Synaptic Systems - Taok2 beta

Cat. No.: 395 003

Amount: 50 µg

Price: $375.00

Cat. No. 395 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes gallery ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1020 to 1041 from mouse Taok2-2 (UniProt Id: Q6ZQ29-2)
Reactivity	Reacts with: mouse (Q6ZQ29-2), rat (Q9JLS3-2). Other species not tested yet.
Specificity	K.O. validated PubMed: 36417873
Data sheet	395_003.pdf

References for Taok2 beta - 395 003

The autism susceptibility kinase, TAOK2, phosphorylates eEF2 and modulates translation.
Henis M, Rücker T, Scharrenberg R, Richter M, Baltussen L, Hong S, Meka DP, Schwanke B, Neelagandan N, Daaboul D, Murtaza N, et al.
Science advances (2024) 1015: eadf7001. 395 003 WB, ICC, IP; tested species: mouse

Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies.
Murtaza N, Cheng AA, Brown CO, Meka DP, Hong S, Uy JA, El-Hajjar J, Pipko N, Unda BK, Schwanke B, Xing S, et al.
Cell reports (2022) 418: 111678. 395 003 WB, IP; KO verified; tested species: mouse,human

Cat. No.: 395 003

Amount: 50 µg

Price: $375.00

Background

The serine/threonine-protein kinase Taok 2 or TAO 2 plays a role in membrane blebbing and the formation of apoptotic bodies. Taok 2 is involved in the MAPK14/p38 MAPK stress-activated MAPK cascade. It may also affect the cytoskeletal organization and stability, the dendritic formation, and the osmotic stress-MAPK8 pathway. There are two isoforms, Taok 2α and Taok 2β, only the latter is required for PCDH8 endocytosis and CDH2 cointernalization.

1 ) Yadav S et al., Neuron (2017)
2 ) de Anda FC et al., Nat. Neurosci. (2012)
3 ) Wojtala RL et al., J. Biol. Chem. (2011)
4 ) Chen Z et al., J. Biol. Chem. (2001)
5 ) Chen Z et al., J. Biol. Chem. (1999)

Protocols

Standard protocol

Other products for this target

Taok2 beta antibody - 395 003 K.O.

References for Taok2 beta - 395 003