Synaptic Systems - Tomosyn2

Cat. No.: 183 203

Amount: 50 µg

Price: $375.00

Cat. No. 183 203	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 828 to 983 from mouse Tomosyn2 (UniProt Id: Q5DQR4)
Reactivity	Reacts with: rat, mouse (Q5DQR4). Other species not tested yet.
Specificity	K.O. validated PubMed: 24744148
Data sheet	183_203.pdf

References for Tomosyn2 - 183 203

Tomosyn-2 is required for normal motor performance in mice and sustains neurotransmission at motor endplates.
Geerts CJ, Plomp JJ, Koopmans B, Loos M, van der Pijl EM, van der Valk MA, Verhage M, Groffen AJ
Brain structure & function (2015) 2204: 1971-82. 183 203 WB; KO verified; tested species: mouse

Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neurons.
Subkhangulova A, Gonzalez-Lozano MA, Groffen AJA, van Weering JRT, Smit AB, Toonen RF, Verhage M
eLife (2023) 12: . 183 203 WB; KO verified; tested species: mouse

The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology.
Saldate JJ, Shiau J, Cazares VA, Stuenkel EL
The Journal of biological chemistry (2018) 2937: 2232-2246. 183 203 WB; tested species: rat

Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains.
Geerts CJ, Mancini R, Chen N, Koopmans FTW, Li KW, Smit AB, van Weering JRT, Verhage M, Groffen AJA
PloS one (2017) 127: e0180912. 183 203 WB, ICC; tested species: mouse

Cat. No.: 183 203

Amount: 50 µg

Price: $375.00

Background

SNARE proteins play crucial roles in vesicle transport by catalyzing membrane fusion events. Several proteins like the Munc 18s and tomosyn 1 (syntaxin 1A binding protein 5) interact with the neuronal plasmalemma located SNARE protein syntaxin 1a and modulate neurotransmitter release at synaptic nerve terminals.
Tomosyn 1 contains a C-terminal synaptobrevin-like R-SNARE motif that can form a stable ternary complex with syntaxin 1A and SNAP 25.
Another isoform, tomosyn 2 (syntaxin 1A binding protein 5 like), has also been described.

1 ) Gracheva EO et al., J. Neurosci. (2007)
2 ) Groffen AJ et al., J. Neurochem. (2005)
3 ) Pobbati AV et al., J. Biol. Chem. (2004)
4 ) Yizhar O et al., Proc. Natl. Acad. Sci. U.S.A. (2004)
5 ) Fujita Y et al., Neuron (1998)

Protocols

Tomosyn2 antibody - 183 203 K.O.

References for Tomosyn2 - 183 203