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Home > Products > 203 011

U1-70k antibody - 203 011

U1-70k is a member of the U1 snRNP
Mouse monoclonal purified IgG
Cat. No.: 203 011
Amount: 100 µg
Price: $415.00
Cat. No. 203 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: yes
IHC-P: 1 : 500 gallery  
FACS: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.
Clone H111
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 437 from human U1-70k (UniProt Id: P08621)
Epitop AA 1 to 275 from human U1-70k (UniProt Id: P08621)
Reactivity Reacts with: human (P08621), rat, mouse (Q62376), mammals.
Other species not tested yet.
Data sheet 203_011.pdf

References for U1-70k - 203 011

Autonomous transposons tune their sequences to ensure somatic suppression.
Ilık İA, Glažar P, Tse K, Brändl B, Meierhofer D, Müller FJ, Smith ZD, Aktaş T
Nature (2024) : . 203 011 WB; tested species: human
Splicing regulation of GFPT1 muscle-specific isoform and its roles in glucose metabolisms and neuromuscular junction.
Farshadyeganeh P, Nazim M, Zhang R, Ohkawara B, Nakajima K, Rahman MA, Nasrin F, Ito M, Takeda JI, Ohe K, Miyasaka Y, et al.
iScience (2023) 2610: 107746. 203 011 WB; tested species: mouse
Stimulus-specific remodeling of the neuronal transcriptome through nuclear intron-retaining transcripts.
Mazille M, Buczak K, Scheiffele P, Mauger O
The EMBO journal (2022) : e110192. 203 011 WB; tested species: mouse
tRIP-seq reveals repression of premature polyadenylation by co-transcriptional FUS-U1 snRNP assembly.
Masuda A, Kawachi T, Takeda JI, Ohkawara B, Ito M, Ohno K
EMBO reports (2020) 215: e49890. 203 011 WB, IP; tested species: mouse
Synergistic assembly of human pre-spliceosomes across introns and exons.
Braun JE, Friedman LJ, Gelles J, Moore MJ
eLife (2018) 7: . 203 011 WB; tested species: human
Post-transcriptional Regulation of De Novo Lipogenesis by mTORC1-S6K1-SRPK2 Signaling.
Lee G, Zheng Y, Cho S, Jang C, England C, Dempsey JM, Yu Y, Liu X, He L, Cavaliere PM, Chavez A, et al.
Cell (2017) 1717: 1545-1558.e18. 203 011 WB; tested species: human
Accumulation of nuclear ADAR2 regulates adenosine-to-inosine RNA editing during neuronal development.
Behm M, Wahlstedt H, Widmark A, Eriksson M, Öhman M
Journal of cell science (2017) 1304: 745-753. 203 011 WB; tested species: mouse
The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators.
Llorian M, Gooding C, Bellora N, Hallegger M, Buckroyd A, Wang X, Rajgor D, Kayikci M, Feltham J, Ule J, Eyras E, et al.
Nucleic acids research (2016) 4418: 8933-8950. 203 011 WB
A 10S galectin-3-U1 snRNP complex assembles into active spliceosomes.
Haudek KC, Voss PG, Wang JL, Patterson RJ
Nucleic acids research (2016) 4413: 6391-7. 203 011 WB
SRSF1 and hnRNP H antagonistically regulate splicing of COLQ exon 16 in a congenital myasthenic syndrome.
Rahman MA, Azuma Y, Nasrin F, Takeda J, Nazim M, Bin Ahsan K, Masuda A, Engel AG, Ohno K
Scientific reports (2015) 5: 13208. 203 011 WB
Detained introns are a novel, widespread class of post-transcriptionally spliced introns.
Boutz PL, Bhutkar A, Sharp PA
Genes & development (2015) 291: 63-80. 203 011 WB; tested species: mouse
A novel intra-U1 snRNP cross-regulation mechanism: alternative splicing switch links U1C and U1-70K expression.
