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Home > Products > 106 211

Synapsin2 antibody - 106 211 K.O.

Synapsins are peripheral synaptic vesicle proteins and substrate for several protein kinases
Mouse monoclonal purified IgG
Cat. No.: 106 211
Amount: 100 µg
Price: $415.00
Cat. No. 106 211 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 200 gallery  
EM: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Clone 27E3
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues surrounding (AA 450) of rat Synapsin2 (UniProt Id: Q63537-1)
Reactivity Reacts with: human (Q92777), rat (Q63537), mouse (Q64332).
No signal: zebrafish.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 106-2P
Remarks

WB: This antibody is less sensitive than the polyclonal rabbit antibody (cat. no. 106 203). Enriched synaptic vesicles are recommended for westernblotting.
Data sheet 106_211.pdf

References for Synapsin2 - 106 211

MicroRNA-153 impairs hippocampal synaptic vesicle trafficking via downregulation of synapsin I in rats following chronic cerebral hypoperfusion.
Zhang S, Yan ML, Yang L, An XB, Zhao HM, Xia SN, Jin Z, Huang SY, Qu Y, Ai J
Experimental neurology (2020) : 113389. 106 211 WB; tested species: rat
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 106 211 WB
Short-term plasticity at cerebellar granule cell to molecular layer interneuron synapses expands information processing.
Dorgans K, Demais V, Bailly Y, Poulain B, Isope P, Doussau F
eLife (2019) 8: . 106 211 IHC, EM; tested species: mouse
Short-term plasticity at cerebellar granule cell to molecular layer interneuron synapses expands information processing.
Dorgans K, Demais V, Bailly Y, Poulain B, Isope P, Doussau F
eLife (2019) 8: . 106 211 IHC, EM; tested species: mouse
Cat. No.: 106 211
Amount: 100 µg
Price: $415.00
MicroRNA-153 impairs hippocampal synaptic vesicle trafficking via downregulation of synapsin I in rats following chronic cerebral hypoperfusion.
Zhang S, Yan ML, Yang L, An XB, Zhao HM, Xia SN, Jin Z, Huang SY, Qu Y, Ai J
Experimental neurology (2020) : 113389. 106 211 WB; tested species: rat
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 106 211 WB
Short-term plasticity at cerebellar granule cell to molecular layer interneuron synapses expands information processing.
Dorgans K, Demais V, Bailly Y, Poulain B, Isope P, Doussau F
eLife (2019) 8: . 106 211 IHC, EM; tested species: mouse
Short-term plasticity at cerebellar granule cell to molecular layer interneuron synapses expands information processing.
Dorgans K, Demais V, Bailly Y, Poulain B, Isope P, Doussau F
eLife (2019) 8: . 106 211 IHC, EM; tested species: mouse
WB
ICC
IHC
IHC-P
Background

Synapsins are neuron-specific phosphoproteins that are exclusively associated with small synaptic vesicles, with little or no expression in other tissues including neuroendocrine cells. In mammals, three distinct synapsin genes (synapsin1, 2 and 3) encode more than eight neuronal isoforms.
Synapsin1 is one of the most specific markers of synapses throughout the central and peripheral nervous system. In addition to synaptic nerve terminals, the protein is also present in certain sensory nerve endings. It is expressed in two splice variants (synapsin1a and synapsin1b). Synapsin1 interacts with vesicle membranes as well as with actin and spectrin.
Synapsin2 is expressed in the nervous system and also two splice variants were described so far, while synapsin3 shows a more restricted expression pattern and is mainly found in the hippocampus.
Synapsins are major phosphoproteins and are substrates for several protein kinases such as PKA, CaMK I and CaMK II. Synapsin1 is widely used as reference substrate for calmodulin-dependent protein kinases.
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