UCHL1 antibody - 233 003

UCHL1 or PGP9.5 is a neuron specific member of the ubiquitin proteasome system

Rabbit polyclonal purified antibody

Cat. No.: 233 003

Amount: 50 µg

Price: $455.00

Cat. No. 233 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: yes gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 2 to 223 from mouse UCHL1 (UniProt Id: Q9R0P9)
Reactivity	Reacts with: rat (Q00981), mouse (Q9R0P9). No signal: zebrafish. Other species not tested yet.
Data sheet	233_003.pdf

Cat. No.: 233 003

Amount: 50 µg

Price: $455.00

ICC

IHC

Background

The Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL 1) enzyme is a member of a protein family involved in the hydrolyzation of C-terminal adducts of polymeric ubiquitin to generate monomeric ubiquitin. It is abundantly expressed in neurons throughout the brain and belongs to the ubiquitin-proteasome system. This pathway is responsible for the degradation of misfolded and damaged proteins and has been postulated to play an important role in Parkinson's disease.

1 ) Ragland M et al., Am. J. Epidemiol. (2009)
2 ) Xu EH et al., J Clin Neurosci (2009)
3 ) Zhang ZJ et al., Neurosci. Lett. (2008)
4 ) Hutter CM et al., Eur. J. Neurol. (2008)
5 ) Lombardino AJ et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
6 ) Otsuki T et al., Br. J. Haematol. (2004)
7 ) Satoh JI et al., Neuropathol. Appl. Neurobiol. (2001)
8 ) Trowern AR et al., Neurosci. Lett. (1996)

Protocols

ICC

IHC

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