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Home > Products > 149 011

Uncoating ATPase antibody - 149 011

Uncoating ATPase is involved in the removal of the clathrin coat of vesicles
Mouse monoclonal purified IgG
Cat. No.: 149 011
Amount: 100 µg
Price: $415.00
Cat. No. 149 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: yes
IHC-P: 1 : 200 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 3C5
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 650 from bovine Uncoating ATPase (UniProt Id: P19120)
Epitop AA 391 to 546 from bovine Uncoating ATPase (UniProt Id: P19120)
Reactivity Reacts with: human (P11142), rat (P63018), mouse (P63017), hamster, cow.
Other species not tested yet.
Remarks

When microinjected into cells, the antibody inhibits clathrin mediated endocytosis.
Data sheet 149_011.pdf

References for Uncoating ATPase - 149 011

Hsc70 phosphorylation patterns and calmodulin regulate AP2 CCV life span for cell adhesion protein transport.
Sengül GF, Mishra R, Candiello E, Schu P
Biochimica et biophysica acta. Molecular cell research (2023) : 119611. 149 011 WB; tested species: mouse
CSPα reduces aggregates and rescues striatal dopamine release in α-synuclein transgenic mice.
Caló L, Hidari E, Wegrzynowicz M, Dalley JW, Schneider BL, Podgajna M, Anichtchik O, Carlson E, Klenerman D, Spillantini MG
Brain : a journal of neurology (2021) 1446: 1661-1669. 149 011 WB; tested species: mouse
Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.
Naseri NN, Ergel B, Kharel P, Na Y, Huang Q, Huang R, Dolzhanskaya N, Burré J, Velinov MT, Sharma M
Nature structural & molecular biology (2020) 272: 192-201. 149 011 WB; tested species: mouse
Lysosomal dysfunction disrupts presynaptic maintenance and restoration of presynaptic function prevents neurodegeneration in lysosomal storage diseases.
Sambri I, D'Alessio R, Ezhova Y, Giuliano T, Sorrentino NC, Cacace V, De Risi M, Cataldi M, Annunziato L, De Leonibus E, Fraldi A, et al.
EMBO molecular medicine (2017) 91: 112-132. 149 011 WB; tested species: mouse
A mouse model for fucosidosis recapitulates storage pathology and neurological features of the milder form of the human disease.
Wolf H, Damme M, Stroobants S, D'Hooge R, Beck HC, Hermans-Borgmeyer I, Lüllmann-Rauch R, Dierks T, Lübke T
Disease models & mechanisms (2016) 99: 1015-28. 149 011 WB; tested species: mouse
The SNARE protein vti1a functions in dense-core vesicle biogenesis.
Walter AM, Kurps J, de Wit H, Schöning S, Toft-Bertelsen TL, Lauks J, Ziomkiewicz I, Weiss AN, Schulz A, Fischer von Mollard G, Verhage M, et al.
The EMBO journal (2014) 3315: 1681-97. 149 011 WB; tested species: mouse
Synaptic function of nicastrin in hippocampal neurons.
Lee SH, Sharma M, Südhof TC, Shen J
Proceedings of the National Academy of Sciences of the United States of America (2014) 11124: 8973-8. 149 011 WB; tested species: mouse
CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 149 011 WB; tested species: mouse
CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity.
Sharma M, Burré J, Südhof TC
Nature cell biology (2011) 131: 30-9. 149 011 WB; tested species: mouse
Cysteine string protein-alpha prevents activity-dependent degeneration in GABAergic synapses.
García-Junco-Clemente P, Cantero G, Gómez-Sánchez L, Linares-Clemente P, Martínez-López JA, Luján R, Fernández-Chacón R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3021: 7377-91. 149 011 WB
Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro.
Burré J, Sharma M, Tsetsenis T, Buchman V, Etherton MR, Südhof TC
Science (New York, N.Y.) (2010) 3295999: 1663-7. 149 011 WB
Quantitative proteomics analysis of cell cycle-regulated Golgi disassembly and reassembly.
Chen X, Simon ES, Xiang Y, Kachman M, Andrews PC, Wang Y
The Journal of biological chemistry (2010) 28510: 7197-207. 149 011 WB; tested species: rat
Dominant-interfering Hsc70 mutants disrupt multiple stages of the clathrin-coated vesicle cycle in vivo.
Newmyer SL, Schmid SL
The Journal of cell biology (2001) 1523: 607-20. 149 011 WB
Synaptic AP2 CCV life cycle regulation by the Eps15, ITSN1, Sgip1/AP2, synaptojanin1 interactome.
Mishra R, Sengül GF, Candiello E, Schu P
Scientific reports (2021) 111: 8007. 149 011 IP; tested species: mouse
Chaperone-mediated autophagy in neuronal dendrites utilizes activity-dependent lysosomal exocytosis for protein disposal.
Grochowska KM, Sperveslage M, Raman R, Failla AV, Głów D, Schulze C, Laprell L, Fehse B, Kreutz MR
Cell reports (2023) 428: 112998. 149 011 ICC; tested species: rat
Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 149 011 IHC; tested species: mouse
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 149 011 IHC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 149 011 IHC; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 149 011
ATP- and cytosol-dependent release of adaptor proteins from clathrin-coated vesicles: A dual role for Hsc70.
Hannan LA, Newmyer SL, Schmid SL
Molecular biology of the cell (1998) 98: 2217-29. 149 011
Receptor-mediated endocytosis is sensitive to antibodies against the uncoating ATPase (hsc70).
Höning S, Kreimer G, Robenek H, Jockusch BM
