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beta3-Tubulin antibody - 302 302

beta3-Tubulin also known as TuJ1 is a neuron specific Tubulin

 

  independently validated
  by Science Exchange initiative
Rabbit polyclonal antiserum
Cat. No.: 302 302
Amount: 200 µl
Price: $360.00
Cat. No. 302 302 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: yes
ICC: 1 : 1000 up to 1 : 5000 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse β3-tubulin (UniProt Id: Q9ERD7)
Reactivity Reacts with: human (Q13509), rat (Q4QRB4), mouse (Q9ERD7).
Other species not tested yet.
Specificity Specific for β3-tubulin.
Matching control protein/peptide 302-3P
Data sheet 302_302.pdf
Documents Science Exchange validation report

References for beta3-Tubulin - 302 302

A RhoA-mediated biomechanical response in Schwann cells modulates peripheral nerve myelination.
Seixas AI, Morais MRG, Brakebusch C, Relvas JB
Progress in neurobiology (2023) 227: 102481. 302 302 WB; tested species: mouse
Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission.
Liu GT, Kochlamazashvili G, Puchkov D, Müller R, Schultz C, Mackintosh AI, Vollweiter D, Haucke V, Soykan T
The EMBO journal (2022) : e109352. 302 302 WB; tested species: rat
Missense mutation of Fmr1 results in impaired AMPAR-mediated plasticity and socio-cognitive deficits in mice.
Prieto M, Folci A, Poupon G, Schiavi S, Buzzelli V, Pronot M, François U, Pousinha P, Lattuada N, Abelanet S, Castagnola S, et al.
Nature communications (2021) 121: 1557. 302 302 WB; tested species: mouse
Poststroke dendritic arbor regrowth requires the actin nucleator Cobl.
Ji Y, Koch D, Delgado JG, Günther M, Witte OW, Kessels MM, Frahm C, Qualmann B
PLoS biology (2021) 1912: e3001399. 302 302 WB; tested species: mouse
The Actin Nucleator Cobl Is Critical for Centriolar Positioning, Postnatal Planar Cell Polarity Refinement, and Function of the Cochlea.
Haag N, Schüler S, Nietzsche S, Hübner CA, Strenzke N, Qualmann B, Kessels MM
Cell reports (2018) 249: 2418-2431.e6. 302 302 WB; tested species: mouse
New Insights Into the Role of Cav2 Protein Family in Calcium Flux Deregulation in Fmr1-KO Neurons.
Castagnola S, Delhaye S, Folci A, Paquet A, Brau F, Duprat F, Jarjat M, Grossi M, Béal M, Martin S, Mantegazza M, et al.
Frontiers in molecular neuroscience (2018) 11: 342. 302 302 WB; tested species: mouse
Neph1 is required for neurite branching and is negatively regulated by the PRRXL1 homeodomain factor in the developing spinal cord dorsal horn.
Baltar J, Miranda RM, Cabral M, Rebelo S, Grahammer F, Huber TB, Reguenga C, Monteiro FA
Neural development (2024) 191: 13. 302 302 ICC; tested species: mouse
Essential transcription factors for induced neuron differentiation.
Lu C, Garipler G, Dai C, Roush T, Salome-Correa J, Martin A, Liscovitch-Brauer N, Mazzoni EO, Sanjana NE
Nature communications (2023) 141: 8362. 302 302 ICC; tested species: human
Generation of three induced pluripotent stem cell lines carrying different variants of the BDNF (Val66Met) polymorphism.
Horschitz S, Frank J, Koch P
Stem cell research (2023) 66: 102989. 302 302 ICC; tested species: human
Rewired glycosylation activity promotes scarless regeneration and functional recovery in spiny mice after complete spinal cord transection.
Nogueira-Rodrigues J, Leite SC, Pinto-Costa R, Sousa SC, Luz LL, Sintra MA, Oliveira R, Monteiro AC, Pinheiro GG, Vitorino M, Silva JA, et al.
