Cat. No.: 502-P2
Amount: 100 µg
Price:
$280.00
|
|
|
| Cat. No. 502-P2 |
100 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 50 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use. |
| Applications |
|
| Loading/lane | 10-30 µg |
| Source | Rat, White Wistar |
| Remarks |
Since several synaptic proteins aggregate after boiling, these lysates are unboiled. Boil sample before loading, if this is recommended for your protocol. |
| Data sheet | 502-p2.pdf |
| Documents | MSDS |
|
|
In many cases the relative concentration of the protein of interest is high enough to use whole brain lysate for Western blot testing. If the concentration is less than 0.2% of total protein it may be difficult to distinguish signals from background noise. Since a lot of proteins are localized in distinct cellular compartments, subfractioning may overcome this problem. This fraction enriched for synaptosomes (P2) been prepared according to our protocol. Protein concentration has been determined with a Bradford protein quantification assay.