Cat. No.: 183-0P
Amount: 100 µg
Price:
$105.00
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| Cat. No. 183-0P |
100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
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| Immunogen | Recombinant protein corresponding to AA 1067 to 1139 from rat Tomosyn1 (UniProt Id: Q9WU70) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 183 003 |
| Remarks |
This control protein consists of the recombinant protein (aa 1031 - 1103 of rat tomosyn 1) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 183-0p.pdf |
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SNARE proteins play crucial roles in vesicle transport by catalyzing membrane fusion events. Several proteins like the Munc 18s and tomosyn 1 (syntaxin 1A binding protein 5) interact with the neuronal plasmalemma located SNARE protein syntaxin 1a and modulate neurotransmitter release at synaptic nerve terminals.
Tomosyn 1 contains a C-terminal synaptobrevin-like R-SNARE motif that can form a stable ternary complex with syntaxin 1A and SNAP 25.
Another isoform, tomosyn 2 (syntaxin 1A binding protein 5 like), has also been described.