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VAMP7 antibody - 232 011 K.O.

Vamp7 also known as Ti-Vamp is a tetanus neurotoxin-insensitive vesicle-associated membrane protein
Mouse monoclonal purified IgG
Cat. No.: 232 011
Amount: 100 µg
Price: $415.00
Cat. No. 232 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: not tested yet gallery  
IHC: not tested yet gallery  
IHC-P: not tested yet
Clone 158.2
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 119 to 188 from mouse VAMP7 (UniProt Id: P70280)
Reactivity Reacts with: rat (Q9JHW5), mouse (P70280), human (P51809), chicken.
No signal: zebrafish.
Other species not tested yet.
Specificity K.O. validated PubMed: 30271964
Data sheet 232_011.pdf

References for VAMP7 - 232 011

Comparative study of commercially available and homemade anti-VAMP7 antibodies using CRISPR/Cas9-depleted HeLa cells and VAMP7 knockout mice.
Verraes A, Cholley B, Galli T, Nola S
F1000Research (2018) 7: 1649. 232 011 WB; KO verified; tested species: mouse
Vesicle-associated membrane protein 7-mediated eosinophil degranulation promotes allergic airway inflammation in mice.
Willetts L, Felix LC, Jacobsen EA, Puttagunta L, Condjella RM, Zellner KR, Ochkur SI, Kim JD, Luo H, Lee NA, Lee JJ, et al.
Communications biology (2018) 1: 83. 232 011 WB, ICC; KO verified; tested species: human
Cyclic Nucleotide Control of Microtubule Dynamics for Axon Guidance.
Akiyama H, Fukuda T, Tojima T, Nikolaev VO, Kamiguchi H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3620: 5636-49. 232 011 WB; KD verified; tested species: chicken
VAMP-7 links granule exocytosis to actin reorganization during platelet activation.
Koseoglu S, Peters CG, Fitch-Tewfik JL, Aisiku O, Danglot L, Galli T, Flaumenhaft R
Blood (2015) 1265: 651-60. 232 011 WB
Tetanus neurotoxin-insensitive vesicle-associated membrane protein localizes to a presynaptic membrane compartment in selected terminal subsets of the rat brain.
Muzerelle A, Alberts P, Martinez-Arca S, Jeannequin O, Lafaye P, Mazié JC, Galli T, Gaspar P
Neuroscience (2003) 1221: 59-75. 232 011 WB, IHC
Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 232 011 IP; tested species: mouse
Vesicle-associated membrane protein 7-mediated eosinophil degranulation promotes allergic airway inflammation in mice.
Willetts L, Felix LC, Jacobsen EA, Puttagunta L, Condjella RM, Zellner KR, Ochkur SI, Kim JD, Luo H, Lee NA, Lee JJ, et al.
Communications biology (2018) 1: 83. 232 011 WB, ICC; KO verified; tested species: human
An actin cytoskeletal barrier inhibits lytic granule release from natural killer cells in patients with Chediak-Higashi syndrome.
Gil-Krzewska A, Saeed MB, Oszmiana A, Fischer ER, Lagrue K, Gahl WA, Introne WJ, Coligan JE, Davis DM, Krzewski K
The Journal of allergy and clinical immunology (2017) : . 232 011 ICC; tested species: human
Human autoantibodies to amphiphysin induce defective presynaptic vesicle dynamics and composition.
Werner C, Pauli M, Doose S, Weishaupt A, Haselmann H, Grünewald B, Sauer M, Heckmann M, Toyka KV, Asan E, Sommer C, et al.
Brain : a journal of neurology (2016) 139Pt 2: 365-79. 232 011 ICC; tested species: rat
Tetanus neurotoxin-insensitive vesicle-associated membrane protein localizes to a presynaptic membrane compartment in selected terminal subsets of the rat brain.
