Spinal cord lysate - 505-MSC

Ready to use tissue lysates for western blots

Mouse tissue lysate

Cat. No.: 505-MSC

Amount: 200 µg

Price: $280.00

Cat. No. 505-MSC	200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 100 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use.
Applications	WB: yes gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.
Loading/lane	10-30 µg
Source	Mouse, Balb-C
Remarks	Since several synaptic proteins aggregate after boiling, these lysates are unboiled. Boil sample before loading, if this is recommended for your protocol.
Data sheet	505-msc.pdf
Documents	MSDS

Cat. No.: 505-MSC

Amount: 200 µg

Price: $280.00

Background

In many cases the relative concentration of the protein of interest is high enough to use whole tissue lysate for Western blot testing. If the concentration is less than 0.2% of total protein it may be difficult to distinguish signals from background noise. Since a lot of proteins are localized in distinct cellular compartments, subfractioning may overcome this problem. Protein concentration has been determined with a Bradford protein quantification assay.

1 ) Huttner WB et al., J. Cell Biol. (1983)
2 ) Nagy A et al., Brain Res. (1976)

Protocols