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Syntaxin11 antibody - 110 113 K.O.

Syntaxi11 is a SNARE protein abundantly expressed in the immune system
Rabbit polyclonal purified antibody
Cat. No.: 110 113
Amount: 50 µg
Price: $375.00
Cat. No. 110 113 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 gallery  
IHC: not tested yet
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide corresponding to AA 1 to 15 from mouse Syntaxin11 (UniProt Id: Q9D3G5)
Reactivity Reacts with: rat (A0A0G2K516), mouse (Q9D3G5), human (O75558).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 110-11P
Data sheet 110_113.pdf

References for Syntaxin11 - 110 113

Dynamic cycling of t-SNARE acylation regulates platelet exocytosis.
Zhang J, Huang Y, Chen J, Zhu H, Whiteheart SW
The Journal of biological chemistry (2018) 29310: 3593-3606. 110 113 WB; tested species: human
VAMP-7 links granule exocytosis to actin reorganization during platelet activation.
Koseoglu S, Peters CG, Fitch-Tewfik JL, Aisiku O, Danglot L, Galli T, Flaumenhaft R
Blood (2015) 1265: 651-60. 110 113 WB
Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability.
Golebiewska EM, Harper MT, Williams CM, Savage JS, Goggs R, Fischer von Mollard G, Poole AW
The Journal of biological chemistry (2015) 2903: 1536-45. 110 113 WB
Syntaxin-11, but not syntaxin-2 or syntaxin-4, is required for platelet secretion.
Ye S, Karim ZA, Al Hawas R, Pessin JE, Filipovich AH, Whiteheart SW
Blood (2012) 12012: 2484-92. 110 113 WB
Cat. No.: 110 113
Amount: 50 µg
Price: $375.00
Dynamic cycling of t-SNARE acylation regulates platelet exocytosis.
Zhang J, Huang Y, Chen J, Zhu H, Whiteheart SW
The Journal of biological chemistry (2018) 29310: 3593-3606. 110 113 WB; tested species: human
VAMP-7 links granule exocytosis to actin reorganization during platelet activation.
Koseoglu S, Peters CG, Fitch-Tewfik JL, Aisiku O, Danglot L, Galli T, Flaumenhaft R
Blood (2015) 1265: 651-60. 110 113 WB
Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability.
Golebiewska EM, Harper MT, Williams CM, Savage JS, Goggs R, Fischer von Mollard G, Poole AW
The Journal of biological chemistry (2015) 2903: 1536-45. 110 113 WB
Syntaxin-11, but not syntaxin-2 or syntaxin-4, is required for platelet secretion.
Ye S, Karim ZA, Al Hawas R, Pessin JE, Filipovich AH, Whiteheart SW
Blood (2012) 12012: 2484-92. 110 113 WB
Background

Syntaxin 11, a member of the SNARE family of proteins, localizes to late endosomes and the trans-Golgi network and has been shown to interact with VAMP proteins and SNAP 23. Unlike other syntaxins it does not possess a carboxy-terminal transmembrane anchor.
In the immune systems several cell types like phagocytic immune cells, resting natural killer (NK) cells and CD8-T-cells express syntaxin 11. High levels of protein can also be found in lung and placenta but not in brain where it is almost undetectable.