Synaptic Systems - alpha/beta Synuclein

Cat. No.: 128 111BT

Amount: 100 µg

Price: $465.00

IHC

IHC-P

Background

Synuclein proteins are produced by three genes. They share structural resemblance to apolipoproteins, but are abundant in the neuronal cytosol and present in enriched amounts at presynaptic terminals.
Synucleins have been specifically implicated in three diseases: Alzheimer's (AD), Parkinson's (PD) and breast cancer. In AD, a peptide derived from α-synuclein forms an intrinsic component of plaque amyloid. In PD, an α-synuclein allele is genetically linked to several independent familial cases, and the protein appears to accumulate in Lewy bodies. In breast cancer, increased expression of γ-synuclein correlates with disease progression.
In songbirds, α-synuclein expression is correlated with plasticity in the developing song control system. Although the normal function of synucleins is unknown, a role in synaptic plasticity seems likely.

1 ) Polymeropoulos MH et al., Ann. N. Y. Acad. Sci. (2000)
2 ) Stefanova N et al., J. Neural Transm. Suppl. (2000)
3 ) Clayton DF et al., Trends Neurosci. (1998)
4 ) Goedert M et al., Curr. Opin. Neurobiol. (1998)
5 ) Hardy J et al., Science (1998)
6 ) Lavedan C et al., Genome Res. (1998)
7 ) Lozano AM et al., Curr. Opin. Neurobiol. (1998)

Protocols

IHC

IHC-P

Other products for this target

Cat. No. 128 111BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	56F2
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 140 from human α-Synuclein (UniProt Id: P37840)
Reactivity	Reacts with: human (P37840, Q16143), rat (P37377, Q63754), mouse (O55042, Q91ZZ3). Other species not tested yet.
Specificity	Recognizes α- and β-synuclein.
Data sheet	128_111bt.pdf

alpha/beta Synuclein antibody - 128 111BT