Muzerelle A, Alberts P, Martinez-Arca S, Jeannequin O, Lafaye P, Mazié JC, Galli T, Gaspar P
Neuroscience (2003) 1221: 59-75. 232 011 WB, IHC
Cat. No.: 232 011
Amount: 100 µg
Price: $415.00
Comparative study of commercially available and homemade anti-VAMP7 antibodies using CRISPR/Cas9-depleted HeLa cells and VAMP7 knockout mice.
Verraes A, Cholley B, Galli T, Nola S
F1000Research (2018) 7: 1649. 232 011 WB; KO verified; tested species: mouse
Vesicle-associated membrane protein 7-mediated eosinophil degranulation promotes allergic airway inflammation in mice.
Willetts L, Felix LC, Jacobsen EA, Puttagunta L, Condjella RM, Zellner KR, Ochkur SI, Kim JD, Luo H, Lee NA, Lee JJ, et al.
Communications biology (2018) 1: 83. 232 011 WB, ICC; KO verified; tested species: human
Cyclic Nucleotide Control of Microtubule Dynamics for Axon Guidance.
Akiyama H, Fukuda T, Tojima T, Nikolaev VO, Kamiguchi H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3620: 5636-49. 232 011 WB; KD verified; tested species: chicken
VAMP-7 links granule exocytosis to actin reorganization during platelet activation.
Koseoglu S, Peters CG, Fitch-Tewfik JL, Aisiku O, Danglot L, Galli T, Flaumenhaft R
Blood (2015) 1265: 651-60. 232 011 WB
Tetanus neurotoxin-insensitive vesicle-associated membrane protein localizes to a presynaptic membrane compartment in selected terminal subsets of the rat brain.
Muzerelle A, Alberts P, Martinez-Arca S, Jeannequin O, Lafaye P, Mazié JC, Galli T, Gaspar P
Neuroscience (2003) 1221: 59-75. 232 011 WB, IHC
Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 232 011 IP; tested species: mouse
Vesicle-associated membrane protein 7-mediated eosinophil degranulation promotes allergic airway inflammation in mice.
Willetts L, Felix LC, Jacobsen EA, Puttagunta L, Condjella RM, Zellner KR, Ochkur SI, Kim JD, Luo H, Lee NA, Lee JJ, et al.
Communications biology (2018) 1: 83. 232 011 WB, ICC; KO verified; tested species: human
An actin cytoskeletal barrier inhibits lytic granule release from natural killer cells in patients with Chediak-Higashi syndrome.
Gil-Krzewska A, Saeed MB, Oszmiana A, Fischer ER, Lagrue K, Gahl WA, Introne WJ, Coligan JE, Davis DM, Krzewski K
The Journal of allergy and clinical immunology (2017) : . 232 011 ICC; tested species: human
Human autoantibodies to amphiphysin induce defective presynaptic vesicle dynamics and composition.
Werner C, Pauli M, Doose S, Weishaupt A, Haselmann H, Grünewald B, Sauer M, Heckmann M, Toyka KV, Asan E, Sommer C, et al.
Brain : a journal of neurology (2016) 139Pt 2: 365-79. 232 011 ICC; tested species: rat
Tetanus neurotoxin-insensitive vesicle-associated membrane protein localizes to a presynaptic membrane compartment in selected terminal subsets of the rat brain.
Muzerelle A, Alberts P, Martinez-Arca S, Jeannequin O, Lafaye P, Mazié JC, Galli T, Gaspar P
Neuroscience (2003) 1221: 59-75. 232 011 WB, IHC
Background

VAMP 7, also referred to as Ti-VAMP and SybL 1, is a member of the SNARE family of proteins and a relative of synaptobrevin. It is involved in membrane fusion events that mediate neurite outgrowth in developing neurons, in endosome to lysosome transport and in other cellular trafficking mechanisms. VAMP 7 is ubiquitously expressed in different tissues.
It is a member of the syntaxin 4-SNAP 23-VAMP 7- and the syntaxin 7-syntaxin 8-Vti1b-VAMP 7-SNARE complex.