Tomosyn1/2 antibody - 183 003

Tomosyns are syntaxin 1 binding partners

Rabbit polyclonal purified antibody

Cat. No.: 183 003

Amount: 50 µg

Price: $375.00

Cat. No. 183 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1067 to 1139 from rat Tomosyn1 (UniProt Id: Q9WU70)
Reactivity	Reacts with: human (Q5T5C0, Q9Y2K9), rat (Q9WU70), mouse (Q8K400, Q5DQR4), cow. Other species not tested yet.
Specificity	Cross-reacts with tomosyn 2 due to sequence homology.
Matching control protein/peptide	183-0P
Data sheet	183_003.pdf

References for Tomosyn1/2 - 183 003

SNARE protein recycling by αSNAP and βSNAP supports synaptic vesicle priming.
Burgalossi A, Jung S, Meyer G, Jockusch WJ, Jahn O, Taschenberger H, O'Connor VM, Nishiki T, Takahashi M, Brose N, Rhee JS, et al.
Neuron (2010) 683: 473-87. 183 003 WB

Cat. No.: 183 003

Amount: 50 µg

Price: $375.00

ICC

IHC-P

Background

SNARE proteins play crucial roles in vesicle transport by catalyzing membrane fusion events. Several proteins like the Munc 18s and tomosyn 1 (syntaxin 1A binding protein 5) interact with the neuronal plasmalemma located SNARE protein syntaxin 1a and modulate neurotransmitter release at synaptic nerve terminals.
Tomosyn 1 contains a C-terminal synaptobrevin-like R-SNARE motif that can form a stable ternary complex with syntaxin 1A and SNAP 25.
Another isoform, tomosyn 2 (syntaxin 1A binding protein 5 like), has also been described.

1 ) Gracheva EO et al., J. Neurosci. (2007)
2 ) Groffen AJ et al., J. Neurochem. (2005)
3 ) Pobbati AV et al., J. Biol. Chem. (2004)
4 ) Yizhar O et al., Proc. Natl. Acad. Sci. U.S.A. (2004)
5 ) Fujita Y et al., Neuron (1998)

Protocols

ICC

IHC-P

OTHER

Preabsorbtion

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