Journal of cell science (1994) 107 ( Pt 5): 1185-96. 149 011
Cat. No.: 149 011
Amount: 100 µg
Price: $415.00
Hsc70 phosphorylation patterns and calmodulin regulate AP2 CCV life span for cell adhesion protein transport.
Sengül GF, Mishra R, Candiello E, Schu P
Biochimica et biophysica acta. Molecular cell research (2023) : 119611. 149 011 WB; tested species: mouse
CSPα reduces aggregates and rescues striatal dopamine release in α-synuclein transgenic mice.
Caló L, Hidari E, Wegrzynowicz M, Dalley JW, Schneider BL, Podgajna M, Anichtchik O, Carlson E, Klenerman D, Spillantini MG
Brain : a journal of neurology (2021) 1446: 1661-1669. 149 011 WB; tested species: mouse
Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.
Naseri NN, Ergel B, Kharel P, Na Y, Huang Q, Huang R, Dolzhanskaya N, Burré J, Velinov MT, Sharma M
Nature structural & molecular biology (2020) 272: 192-201. 149 011 WB; tested species: mouse
Lysosomal dysfunction disrupts presynaptic maintenance and restoration of presynaptic function prevents neurodegeneration in lysosomal storage diseases.
Sambri I, D'Alessio R, Ezhova Y, Giuliano T, Sorrentino NC, Cacace V, De Risi M, Cataldi M, Annunziato L, De Leonibus E, Fraldi A, et al.
EMBO molecular medicine (2017) 91: 112-132. 149 011 WB; tested species: mouse
A mouse model for fucosidosis recapitulates storage pathology and neurological features of the milder form of the human disease.
Wolf H, Damme M, Stroobants S, D'Hooge R, Beck HC, Hermans-Borgmeyer I, Lüllmann-Rauch R, Dierks T, Lübke T
Disease models & mechanisms (2016) 99: 1015-28. 149 011 WB; tested species: mouse
The SNARE protein vti1a functions in dense-core vesicle biogenesis.
Walter AM, Kurps J, de Wit H, Schöning S, Toft-Bertelsen TL, Lauks J, Ziomkiewicz I, Weiss AN, Schulz A, Fischer von Mollard G, Verhage M, et al.
The EMBO journal (2014) 3315: 1681-97. 149 011 WB; tested species: mouse
Synaptic function of nicastrin in hippocampal neurons.
Lee SH, Sharma M, Südhof TC, Shen J
Proceedings of the National Academy of Sciences of the United States of America (2014) 11124: 8973-8. 149 011 WB; tested species: mouse
CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 149 011 WB; tested species: mouse
CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity.
Sharma M, Burré J, Südhof TC
Nature cell biology (2011) 131: 30-9. 149 011 WB; tested species: mouse
Cysteine string protein-alpha prevents activity-dependent degeneration in GABAergic synapses.
García-Junco-Clemente P, Cantero G, Gómez-Sánchez L, Linares-Clemente P, Martínez-López JA, Luján R, Fernández-Chacón R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3021: 7377-91. 149 011 WB
Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro.
Burré J, Sharma M, Tsetsenis T, Buchman V, Etherton MR, Südhof TC
Science (New York, N.Y.) (2010) 3295999: 1663-7. 149 011 WB
Quantitative proteomics analysis of cell cycle-regulated Golgi disassembly and reassembly.
Chen X, Simon ES, Xiang Y, Kachman M, Andrews PC, Wang Y
The Journal of biological chemistry (2010) 28510: 7197-207. 149 011 WB; tested species: rat
Dominant-interfering Hsc70 mutants disrupt multiple stages of the clathrin-coated vesicle cycle in vivo.
Newmyer SL, Schmid SL
The Journal of cell biology (2001) 1523: 607-20. 149 011 WB
Synaptic AP2 CCV life cycle regulation by the Eps15, ITSN1, Sgip1/AP2, synaptojanin1 interactome.
Mishra R, Sengül GF, Candiello E, Schu P
Scientific reports (2021) 111: 8007. 149 011 IP; tested species: mouse
Chaperone-mediated autophagy in neuronal dendrites utilizes activity-dependent lysosomal exocytosis for protein disposal.
Grochowska KM, Sperveslage M, Raman R, Failla AV, Głów D, Schulze C, Laprell L, Fehse B, Kreutz MR
Cell reports (2023) 428: 112998. 149 011 ICC; tested species: rat
Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 149 011 IHC; tested species: mouse
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 149 011 IHC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 149 011 IHC; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 149 011
ATP- and cytosol-dependent release of adaptor proteins from clathrin-coated vesicles: A dual role for Hsc70.
Hannan LA, Newmyer SL, Schmid SL
Molecular biology of the cell (1998) 98: 2217-29. 149 011
Receptor-mediated endocytosis is sensitive to antibodies against the uncoating ATPase (hsc70).
Höning S, Kreimer G, Robenek H, Jockusch BM
Journal of cell science (1994) 107 ( Pt 5): 1185-96. 149 011
WB
ICC
IHC-P
Background

Clathrin coated vesicles are involved in a wide variety of transport events of the eucaryotic cell. This includes receptor mediated endocytosis or the recycling of synaptic vesicles in nerve terminals.
Before the vesicles can fuse with the endosomal compartment the clathrin coat has to be removed in an ATP dependent process. Uncoating ATPase (UA), also referred to as hsc70, has been shown to be functionally involved in this process.
Uncoating ATPase belongs to the heatshock protein (hsp70) family. Most proteins of this family are molecular chaperones and the uncoating ATPase shares the ability to refold proteins in several cellular processes.