Tyrosine hydroxylase antibody - 213 211

Tyrosine hydroxylase is the rate limiting enzyme in catecholamine synthesis

Mouse monoclonal purified IgG

Cat. No.: 213 211

Amount: 100 µg

Price: $415.00

Cat. No. 213 211	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 1000 up to 1 : 10000 (see remarks) gallery IHC-P: 1 : 500 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	103E1
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the amino-terminus of rat TyrH. (UniProt Id: P04177)
Epitop	AA 65 to 163 from rat TyrH (UniProt Id: P04177)
Reactivity	Reacts with: rat (P04177), mouse (P24529). Other species not tested yet.
Remarks	WB: This antibody is less sensitive than the polyclonal antibodies (cat. no. 213 102 and 213 104. IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) can be applied to improve signal quality.
Data sheet	213_211.pdf

References for Tyrosine hydroxylase - 213 211

Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.
Vidyadhara DJ, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam TT, Sames D, Greene LE, et al.
Cell reports (2023) 423: 112231. 213 211 IHC; tested species: mouse

Cat. No.: 213 211

Amount: 100 µg

Price: $415.00

ICC

IHC

IHC-P

Background

Tyrosine hydroxylase is one of the key enzymes in the synthesis pathway of catecholamines like adrenalin, noradrenalin and dopamin and is frequently used as a marker for dopaminergic neurons. This neuronal subpopulation is especially affected in Parkinson's disease.

1 ) Vadasz C et al., Neuroscience (2007)
2 ) Rao F et al., Circulation (2007)
3 ) Lehmann IT et al., J. Biol. Chem. (2006)
4 ) Peng X et al., J. Cell. Sci. (2005)
5 ) Jeon YK et al., Mol. Cells (2001)
6 ) McLean JH et al., J. Neurosci. (1988)
7 ) Musacchio JM et al., Proc. Natl. Acad. Sci. U.S.A. (1969)

Protocols

Standard protocol

ICC

IHC

IHC-P

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