Neuroligin WT lysate - 510-Nlg-WT

Ready to use tissue lysates for western blots

Mouse tissue lysate

Cat. No.: 510-Nlg-WT

Amount: 200 µg

Price: $280.00

Cat. No. 510-Nlg-WT	200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 100 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use.
Applications	WB: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.
Loading/lane	10-30 µg
Source	Mouse, Balb-C
Remarks	WB: Since several synaptic proteins aggregate after boiling, these lysates are unboiled. Boil sample before loading, if this is recommended for your protocol.
Data sheet	510-nlg-wt.pdf
Documents	MSDS

Cat. No.: 510-Nlg-WT

Amount: 200 µg

Price: $280.00

Background

These total brain lysates from wild type and Neuroligin 1-4 KO strains can be used to investigate antibody specificity in western blot experiments. Protein concentration has been determined with a Bradford protein quantification assay.

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6 ) Missler M et al., J. Neurochem. (1998)
7 ) Ichtchenko K et al., J. Biol. Chem. (1996)
8 ) Ichtchenko K et al., Cell (1995)
9 ) Südhof TC et al., Nature (1995)

Protocols