VPS18 antibody - 321 003

VPS18 is a peripheral membrane protein associated with late endosomes/lysosomes

Rabbit polyclonal purified antibody

Cat. No.: 321 003

Amount: 50 µg

Price: $375.00

Cat. No. 321 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 956 to 973 from mouse VPS18 (UniProt Id: Q8R307)
Reactivity	Reacts with: rat (B5DFJ4), mouse (Q8R307). Other species not tested yet.
Matching control protein/peptide	321-0P
Data sheet	321_003.pdf

Cat. No.: 321 003

Amount: 50 µg

Price: $375.00

Background

In yeast the class C vacuole protein sorting (VPS) proteins VPS 11, 16, 18 and 33 interact with SNARE and rab proteins to "tether" vacuolar membranes before fusion.

1 ) Richardson SC et al., Mol. Biol. Cell (2004)
2 ) Kim BY et al., J. Biol. Chem. (2001)

Protocols

Standard protocol

OTHER

Preabsorbtion

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