu H, Zhang Z, Tao C, Liu Y, Xue Y, Zhang X, Xu Z, Wang Y, et al.
Cell reports (2019) 284: 1015-1028.e5. 135 511 ICC; tested species: rat

Presynaptic α2δ-2 Calcium Channel Subunits Regulate Postsynaptic GABAA Receptor Abundance and Axonal Wiring.
Geisler S, Schöpf CL, Stanika R, Kalb M, Campiglio M, Repetto D, Traxler L, Missler M, Obermair GJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) 3914: 2581-2605. 135 511 ICC; tested species: mouse

α-Neurexins Together with α2δ-1 Auxiliary Subunits Regulate Ca2+ Influx through Cav2.1 Channels.
Brockhaus J, Schreitmüller M, Repetto D, Klatt O, Reissner C, Elmslie K, Heine M, Missler M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3838: 8277-8294. 135 511 ICC; tested species: mouse

CDKL5 ensures excitatory synapse stability by reinforcing NGL-1-PSD95 interaction in the postsynaptic compartment and is impaired in patient iPSC-derived neurons.
Ricciardi S, Ungaro F, Hambrock M, Rademacher N, Stefanelli G, Brambilla D, Sessa A, Magagnotti C, Bachi A, Giarda E, Verpelli C, et al.
Nature cell biology (2012) 149: 911-23. 135 511 ICC; tested species: mouse

Postsynaptic TrkC and presynaptic PTPσ function as a bidirectional excitatory synaptic organizing complex.
Takahashi H, Arstikaitis P, Prasad T, Bartlett TE, Wang YT, Murphy TH, Craig AM
Neuron (2011) 692: 287-303. 135 511 ICC; tested species: rat

Telencephalic neurons monosynaptically link brainstem and forebrain premotor networks necessary for song.
Roberts TF, Klein ME, Kubke MF, Wild JM, Mooney R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2813: 3479-89. 135 511 ICC

Prefrontal parvalbumin interneurons mediate CRHR1-dependent early-life stress-induced cognitive deficits in adolescent male mice.
Ma YN, Yang CJ, Zhang CC, Sun YX, Yao XD, Liu X, Li XX, Wang HL, Wang H, Wang T, Wang XD, et al.
Molecular psychiatry (2024) : . 135 511 IHC; tested species: mouse

Deficits in neuronal architecture but not over-inhibition are main determinants of reduced neuronal network activity in a mouse model of overexpression of Dyrk1A.
Manubens-Gil L, Pons-Espinal M, Gener T, Ballesteros-Yañez I, de Lagrán MM, Dierssen M
Cerebral cortex (New York, N.Y. : 1991) (2024) 341: . 135 511 IHC; tested species: mouse

Deficits in neuronal architecture but not over-inhibition are main determinants of reduced neuronal network activity in a mouse model of overexpression of Dyrk1A.
Manubens-Gil L, Pons-Espinal M, Gener T, Ballesteros-Yañez I, Martínez de Lagrán M, Dierssen M
bioRxiv : the preprint server for biology (2023) : . 135 511 IHC; tested species: mouse

Ketogenic diet uncovers differential metabolic plasticity of brain cells.
Düking T, Spieth L, Berghoff SA, Piepkorn L, Schmidke AM, Mitkovski M, Kannaiyan N, Hosang L, Scholz P, Shaib AH, Schneider LV, et al.
Science advances (2022) 837: eabo7639. 135 511 IHC; tested species: mouse

Hippocampal Excitatory Synaptic Transmission and Plasticity Are Differentially Altered during Postnatal Development by Loss of the X-Linked Intellectual Disability Protein Oligophrenin-1.
Cresto N, Lebrun N, Dumont F, Letourneur F, Billuart P, Rouach N
Cells (2022) 119: . 135 511 IHC; tested species: mouse

Physiological synaptic activity and recognition memory require astroglial glutamine.
Cheung G, Bataveljic D, Visser J, Kumar N, Moulard J, Dallérac G, Mozheiko D, Rollenhagen A, Ezan P, Mongin C, Chever O, et al.
Nature communications (2022) 131: 753. 135 511 IHC; tested species: mouse

NaV1.1 haploinsufficiency impairs glutamatergic and GABAergic neuron function in the thalamus.
Studtmann C, Ladislav M, Topolski MA, Safari M, Swanger SA
Neurobiology of disease (2022) 167: 105672. 135 511 IHC; tested species: mouse

Increasing astrogenesis in the developing hippocampus induces autistic-like behavior in mice via enhancing inhibitory synaptic transmission.
Chen J, Ma XL, Zhao H, Wang XY, Xu MX, Wang H, Yang TQ, Peng C, Liu SS, Huang M, Zhou YD, et al.
Glia (2022) 701: 106-122. 135 511 IHC; tested species: mouse

Inhibition of Matrix Metalloproteinase 9 Activity Promotes Synaptogenesis in the Hippocampus.
Salamian A, Legutko D, Nowicka K, Badyra B, Kaźmierska-Grębowska P, Caban B, Kowalczyk T, Kaczmarek L, Beroun A
Cerebral cortex (New York, N.Y. : 1991) (2021) : . 135 511 IHC; tested species: rat

Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning.
Mazaré N, Oudart M, Moulard J, Cheung G, Tortuyaux R, Mailly P, Mazaud D, Bemelmans AP, Boulay AC, Blugeon C, Jourdren L, et al.
Cell reports (2020) 328: 108076. 135 511 IHC; tested species: mouse

Early pre- and postsynaptic decrease in glutamatergic and cholinergic signaling after spinalization is not modified when stimulating proprioceptive input to the ankle extensor α-motoneurons: Anatomical and neurochemical study.
Grycz K, Głowacka A, Ji B, Czarkowska-Bauch J, Gajewska-Woźniak O, Skup M
PloS one (2019) 149: e0222849. 135 511 IHC; tested species: rat

P/Q Type Calcium Channel Cav2.1 Defines a Unique Subset of Glomeruli in the Mouse Olfactory Bulb.
Pyrski M, Tusty M, Eckstein E, Oboti L, Rodriguez-Gil DJ, Greer CA, Zufall F
Frontiers in cellular neuroscience (2018) 12: 295. 135 511 IHC; tested species: mouse

Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 135 511 IHC; tested species: mouse

Distribution and development of molecularly distinct perineuronal nets in visual thalamus.
Sabbagh U, Monavarfeshani A, Su K, Zabet-Moghadam M, Cole J, Carnival E, Su J, Mirzaei M, Gupta V, Salekdeh GH, Fox MA, et al.
Journal of neurochemistry (2018) : . 135 511 IHC; tested species: mouse

LRRTM1 underlies synaptic convergence in visual thalamus.
Monavarfeshani A, Stanton G, Van Name J, Su K, Mills WA, Swilling K, Kerr A, Huebschman NA, Su J, Fox MA
eLife (2018) 7: . 135 511 IHC; tested species: mouse

Dorsal CA1 interneurons contribute to acute stress-induced spatial memory deficits.
Yu JY, Fang P, Wang C, Wang XX, Li K, Gong Q, Luo BY, Wang XD
Neuropharmacology (2018) : . 135 511 IHC; tested species: mouse

Expression pattern of synaptic vesicle protein 2 (SV2) isoforms in patients with temporal lobe epilepsy and hippocampal sclerosis.
Crèvecoeur J, Kaminski RM, Rogister B, Foerch P, Vandenplas C, Neveux M, Mazzuferi M, Kroonen J, Poulet C, Martin D, Sadzot B, et al.
Neuropathology and applied neurobiology (2014) 402: 191-204. 135 511 IHC; tested species: human

Hippocampal hyperexcitability underlies enhanced fear memories in TgNTRK3, a panic disorder mouse model.
Santos M, D'Amico D, Spadoni O, Amador-Arjona A, Stork O, Dierssen M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3338: 15259-71. 135 511 IHC; tested species: mouse

BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons.
Dieni S, Matsumoto T, Dekkers M, Rauskolb S, Ionescu MS, Deogracias R, Gundelfinger ED, Kojima M, Nestel S, Frotscher M, Barde YA, et al.
The Journal of cell biology (2012) 1966: 775-88. 135 511 IHC

Combined Treatment With Environmental Enrichment and (-)-Epigallocatechin-3-Gallate Ameliorates Learning Deficits and Hippocampal Alterations in a Mouse Model of Down Syndrome.
Catuara-Solarz S, Espinosa-Carrasco J, Erb I, Langohr K, Gonzalez JR, Notredame C, Dierssen M
eNeuro () 35: . 135 511 IHC; tested species: mouse

Functional Neuroligin-2-MDGA1 interactions differentially regulate synaptic GABAARs and cytosolic gephyrin aggregation.
Zeppillo T, Ali H, Ravichandran S, Ritter TC, Wenger S, López-Murcia FJ, Gideons E, Signorelli J, Schmeisser MJ, Wiltfang J, Rhee J, et al.
Communications biology (2024) 71: 1157. 135 511 IHC-Fr; tested species: mouse

Brain Iron Deficiency Changes the Stoichiometry of Adenosine Receptor Subtypes in Cortico-Striatal Terminals: Implications for Restless Legs Syndrome.
Rodrigues MS, Ferreira SG, Quiroz C, Earley CJ, García-Borreguero D, Cunha RA, Ciruela F, Köfalvi A, Ferré S
Molecules (Basel, Switzerland) (2022) 275: . 135 511 FACS; tested species: rat

Cat. No.: 135 511

Amount: 100 µg

Price: $420.00

Deficits in neuronal architecture but not over-inhibition are main determinants of reduced neuronal network activity in a mouse model of overexpression of Dyrk1A.
Manubens-Gil L, Pons-Espinal M, Gener T, Ballesteros-Yañez I, Martínez de Lagrán M, Dierssen M
bioRxiv : the preprint server for biology (2023) : . 135 511 IHC; tested species: mouse

ICC

IHC

IHC-P

Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.

1 ) Takamori S et al., Nature (2000)
2 ) Bellocchio EE et al., Science (2000)
3 ) Bellocchio EE et al., J. Neurosci. (1998)
4 ) Ni B et al., Proc. Natl. Acad. Sci. U.S.A. (1994)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

VGLUT1 antibody - 135 511 K.O.

References for VGLUT1 - 135 511