Developmental cell (2022) 574: 440-450.e7. 302 302 ICC; tested species: mouse
The autophagy inducer SMER28 attenuates microtubule dynamics mediating neuroprotection.
Kirchenwitz M, Stahnke S, Grunau K, Melcher L, van Ham M, Rottner K, Steffen A, Stradal TEB
Scientific reports (2022) 121: 17805. 302 302 ICC; tested species: mouse
Essential role of the mitochondrial Na+/Ca2+ exchanger NCLX in mediating PDE2-dependent neuronal survival and learning.
Rozenfeld M, Azoulay IS, Ben Kasus Nissim T, Stavsky A, Melamed M, Stutzmann G, Hershfinkel M, Kofman O, Sekler I
Cell reports (2022) 4110: 111772. 302 302 ICC; tested species: mouse
The metabolite p-cresol impairs dendritic development, synaptogenesis, and synapse function in hippocampal neurons: Implications for autism spectrum disorder.
Guzmán-Salas S, Weber A, Malci A, Lin X, Herrera-Molina R, Cerpa W, Dorador C, Signorelli J, Zamorano P
Journal of neurochemistry (2022) 1614: 335-349. 302 302 ICC; tested species: rat
Nano-organization of spontaneous GABAergic transmission directs its autonomous function in neuronal signaling.
Guzikowski NJ, Kavalali ET
Cell reports (2022) 406: 111172. 302 302 ICC; tested species: rat
Plasmalogens regulate the AKT-ULK1 signaling pathway to control the position of the axon initial segment.
Ferreira da Silva T, Granadeiro LS, Bessa-Neto D, Luz LL, Safronov BV, Brites P
Progress in neurobiology (2021) 205: 102123. 302 302 ICC; tested species: mouse
Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 302 302 ICC; tested species: mouse
Generation and characterization of human induced pluripotent stem cells lines from four patients diagnosed with schizophrenia and one healthy control.
Stock R, Vogel S, Mau-Holzmann UA, Kriebel M, Wüst R, Fallgatter AJ, Volkmer H
Stem cell research (2020) 48: 101961. 302 302 ICC; tested species: human
Generation of oligodendrocytes and establishment of an all-human myelinating platform from human pluripotent stem cells.
García-León JA, García-Díaz B, Eggermont K, Cáceres-Palomo L, Neyrinck K, Madeiro da Costa R, Dávila JC, Baron-Van Evercooren A, Gutiérrez A, Verfaillie CM
Nature protocols (2020) : . 302 302 ICC; tested species: human
Decellularized skeletal muscles display neurotrophic effects in three-dimensional organotypic cultures.
Raffa P, Scattolini V, Gerli MFM, Perin S, Cui M, De Coppi P, Elvassore N, Caccin P, Luni C, Urciuolo A
Stem cells translational medicine (2020) : . 302 302 ICC; tested species: rat
hsa-let-7c miRNA Regulates Synaptic and Neuronal Function in Human Neurons.
McGowan H, Mirabella VR, Hamod A, Karakhanyan A, Mlynaryk N, Moore JC, Tischfield JA, Hart RP, Pang ZP
Frontiers in synaptic neuroscience (2018) 10: 19. 302 302 ICC; tested species: human
Basal glucocorticoid receptor activation induces proliferation and inhibits neuronal differentiation of human induced pluripotent stem cell-derived neuronal precursor cells.
Nürnberg E, Horschitz S, Schloss P, Meyer-Lindenberg A
The Journal of steroid biochemistry and molecular biology (2018) : . 302 302 ICC; tested species: human
Impact of preconditioning with retinoic acid during early development on morphological and functional characteristics of human induced pluripotent stem cell-derived neurons.
Horschitz S, Matthäus F, Groß A, Rosner J, Galach M, Greffrath W, Treede RD, Utikal J, Schloss P, Meyer-Lindenberg A
Stem cell research (2015) 151: 30-41. 302 302 ICC
Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes.
Kaczor P, Rakus D, Mozrzymas JW
Frontiers in cellular neuroscience (2015) 9: 120. 302 302 ICC
ARGX-119 is an agonist antibody for human MuSK that reverses disease relapse in a mouse model of congenital myasthenic syndrome.
