Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

USP9x antibody - 285 002

USP9x is a deubiquitinase belonging to the UBP family
Rabbit polyclonal antiserum
Cat. No.: 285 002
Amount: 200 µl
Price: $355.00
Cat. No. 285 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to AA 1 to 91 from mouse USP9x (UniProt Id: P70398)
Reactivity Reacts with: rat, mouse (P70398).
Other species not tested yet.
Data sheet 285_002.pdf
Cat. No.: 285 002
Amount: 200 µl
Price: $355.00
Background

Ubiquitinylation of proteins regulates their stability and turnover and is involved in many cellular processes like endocytosis, cell-cycle regulation, transcriptional activation, etc.
Ubiquitin can be removed by deubiquitylating enzymes (DUBs) comprising ubiquitin specific proteases (USPs), or ubiquitin C-terminal hydrolases (UCHs).
Fat facets in mouse (FAM, also known as Usp9X) is a developmentally regulated UBP modulating ubiquitinylation of proteins like β-catenin, α-synuclein and others.