Rösel-Hillgärtner TD, Hung LH, Khrameeva E, Le Querrec P, Gelfand MS, Bindereif A
PLoS genetics (2013) 910: e1003856. 203 011 WB; KD verified; tested species: human
Heterogeneous nuclear ribonucleoprotein A1 interferes with the binding of the human T cell leukemia virus type 1 rex regulatory protein to its response element.
Dodon MD, Hamaia S, Martin J, Gazzolo L
The Journal of biological chemistry (2002) 27721: 18744-52. 203 011 WB
Structure of the small nuclear RNP particle U1: identification of the two structural protuberances with RNP-antigens A and 70K.
Kastner B, Kornstädt U, Bach M, Lührmann R
The Journal of cell biology (1992) 1164: 839-49. 203 011 WB
tRIP-seq reveals repression of premature polyadenylation by co-transcriptional FUS-U1 snRNP assembly.
Masuda A, Kawachi T, Takeda JI, Ohkawara B, Ito M, Ohno K
EMBO reports (2020) 215: e49890. 203 011 WB, IP; tested species: mouse
DNMT1-associated long non-coding RNAs regulate global gene expression and DNA methylation in colon cancer.
Merry CR, Forrest ME, Sabers JN, Beard L, Gao XH, Hatzoglou M, Jackson MW, Wang Z, Markowitz SD, Khalil AM
Human molecular genetics (2015) 2421: 6240-53. 203 011 IP
Spatial mapping of splicing factor complexes involved in exon and intron definition.
Ellis JD, Llères D, Denegri M, Lamond AI, Cáceres JF
The Journal of cell biology (2008) 1816: 921-34. 203 011 IP
p54(nrb) associates with the 5' splice site within large transcription/splicing complexes.
Kameoka S, Duque P, Konarska MM
The EMBO journal (2004) 238: 1782-91. 203 011 IP
A general approach for identification of RNA-protein cross-linking sites within native human spliceosomal small nuclear ribonucleoproteins (snRNPs). Analysis of RNA-protein contacts in native U1 and U4/U6.U5 snRNPs.
Urlaub H, Hartmuth K, Kostka S, Grelle G, Lührmann R
The Journal of biological chemistry (2000) 27552: 41458-68. 203 011 IP
AU-rich elements target small nuclear RNAs as well as mRNAs for rapid degradation.
Fan XC, Myer VE, Steitz JA
Genes & development (1997) 1119: 2557-68. 203 011 IP
Evidence for a direct role of the disease modifier SCNM1 in splicing.
Howell VM, Jones JM, Bergren SK, Li L, Billi AC, Avenarius MR, Meisler MH
Human molecular genetics (2007) 1620: 2506-16. 203 011 ICC
Synergistic enhancement of production of proinflammatory cytokines of human peripheral blood monocytes by anti-Sm and anti-RNP antibodies.
Matsueda Y, Arinuma Y, Nagai T, Hirohata S
PloS one (2018) 1312: e0209282. 203 011 FACS; tested species: human
Three-dimensional structure of a pre-catalytic human spliceosomal complex B.
Boehringer D, Makarov EM, Sander B, Makarova OV, Kastner B, Lührmann R, Stark H
Nature structural & molecular biology (2004) 115: 463-8. 203 011
High intranuclear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle tracking.
Kues T, Dickmanns A, Lührmann R, Peters R, Kubitscheck U
Proceedings of the National Academy of Sciences of the United States of America (2001) 9821: 12021-6. 203 011
Detection of a novel ATP-dependent cross-linked protein at the 5' splice site-U1 small nuclear RNA duplex by methylene blue-mediated photo-cross-linking.
Liu ZR, Sargueil B, Smith CW
Molecular and cellular biology (1998) 1812: 6910-20. 203 011
Identification of a human protein that recognizes the 3' splice site during the second step of pre-mRNA splicing.
Wu S, Green MR
The EMBO journal (1997) 1614: 4421-32. 203 011
The association of the U1-specific 70K and C proteins with U1 snRNPs is mediated in part by common U snRNP proteins.