Vanhauwaert R, Oury J, Vankerckhoven B, Steyaert C, Jensen SM, Vergoossen DLE, Kneip C, Santana L, Lim JL, Plomp JJ, Augustinus R, et al.
Science translational medicine (2024) 16765: eado7189. 302 302 IHC; tested species: mouse
Motor innervation directs the correct development of the mouse sympathetic nervous system.
Erickson AG, Motta A, Kastriti ME, Edwards S, Coulpier F, Théoulle E, Murtazina A, Poverennaya I, Wies D, Ganofsky J, Canu G, et al.
Nature communications (2024) 151: 7065. 302 302 IHC; tested species: mouse
Neuroplastin expression is essential for hearing and hair cell PMCA expression.
Lin X, Brunk MGK, Yuanxiang P, Curran AW, Zhang E, Stöber F, Goldschmidt J, Gundelfinger ED, Vollmer M, Happel MFK, Herrera-Molina R, et al.
Brain structure & function (2021) : . 302 302 IHC; tested species: mouse
Mechanism of disease and therapeutic rescue of Dok7 congenital myasthenia.
Oury J, Zhang W, Leloup N, Koide A, Corrado AD, Ketavarapu G, Hattori T, Koide S, Burden SJ
Nature (2021) : . 302 302 IHC; tested species: mouse
THC Regulates Tearing via Cannabinoid CB1 Receptors.
Thayer A, Murataeva N, Delcroix V, Wager-Miller J, Makarenkova HP, Straiker A
Investigative ophthalmology & visual science (2020) 6110: 48. 302 302 IHC; tested species: mouse
Vezatin is Required for the Maturation of the Neuromuscular Synapse.
Koppel N, Friese MB, Cardasis HL, Neubert TA, Burden SJ
Molecular biology of the cell (2019) : mbcE19060313. 302 302 IHC; tested species: mouse
BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic NeuronsIn Vivoand in Human Induced Pluripotent and Neural Stem Cells.
Jovanovic VM, Salti A, Tilleman H, Zega K, Jukic MM, Zou H, Friedel RH, Prakash N, Blaess S, Edenhofer F, Brodski C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 387: 1662-1676. 302 302 IHC; tested species: mouse
Cat. No.: 302 302
Amount: 200 µl
Price: $360.00
A RhoA-mediated biomechanical response in Schwann cells modulates peripheral nerve myelination.
Seixas AI, Morais MRG, Brakebusch C, Relvas JB
Progress in neurobiology (2023) 227: 102481. 302 302 WB; tested species: mouse
Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission.
Liu GT, Kochlamazashvili G, Puchkov D, Müller R, Schultz C, Mackintosh AI, Vollweiter D, Haucke V, Soykan T
The EMBO journal (2022) : e109352. 302 302 WB; tested species: rat
Missense mutation of Fmr1 results in impaired AMPAR-mediated plasticity and socio-cognitive deficits in mice.
Prieto M, Folci A, Poupon G, Schiavi S, Buzzelli V, Pronot M, François U, Pousinha P, Lattuada N, Abelanet S, Castagnola S, et al.
Nature communications (2021) 121: 1557. 302 302 WB; tested species: mouse
Poststroke dendritic arbor regrowth requires the actin nucleator Cobl.
Ji Y, Koch D, Delgado JG, Günther M, Witte OW, Kessels MM, Frahm C, Qualmann B
PLoS biology (2021) 1912: e3001399. 302 302 WB; tested species: mouse
The Actin Nucleator Cobl Is Critical for Centriolar Positioning, Postnatal Planar Cell Polarity Refinement, and Function of the Cochlea.
Haag N, Schüler S, Nietzsche S, Hübner CA, Strenzke N, Qualmann B, Kessels MM
Cell reports (2018) 249: 2418-2431.e6. 302 302 WB; tested species: mouse
New Insights Into the Role of Cav2 Protein Family in Calcium Flux Deregulation in Fmr1-KO Neurons.
