Synaptic Systems - Syntenin

Cat. No.: 133 003

Amount: 50 µg

Price: $375.00

Cat. No. 133 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 100 up to 1 : 1000 (AP staining) gallery IP: yes ICC: yes IHC: not tested yet IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 298 from human Syntenin1 (UniProt Id: O00560)
Reactivity	Reacts with: human (O00560, Q9H190). Weaker signal: rat (Q9JI92, Q4KLN0), mouse (O08992, Q99JZ0). Other species not tested yet.
Specificity	Detects human protein with higher sensitivity. Recognizes syntenin 1 and 2. K.D. validated PubMed: 22535526
Matching control protein/peptide	133-0P
Data sheet	133_003.pdf

References for Syntenin - 133 003

Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cells.
Valdivia A, Cárdenas A, Brenet M, Maldonado H, Kong M, Díaz J, Burridge K, Schneider P, San Martín A, García-Mata R, Quest AFG, et al.
Cell communication and signaling : CCS (2020) 181: 129. 133 003 WB; tested species: rat

Proteomic peptide phage display uncovers novel interactions of the PDZ1-2 supramodule of syntenin.
Garrido-Urbani S, Garg P, Ghossoub R, Arnold R, Lembo F, Sundell GN, Kim PM, Lopez M, Zimmermann P, Sidhu SS, Ivarsson Y, et al.
FEBS letters (2016) 5901: 3-12. 133 003 WB

NG2 regulates directional migration of oligodendrocyte precursor cells via Rho GTPases and polarity complex proteins.
Binamé F, Sakry D, Dimou L, Jolivel V, Trotter J
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3326: 10858-74. 133 003 WB

Association of syntenin-1 with M-RIP polarizes Rac-1 activation during chemotaxis and immune interactions.
Sala-Valdés M, Gordón-Alonso M, Tejera E, Ibáñez A, Cabrero JR, Ursa A, Mittelbrunn M, Lozano F, Sánchez-Madrid F, Yáñez-Mó M
Journal of cell science (2012) 125Pt 5: 1235-46. 133 003 WB, ICC

The PDZ-adaptor protein syntenin-1 regulates HIV-1 entry.
Gordón-Alonso M, Rocha-Perugini V, Álvarez S, Moreno-Gonzalo O, Ursa A, López-Martín S, Izquierdo-Useros N, Martínez-Picado J, Muñoz-Fernández MÁ, Yáñez-Mó M, Sánchez-Madrid F, et al.
Molecular biology of the cell (2012) 2312: 2253-63. 133 003 WB, ICC, IP; KD verified; tested species: human

Tyrosine dephosphorylation of the syndecan-1 PDZ binding domain regulates syntenin-1 recruitment.
Sulka B, Lortat-Jacob H, Terreux R, Letourneur F, Rousselle P
The Journal of biological chemistry (2009) 28416: 10659-71. 133 003 WB, ICC

The tandem PDZ protein Syntenin interacts with the aminoacyl tRNA synthetase complex in a lysyl-tRNA synthetase-dependent manner.
Meerschaert K, Remue E, De Ganck A, Staes A, Boucherie C, Gevaert K, Vandekerckhove J, Kleiman L, Gettemans J
Journal of proteome research (2008) 711: 4962-73. 133 003 WB, IP

Stimulated release of intraluminal vesicles from Weibel-Palade bodies.
Streetley J, Fonseca AV, Turner J, Kiskin NI, Knipe L, Rosenthal PB, Carter T
Blood (2019) 13325: 2707-2717. 133 003 ICC; tested species: human

Cat. No.: 133 003

Amount: 50 µg

Price: $375.00

IHC-P

Background

Syntenin is an ubiquitously expressed PDZ domain protein that binds to the cytoplasmic tails of several cell surface proteins. It has been particularly implicated in cell-cell interactions in the brain via its interactions with proteins such as syndecans, ephrins and neurofascin.

1 ) Grootjans JJ et al., J. Biol. Chem. (2000)
2 ) Fernández-Larrea J et al., Mol. Cell (1999)
3 ) Fialka I et al., J. Biol. Chem. (1999)
4 ) Grootjans JJ et al., Proc. Natl. Acad. Sci. U.S.A. (1997)

Protocols

Standard protocol

ICC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Syntenin antibody - 133 003 K.D.

References for Syntenin - 133 003