Nelissen RL, Will CL, van Venrooij WJ, Lührmann R
The EMBO journal (1994) 1317: 4113-25. 203 011
Cat. No.: 203 011
Amount: 100 µg
Price: $415.00
Autonomous transposons tune their sequences to ensure somatic suppression.
Ilık İA, Glažar P, Tse K, Brändl B, Meierhofer D, Müller FJ, Smith ZD, Aktaş T
Nature (2024) : . 203 011 WB; tested species: human
Splicing regulation of GFPT1 muscle-specific isoform and its roles in glucose metabolisms and neuromuscular junction.
Farshadyeganeh P, Nazim M, Zhang R, Ohkawara B, Nakajima K, Rahman MA, Nasrin F, Ito M, Takeda JI, Ohe K, Miyasaka Y, et al.
iScience (2023) 2610: 107746. 203 011 WB; tested species: mouse
Stimulus-specific remodeling of the neuronal transcriptome through nuclear intron-retaining transcripts.
Mazille M, Buczak K, Scheiffele P, Mauger O
The EMBO journal (2022) : e110192. 203 011 WB; tested species: mouse
tRIP-seq reveals repression of premature polyadenylation by co-transcriptional FUS-U1 snRNP assembly.
Masuda A, Kawachi T, Takeda JI, Ohkawara B, Ito M, Ohno K
EMBO reports (2020) 215: e49890. 203 011 WB, IP; tested species: mouse
Synergistic assembly of human pre-spliceosomes across introns and exons.
Braun JE, Friedman LJ, Gelles J, Moore MJ
eLife (2018) 7: . 203 011 WB; tested species: human
Post-transcriptional Regulation of De Novo Lipogenesis by mTORC1-S6K1-SRPK2 Signaling.
Lee G, Zheng Y, Cho S, Jang C, England C, Dempsey JM, Yu Y, Liu X, He L, Cavaliere PM, Chavez A, et al.
Cell (2017) 1717: 1545-1558.e18. 203 011 WB; tested species: human
Accumulation of nuclear ADAR2 regulates adenosine-to-inosine RNA editing during neuronal development.
Behm M, Wahlstedt H, Widmark A, Eriksson M, Öhman M
Journal of cell science (2017) 1304: 745-753. 203 011 WB; tested species: mouse
The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators.
Llorian M, Gooding C, Bellora N, Hallegger M, Buckroyd A, Wang X, Rajgor D, Kayikci M, Feltham J, Ule J, Eyras E, et al.
Nucleic acids research (2016) 4418: 8933-8950. 203 011 WB
A 10S galectin-3-U1 snRNP complex assembles into active spliceosomes.
Haudek KC, Voss PG, Wang JL, Patterson RJ
Nucleic acids research (2016) 4413: 6391-7. 203 011 WB
SRSF1 and hnRNP H antagonistically regulate splicing of COLQ exon 16 in a congenital myasthenic syndrome.
Rahman MA, Azuma Y, Nasrin F, Takeda J, Nazim M, Bin Ahsan K, Masuda A, Engel AG, Ohno K
Scientific reports (2015) 5: 13208. 203 011 WB
Detained introns are a novel, widespread class of post-transcriptionally spliced introns.
Boutz PL, Bhutkar A, Sharp PA
Genes & development (2015) 291: 63-80. 203 011 WB; tested species: mouse
A novel intra-U1 snRNP cross-regulation mechanism: alternative splicing switch links U1C and U1-70K expression.
Rösel-Hillgärtner TD, Hung LH, Khrameeva E, Le Querrec P, Gelfand MS, Bindereif A
PLoS genetics (2013) 910: e1003856. 203 011 WB; KD verified; tested species: human
Heterogeneous nuclear ribonucleoprotein A1 interferes with the binding of the human T cell leukemia virus type 1 rex regulatory protein to its response element.
Dodon MD, Hamaia S, Martin J, Gazzolo L
The Journal of biological chemistry (2002) 27721: 18744-52. 203 011 WB
Structure of the small nuclear RNP particle U1: identification of the two structural protuberances with RNP-antigens A and 70K.
