Synaptic Systems - Syncollin

Cat. No.: 225 011

Amount: 100 µg

Price: $415.00

Cat. No. 225 011	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	87.1
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 22 to 134 from rat Syncollin (UniProt Id: O35775)
Reactivity	Reacts with: rat (O35775), mouse (Q8VCK7). Other species not tested yet.
Data sheet	225_011.pdf

References for Syncollin - 225 011

The secretory granule protein syncollin localizes to HL-60 cells and neutrophils.
Bach JP, Borta H, Ackermann W, Faust F, Borchers O, Schrader M
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2006) 548: 877-88. 225 011 WB; tested species: rat

Interaction of syncollin with GP-2, the major membrane protein of pancreatic zymogen granules, and association with lipid microdomains.
Kalus I, Hodel A, Koch A, Kleene R, Edwardson JM, Schrader M
The Biochemical journal (2002) 362Pt 2: 433-42. 225 011 WB

Loss of the zymogen granule protein syncollin affects pancreatic protein synthesis and transport but not secretion.
Antonin W, Wagner M, Riedel D, Brose N, Jahn R
Molecular and cellular biology (2002) 225: 1545-54. 225 011 WB; KO verified; tested species: mouse

Analysis of the association of syncollin with the membrane of the pancreatic zymogen granule.
An SJ, Hansen NJ, Hodel A, Jahn R, Edwardson JM
The Journal of biological chemistry (2000) 27515: 11306-11. 225 011 WB

Cat. No.: 225 011

Amount: 100 µg

Price: $415.00

Background

The exocrine pancreas is specialized for the synthesis and secretion of digestive enzymes which are stored in zymogen granules. Syncollin is predominantly associated with the lumenal membrane surface of these organelles. It forms oligomers and can bind to to syntaxin 1 and syntaxin 2 in a calcium dependent manner. The exact physiological role has not yet been elucidated.

1 ) Bach JP et al., J. Histochem. Cytochem. (2006)
2 ) Kalus I et al., Biochem. J. (2002)
3 ) Antonin W et al., Mol. Cell. Biol. (2002)
4 ) An SJ et al., J. Biol. Chem. (2000)

Protocols

Syncollin antibody - 225 011

References for Syncollin - 225 011