Castagnola S, Delhaye S, Folci A, Paquet A, Brau F, Duprat F, Jarjat M, Grossi M, Béal M, Martin S, Mantegazza M, et al.
Frontiers in molecular neuroscience (2018) 11: 342. 302 302 WB; tested species: mouse
Neph1 is required for neurite branching and is negatively regulated by the PRRXL1 homeodomain factor in the developing spinal cord dorsal horn.
Baltar J, Miranda RM, Cabral M, Rebelo S, Grahammer F, Huber TB, Reguenga C, Monteiro FA
Neural development (2024) 191: 13. 302 302 ICC; tested species: mouse
Essential transcription factors for induced neuron differentiation.
Lu C, Garipler G, Dai C, Roush T, Salome-Correa J, Martin A, Liscovitch-Brauer N, Mazzoni EO, Sanjana NE
Nature communications (2023) 141: 8362. 302 302 ICC; tested species: human
Generation of three induced pluripotent stem cell lines carrying different variants of the BDNF (Val66Met) polymorphism.
Horschitz S, Frank J, Koch P
Stem cell research (2023) 66: 102989. 302 302 ICC; tested species: human
Rewired glycosylation activity promotes scarless regeneration and functional recovery in spiny mice after complete spinal cord transection.
Nogueira-Rodrigues J, Leite SC, Pinto-Costa R, Sousa SC, Luz LL, Sintra MA, Oliveira R, Monteiro AC, Pinheiro GG, Vitorino M, Silva JA, et al.
Developmental cell (2022) 574: 440-450.e7. 302 302 ICC; tested species: mouse
The autophagy inducer SMER28 attenuates microtubule dynamics mediating neuroprotection.
Kirchenwitz M, Stahnke S, Grunau K, Melcher L, van Ham M, Rottner K, Steffen A, Stradal TEB
Scientific reports (2022) 121: 17805. 302 302 ICC; tested species: mouse
Essential role of the mitochondrial Na+/Ca2+ exchanger NCLX in mediating PDE2-dependent neuronal survival and learning.
Rozenfeld M, Azoulay IS, Ben Kasus Nissim T, Stavsky A, Melamed M, Stutzmann G, Hershfinkel M, Kofman O, Sekler I
Cell reports (2022) 4110: 111772. 302 302 ICC; tested species: mouse
The metabolite p-cresol impairs dendritic development, synaptogenesis, and synapse function in hippocampal neurons: Implications for autism spectrum disorder.
Guzmán-Salas S, Weber A, Malci A, Lin X, Herrera-Molina R, Cerpa W, Dorador C, Signorelli J, Zamorano P
Journal of neurochemistry (2022) 1614: 335-349. 302 302 ICC; tested species: rat
Nano-organization of spontaneous GABAergic transmission directs its autonomous function in neuronal signaling.
Guzikowski NJ, Kavalali ET
Cell reports (2022) 406: 111172. 302 302 ICC; tested species: rat
Plasmalogens regulate the AKT-ULK1 signaling pathway to control the position of the axon initial segment.
Ferreira da Silva T, Granadeiro LS, Bessa-Neto D, Luz LL, Safronov BV, Brites P
Progress in neurobiology (2021) 205: 102123. 302 302 ICC; tested species: mouse
Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 302 302 ICC; tested species: mouse
Generation and characterization of human induced pluripotent stem cells lines from four patients diagnosed with schizophrenia and one healthy control.
Stock R, Vogel S, Mau-Holzmann UA, Kriebel M, Wüst R, Fallgatter AJ, Volkmer H
Stem cell research (2020) 48: 101961. 302 302 ICC; tested species: human
Generation of oligodendrocytes and establishment of an all-human myelinating platform from human pluripotent stem cells.
García-León JA, García-Díaz B, Eggermont K, Cáceres-Palomo L, Neyrinck K, Madeiro da Costa R, Dávila JC, Baron-Van Evercooren A, Gutiérrez A, Verfaillie CM
Nature protocols (2020) : . 302 302 ICC; tested species: human
Decellularized skeletal muscles display neurotrophic effects in three-dimensional organotypic cultures.
