UBE3B antibody - 339 003

UBE 3B is an ubiquitin ligase

Rabbit polyclonal purified antibody

Cat. No.: 339 003

Amount: 50 µg

Price: $375.00

Cat. No. 339 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 707 to 1072 from mouse UBE3B (UniProt Id: Q9ES34)
Reactivity	Reacts with: rat, mouse (Q9ES34). Other species not tested yet.
Specificity	Detects a protein of the expected size in hippocampus homogenate.
Data sheet	339_003.pdf

Cat. No.: 339 003

Amount: 50 µg

Price: $375.00

Background

UBE 3B is an E3 ubiquitin ligase and accepts ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then transfers it to targeted substrates.
Loss of function mutations of this protein are associated with Kaufman oculocerebrofacial syndrome.

1 ) Flex E et al., J. Med. Genet. (2013)
2 ) Basel-Vanagaite L et al., Am. J. Hum. Genet. (2012)
3 ) Gong TW et al., Genomics (2003)

Protocols

Standard protocol