Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

Tomosyn1 antibody - 183 103 K.O.

Tomosyns are syntaxin 1 binding partners
Rabbit polyclonal purified antibody
Cat. No.: 183 103
Amount: 50 µg
Price: $375.00
Cat. No. 183 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to AA 561 to 774 from mouse Tomosyn1 (UniProt Id: Q8K400)
Reactivity Reacts with: rat (Q9WU70), mouse (Q8K400).
Other species not tested yet.
Specificity K.O. validated PubMed: 24744148
Data sheet 183_103.pdf

References for Tomosyn1 - 183 103

Tomosyn-2 is required for normal motor performance in mice and sustains neurotransmission at motor endplates.
Geerts CJ, Plomp JJ, Koopmans B, Loos M, van der Pijl EM, van der Valk MA, Verhage M, Groffen AJ
Brain structure & function (2015) 2204: 1971-82. 183 103 WB; KO verified
Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to increase insulin secretion from pancreatic β-cells improving glucose clearance.
Rahman MM, Pathak A, Schueler KL, Alsharif H, Michl A, Alexander J, Kim JA, Bhatnagar S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2023) 378: e23075. 183 103 WB, ICC; tested species: mouse
Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neurons.
Subkhangulova A, Gonzalez-Lozano MA, Groffen AJA, van Weering JRT, Smit AB, Toonen RF, Verhage M
eLife (2023) 12: . 183 103 WB, ICC; KO verified; tested species: mouse
Tomosyn regulates the small RhoA GTPase to control the dendritic stability of neurons and the surface expression of AMPA receptors.
Shen W, Kilander MBC, Bridi MS, Frei JA, Niescier RF, Huang S, Lin YC
Journal of neuroscience research (2020) : . 183 103 WB, IP, ICC; tested species: mouse
Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 183 103 WB; tested species: mouse
The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology.
Saldate JJ, Shiau J, Cazares VA, Stuenkel EL
The Journal of biological chemistry (2018) 2937: 2232-2246. 183 103 WB, IP, ICC; KD verified; tested species: rat
Dynamic Partitioning of Synaptic Vesicle Pools by the SNARE-Binding Protein Tomosyn.
Cazares VA, Njus MM, Manly A, Saldate JJ, Subramani A, Ben-Simon Y, Sutton MA, Ashery U, Stuenkel EL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3644: 11208-11222. 183 103 WB, ICC; KD verified; tested species: rat
Tomosyn regulates the small RhoA GTPase to control the dendritic stability of neurons and the surface expression of AMPA receptors.
Shen W, Kilander MBC, Bridi MS, Frei JA, Niescier RF, Huang S, Lin YC
Journal of neuroscience research (2020) : . 183 103 WB, IP, ICC; tested species: mouse
The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology.
Saldate JJ, Shiau J, Cazares VA, Stuenkel EL
The Journal of biological chemistry (2018) 2937: 2232-2246. 183 103 WB, IP, ICC; KD verified; tested species: rat
Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains.
Geerts CJ, Mancini R, Chen N, Koopmans FTW, Li KW, Smit AB, van Weering JRT, Verhage M, Groffen AJA
PloS one (2017) 127: e0180912. 183 103 IP, ICC, EM; KO verified; tested species: mouse
Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to increase insulin secretion from pancreatic β-cells improving glucose clearance.
Rahman MM, Pathak A, Schueler KL, Alsharif H, Michl A, Alexander J, Kim JA, Bhatnagar S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2023) 378: e23075. 183 103 WB, ICC; tested species: mouse
Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons.
van Bommel DM, Toonen RF, Verhage M
Scientific reports (2023) 131: 2871. 183 103 ICC; tested species: mouse
Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neurons.
Subkhangulova A, Gonzalez-Lozano MA, Groffen AJA, van Weering JRT, Smit AB, Toonen RF, Verhage M
eLife (2023) 12: . 183 103 WB, ICC; KO verified; tested species: mouse
Tomosyn regulates the small RhoA GTPase to control the dendritic stability of neurons and the surface expression of AMPA receptors.
Shen W, Kilander MBC, Bridi MS, Frei JA, Niescier RF, Huang S, Lin YC
Journal of neuroscience research (2020) : . 183 103 WB, IP, ICC; tested species: mouse
The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology.
Saldate JJ, Shiau J, Cazares VA, Stuenkel EL
The Journal of biological chemistry (2018) 2937: 2232-2246. 183 103 WB, IP, ICC; KD verified; tested species: rat
Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains.
Geerts CJ, Mancini R, Chen N, Koopmans FTW, Li KW, Smit AB, van Weering JRT, Verhage M, Groffen AJA
PloS one (2017) 127: e0180912. 183 103 IP, ICC, EM; KO verified; tested species: mouse
Dynamic Partitioning of Synaptic Vesicle Pools by the SNARE-Binding Protein Tomosyn.
Cazares VA, Njus MM, Manly A, Saldate JJ, Subramani A, Ben-Simon Y, Sutton MA, Ashery U, Stuenkel EL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3644: 11208-11222. 183 103 WB, ICC; KD verified; tested species: rat
Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains.
Geerts CJ, Mancini R, Chen N, Koopmans FTW, Li KW, Smit AB, van Weering JRT, Verhage M, Groffen AJA
PloS one (2017) 127: e0180912. 183 103 IP, ICC, EM; KO verified; tested species: mouse
Cat. No.: 183 103
Amount: 50 µg
Price: $375.00
