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TNiK antibody - 290 003

TNiK is a kinase involved in the regulation of the cytoskeleton
Rabbit polyclonal purified antibody
Cat. No.: 290 003
Amount: 50 µg
Price: $375.00
Cat. No. 290 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to AA 601 to 704 from mouse TNiK (UniProt Id: P83510)
Reactivity Reacts with: rat, mouse (P83510).
Other species not tested yet.
Data sheet 290_003.pdf
Cat. No.: 290 003
Amount: 50 µg
Price: $375.00
Background
Traf2- and Nck-interacting kinase (TNiK) is mainly expressed in muscle and brain. Upon overexpression it activates the c-Jun N-terminal kinase (JNK) pathway and leads to disruption of F-actin structure.
In neurons TNiK has been shown to be postsynaptically enriched in dendrites, where it is involved in Rap2 mediated signal transduction regulating neuronal structure and GluA receptor function.