Synip antibody - 350 003

Synip is a syntaxin 4 binding partner

Rabbit polyclonal purified antibody

Cat. No.: 350 003

Amount: 50 µg

Price: $375.00

Cat. No. 350 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 265 to 557 from mouse Synip (UniProt Id: Q9WV89)
Reactivity	Reacts with: rat, mouse (Q9WV89). Other species not tested yet.
Data sheet	350_003.pdf

Cat. No.: 350 003

Amount: 50 µg

Price: $375.00

Background

Synip, also referred to as Stxbp4, is a syntaxin 4 binding protein that is anchored to the plasma membrane by binding to phosphatidylinositol (3,4,5)-triphosphate. It is involved in the insulin regulated glucose transport mediated by Glut 4.

1 ) Yamada E et al., Endocr. J. (2014)
2 ) Yu H et al., J. Biol. Chem. (2013)
3 ) Saito T et al., PLoS ONE (2012)
4 ) Yamada E et al., J. Cell Biol. (2005)
5 ) Saito T et al., J. Biol. Chem. (2003)
6 ) Min J et al., Mol. Cell (1999)

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