Tomosyn-2 is required for normal motor performance in mice and sustains neurotransmission at motor endplates.
Geerts CJ, Plomp JJ, Koopmans B, Loos M, van der Pijl EM, van der Valk MA, Verhage M, Groffen AJ
Brain structure & function (2015) 2204: 1971-82. 183 103 WB; KO verified
Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to increase insulin secretion from pancreatic β-cells improving glucose clearance.
Rahman MM, Pathak A, Schueler KL, Alsharif H, Michl A, Alexander J, Kim JA, Bhatnagar S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2023) 378: e23075. 183 103 WB, ICC; tested species: mouse
Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neurons.
Subkhangulova A, Gonzalez-Lozano MA, Groffen AJA, van Weering JRT, Smit AB, Toonen RF, Verhage M
eLife (2023) 12: . 183 103 WB, ICC; KO verified; tested species: mouse
Tomosyn regulates the small RhoA GTPase to control the dendritic stability of neurons and the surface expression of AMPA receptors.
Shen W, Kilander MBC, Bridi MS, Frei JA, Niescier RF, Huang S, Lin YC
Journal of neuroscience research (2020) : . 183 103 WB, IP, ICC; tested species: mouse
Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 183 103 WB; tested species: mouse
The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology.
Saldate JJ, Shiau J, Cazares VA, Stuenkel EL
The Journal of biological chemistry (2018) 2937: 2232-2246. 183 103 WB, IP, ICC; KD verified; tested species: rat
Dynamic Partitioning of Synaptic Vesicle Pools by the SNARE-Binding Protein Tomosyn.
Cazares VA, Njus MM, Manly A, Saldate JJ, Subramani A, Ben-Simon Y, Sutton MA, Ashery U, Stuenkel EL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3644: 11208-11222. 183 103 WB, ICC; KD verified; tested species: rat
Tomosyn regulates the small RhoA GTPase to control the dendritic stability of neurons and the surface expression of AMPA receptors.
Shen W, Kilander MBC, Bridi MS, Frei JA, Niescier RF, Huang S, Lin YC
Journal of neuroscience research (2020) : . 183 103 WB, IP, ICC; tested species: mouse
The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology.
Saldate JJ, Shiau J, Cazares VA, Stuenkel EL
The Journal of biological chemistry (2018) 2937: 2232-2246. 183 103 WB, IP, ICC; KD verified; tested species: rat
Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains.
Geerts CJ, Mancini R, Chen N, Koopmans FTW, Li KW, Smit AB, van Weering JRT, Verhage M, Groffen AJA
PloS one (2017) 127: e0180912. 183 103 IP, ICC, EM; KO verified; tested species: mouse
Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to increase insulin secretion from pancreatic β-cells improving glucose clearance.
Rahman MM, Pathak A, Schueler KL, Alsharif H, Michl A, Alexander J, Kim JA, Bhatnagar S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2023) 378: e23075. 183 103 WB, ICC; tested species: mouse
Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons.
van Bommel DM, Toonen RF, Verhage M
Scientific reports (2023) 131: 2871. 183 103 ICC; tested species: mouse
Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neurons.
Subkhangulova A, Gonzalez-Lozano MA, Groffen AJA, van Weering JRT, Smit AB, Toonen RF, Verhage M
eLife (2023) 12: . 183 103 WB, ICC; KO verified; tested species: mouse
Tomosyn regulates the small RhoA GTPase to control the dendritic stability of neurons and the surface expression of AMPA receptors.
Shen W, Kilander MBC, Bridi MS, Frei JA, Niescier RF, Huang S, Lin YC
Journal of neuroscience research (2020) : . 183 103 WB, IP, ICC; tested species: mouse
The ubiquitin-proteasome system functionally links neuronal Tomosyn-1 to dendritic morphology.
Saldate JJ, Shiau J, Cazares VA, Stuenkel EL
The Journal of biological chemistry (2018) 2937: 2232-2246. 183 103 WB, IP, ICC; KD verified; tested species: rat
Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains.
Geerts CJ, Mancini R, Chen N, Koopmans FTW, Li KW, Smit AB, van Weering JRT, Verhage M, Groffen AJA
PloS one (2017) 127: e0180912. 183 103 IP, ICC, EM; KO verified; tested species: mouse
Dynamic Partitioning of Synaptic Vesicle Pools by the SNARE-Binding Protein Tomosyn.
Cazares VA, Njus MM, Manly A, Saldate JJ, Subramani A, Ben-Simon Y, Sutton MA, Ashery U, Stuenkel EL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3644: 11208-11222. 183 103 WB, ICC; KD verified; tested species: rat
Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains.
Geerts CJ, Mancini R, Chen N, Koopmans FTW, Li KW, Smit AB, van Weering JRT, Verhage M, Groffen AJA
PloS one (2017) 127: e0180912. 183 103 IP, ICC, EM; KO verified; tested species: mouse
Background

SNARE proteins play crucial roles in vesicle transport by catalyzing membrane fusion events. Several proteins like the Munc 18s and tomosyn 1 (syntaxin 1A binding protein 5) interact with the neuronal plasmalemma located SNARE protein syntaxin 1a and modulate neurotransmitter release at synaptic nerve terminals.
Tomosyn 1 contains a C-terminal synaptobrevin-like R-SNARE motif that can form a stable ternary complex with syntaxin 1A and SNAP 25.
Another isoform, tomosyn 2 (syntaxin 1A binding protein 5 like), has also been described.