Cat. No. 110 111 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: yes (see remarks) Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 200 up to 1 : 1000 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 (see remarks) gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 500 gallery In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.', $event)" style="cursor: help;">DNA-PAINT: yes Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.', $event)" style="cursor: help;">EM: yes |
Clone | 78.3 |
Subtype | IgG2a (κ light chain) |
Immunogen | Recombinant protein corresponding to cytoplasmic-domain without membrane anchor of rat Syntaxin 1A. (UniProt Id: P32851) |
Reactivity |
Reacts with: human (Q16623), rat (P32851), mouse (O35526), mammals, chicken. Other species not tested yet. |
Specificity | Specific for syntaxin 1A, no cross-reactivity to syntaxin 1B. K.O. validated PubMed: 38512129 |
Matching control protein/peptide | 110-1P |
Remarks |
IP: Immunoprecipitates syntaxin1A including complexes with synaptobrevin and SNAP25. |
Data sheet | 110_111.pdf |
Syntaxin 1, also known as p35, is a small integral membrane protein that is abundantly expressed in neurons and neuroendocrine cells. It was initially discovered as HPC-1. Syntaxin 1 is an essential component of the exocytotic fusion machine and interacts with several other proteins important for synaptic function, including its partners in the fusion complex synaptobrevin, SNAP 25, α-SNAP, synaptotagmin 1, Munc 18/n-Sec1 and Ca2+-channels.
Syntaxin 1 is localized primarily to the neuronal plasmalemma and is concentrated in synapses where pools of the protein are also present on recycling organelles including synaptic vesicles. It is the main target of one of the Botulinum neurotoxins BoNT/C1 which, however, cannot cleave the protein when complexed with its partner proteins in the fusion complex.