Kastner B, Kornstädt U, Bach M, Lührmann R
The Journal of cell biology (1992) 1164: 839-49. 203 011 WB
tRIP-seq reveals repression of premature polyadenylation by co-transcriptional FUS-U1 snRNP assembly.
Masuda A, Kawachi T, Takeda JI, Ohkawara B, Ito M, Ohno K
EMBO reports (2020) 215: e49890. 203 011 WB, IP; tested species: mouse
DNMT1-associated long non-coding RNAs regulate global gene expression and DNA methylation in colon cancer.
Merry CR, Forrest ME, Sabers JN, Beard L, Gao XH, Hatzoglou M, Jackson MW, Wang Z, Markowitz SD, Khalil AM
Human molecular genetics (2015) 2421: 6240-53. 203 011 IP
Spatial mapping of splicing factor complexes involved in exon and intron definition.
Ellis JD, Llères D, Denegri M, Lamond AI, Cáceres JF
The Journal of cell biology (2008) 1816: 921-34. 203 011 IP
p54(nrb) associates with the 5' splice site within large transcription/splicing complexes.
Kameoka S, Duque P, Konarska MM
The EMBO journal (2004) 238: 1782-91. 203 011 IP
A general approach for identification of RNA-protein cross-linking sites within native human spliceosomal small nuclear ribonucleoproteins (snRNPs). Analysis of RNA-protein contacts in native U1 and U4/U6.U5 snRNPs.
Urlaub H, Hartmuth K, Kostka S, Grelle G, Lührmann R
The Journal of biological chemistry (2000) 27552: 41458-68. 203 011 IP
AU-rich elements target small nuclear RNAs as well as mRNAs for rapid degradation.
Fan XC, Myer VE, Steitz JA
Genes & development (1997) 1119: 2557-68. 203 011 IP
Evidence for a direct role of the disease modifier SCNM1 in splicing.
Howell VM, Jones JM, Bergren SK, Li L, Billi AC, Avenarius MR, Meisler MH
Human molecular genetics (2007) 1620: 2506-16. 203 011 ICC
Synergistic enhancement of production of proinflammatory cytokines of human peripheral blood monocytes by anti-Sm and anti-RNP antibodies.
Matsueda Y, Arinuma Y, Nagai T, Hirohata S
PloS one (2018) 1312: e0209282. 203 011 FACS; tested species: human
Three-dimensional structure of a pre-catalytic human spliceosomal complex B.
Boehringer D, Makarov EM, Sander B, Makarova OV, Kastner B, Lührmann R, Stark H
Nature structural & molecular biology (2004) 115: 463-8. 203 011
High intranuclear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle tracking.
Kues T, Dickmanns A, Lührmann R, Peters R, Kubitscheck U
Proceedings of the National Academy of Sciences of the United States of America (2001) 9821: 12021-6. 203 011
Detection of a novel ATP-dependent cross-linked protein at the 5' splice site-U1 small nuclear RNA duplex by methylene blue-mediated photo-cross-linking.
Liu ZR, Sargueil B, Smith CW
Molecular and cellular biology (1998) 1812: 6910-20. 203 011
Identification of a human protein that recognizes the 3' splice site during the second step of pre-mRNA splicing.
Wu S, Green MR
The EMBO journal (1997) 1614: 4421-32. 203 011
The association of the U1-specific 70K and C proteins with U1 snRNPs is mediated in part by common U snRNP proteins.
Nelissen RL, Will CL, van Venrooij WJ, Lührmann R
The EMBO journal (1994) 1317: 4113-25. 203 011
WB
ICC
IHC-P
Background
In eukaryotic cells introns are removed from pre-mRNAs by the splicesome which consists of the U1, U2, U4, U5 and U6 small nuclear ribonucleoprotein particles (snRNPs) and other proteins.
Binding of the 5´-splicing site to the U1 snRNP is one of the first steps in the spliceosome assembly. This interaction involves base-pairing between the U1 snRNA and conserved sequences spanning the 5´-splice site.
U1-70k is a member of the U1 snRNP. It has an RNA binding domain (RBD) and directly interacts with stem-loop I of U1 snRNA.