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Tyrosine hydroxylase antibody - 213 102

Tyrosine hydroxylase is the rate limiting enzyme in catecholamine synthesis
Rabbit polyclonal antiserum
Cat. No.: 213 102
Amount: 200 µl
Price: $355.00
Cat. No. 213 102 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 200 up to 1 : 2000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to residues near the amino-terminus of rat TyrH. (UniProt Id: P04177)
Reactivity Reacts with: rat (P04177), mouse (P24529).
Other species not tested yet.
Data sheet 213_102.pdf

References for Tyrosine hydroxylase - 213 102

Synaptic zinc contributes to motor and cognitive deficits in 6-hydroxydopamine mouse models of Parkinson's disease.
Sikora J, Kieffer BL, Paoletti P, Ouagazzal AM
Neurobiology of disease (2019) 134: 104681. 213 102 WB, IHC; tested species: mouse
A functional circuit formed by the autonomic nerves and myofibroblasts controls mammalian alveolar formation for gas exchange.
Zhang K, Yao E, Wang SA, Chuang E, Wong J, Minichiello L, Schroeder A, Eckalbar W, Wolters PJ, Chuang PT
Developmental cell (2022) : . 213 102 IHC; tested species: mouse
Synaptic zinc contributes to motor and cognitive deficits in 6-hydroxydopamine mouse models of Parkinson's disease.
Sikora J, Kieffer BL, Paoletti P, Ouagazzal AM
Neurobiology of disease (2019) 134: 104681. 213 102 WB, IHC; tested species: mouse
Cat. No.: 213 102
Amount: 200 µl
Price: $355.00
Synaptic zinc contributes to motor and cognitive deficits in 6-hydroxydopamine mouse models of Parkinson's disease.
Sikora J, Kieffer BL, Paoletti P, Ouagazzal AM
Neurobiology of disease (2019) 134: 104681. 213 102 WB, IHC; tested species: mouse
A functional circuit formed by the autonomic nerves and myofibroblasts controls mammalian alveolar formation for gas exchange.
Zhang K, Yao E, Wang SA, Chuang E, Wong J, Minichiello L, Schroeder A, Eckalbar W, Wolters PJ, Chuang PT
Developmental cell (2022) : . 213 102 IHC; tested species: mouse
Synaptic zinc contributes to motor and cognitive deficits in 6-hydroxydopamine mouse models of Parkinson's disease.
Sikora J, Kieffer BL, Paoletti P, Ouagazzal AM
Neurobiology of disease (2019) 134: 104681. 213 102 WB, IHC; tested species: mouse
Background
Tyrosine hydroxylase is one of the key enzymes in the synthesis pathway of catecholamines like adrenalin, noradrenalin and dopamin and is frequently used as a marker for dopaminergic neurons. This neuronal subpopulation is especially affected in Parkinson's disease.