Cat. No.: 501-TOT
Amount: 200 µg
Price:
$280.00
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| Cat. No. 501-TOT |
200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 100 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use. |
| Applications |
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| Loading/lane | 10-30 µg |
| Source | Rat, White Wistar |
| Remarks |
Since several synaptic proteins aggregate after boiling, these lysates are unboiled. Boil sample before loading, if this is recommended for your protocol. |
| Data sheet | 501-tot.pdf |
| Documents | MSDS |
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In many cases the relative concentration of the protein of interest is high enough to use whole brain lysate for Western blot testing. If the concentration is less than 0.2% of total protein it may be difficult to distinguish signals from background noise. Since a lot of proteins are localized in distinct cellular compartments, subfractioning may overcome this problem. Fractions have been prepared according to our protocol. Protein concentration has been determined with a Bradford protein quantification assay.