Raffa P, Scattolini V, Gerli MFM, Perin S, Cui M, De Coppi P, Elvassore N, Caccin P, Luni C, Urciuolo A
Stem cells translational medicine (2020) : . 302 302 ICC; tested species: rat
hsa-let-7c miRNA Regulates Synaptic and Neuronal Function in Human Neurons.
McGowan H, Mirabella VR, Hamod A, Karakhanyan A, Mlynaryk N, Moore JC, Tischfield JA, Hart RP, Pang ZP
Frontiers in synaptic neuroscience (2018) 10: 19. 302 302 ICC; tested species: human
Basal glucocorticoid receptor activation induces proliferation and inhibits neuronal differentiation of human induced pluripotent stem cell-derived neuronal precursor cells.
Nürnberg E, Horschitz S, Schloss P, Meyer-Lindenberg A
The Journal of steroid biochemistry and molecular biology (2018) : . 302 302 ICC; tested species: human
Impact of preconditioning with retinoic acid during early development on morphological and functional characteristics of human induced pluripotent stem cell-derived neurons.
Horschitz S, Matthäus F, Groß A, Rosner J, Galach M, Greffrath W, Treede RD, Utikal J, Schloss P, Meyer-Lindenberg A
Stem cell research (2015) 151: 30-41. 302 302 ICC
Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes.
Kaczor P, Rakus D, Mozrzymas JW
Frontiers in cellular neuroscience (2015) 9: 120. 302 302 ICC
ARGX-119 is an agonist antibody for human MuSK that reverses disease relapse in a mouse model of congenital myasthenic syndrome.
Vanhauwaert R, Oury J, Vankerckhoven B, Steyaert C, Jensen SM, Vergoossen DLE, Kneip C, Santana L, Lim JL, Plomp JJ, Augustinus R, et al.
Science translational medicine (2024) 16765: eado7189. 302 302 IHC; tested species: mouse
Motor innervation directs the correct development of the mouse sympathetic nervous system.
Erickson AG, Motta A, Kastriti ME, Edwards S, Coulpier F, Théoulle E, Murtazina A, Poverennaya I, Wies D, Ganofsky J, Canu G, et al.
Nature communications (2024) 151: 7065. 302 302 IHC; tested species: mouse
Neuroplastin expression is essential for hearing and hair cell PMCA expression.
Lin X, Brunk MGK, Yuanxiang P, Curran AW, Zhang E, Stöber F, Goldschmidt J, Gundelfinger ED, Vollmer M, Happel MFK, Herrera-Molina R, et al.
Brain structure & function (2021) : . 302 302 IHC; tested species: mouse
Mechanism of disease and therapeutic rescue of Dok7 congenital myasthenia.
Oury J, Zhang W, Leloup N, Koide A, Corrado AD, Ketavarapu G, Hattori T, Koide S, Burden SJ
Nature (2021) : . 302 302 IHC; tested species: mouse
THC Regulates Tearing via Cannabinoid CB1 Receptors.
Thayer A, Murataeva N, Delcroix V, Wager-Miller J, Makarenkova HP, Straiker A
Investigative ophthalmology & visual science (2020) 6110: 48. 302 302 IHC; tested species: mouse
Vezatin is Required for the Maturation of the Neuromuscular Synapse.
Koppel N, Friese MB, Cardasis HL, Neubert TA, Burden SJ
Molecular biology of the cell (2019) : mbcE19060313. 302 302 IHC; tested species: mouse
BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic NeuronsIn Vivoand in Human Induced Pluripotent and Neural Stem Cells.
Jovanovic VM, Salti A, Tilleman H, Zega K, Jukic MM, Zou H, Friedel RH, Prakash N, Blaess S, Edenhofer F, Brodski C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 387: 1662-1676. 302 302 IHC; tested species: mouse
Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape (1).
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 25 nm diameter (1).
Class III β-tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development (2).
It is widely used as a neuronal marker in normal and neoplastic tissues but has also been reported to be expressed in certain tumours of non-neuronal origin (3).