Cat. No. 106 104 |
100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 up to 1 : 1000 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 up to 1 : 1000 gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 500 gallery Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.', $event)" style="cursor: help;">EM: yes |
Immunogen | Synthetic peptide corresponding to AA 445 to 462 from mouse Synapsin1 (UniProt Id: O88935) |
Reactivity |
Reacts with: mouse (O88935), rat (P09951). Other species not tested yet. |
Specificity | Specific for synapsins 1a and 1b, no cross-reactivity to synapsin 2a/b. K.O. validated |
Matching control protein/peptide | 106-1P |
Data sheet | 106_104.pdf |
Synapsins are neuron-specific phosphoproteins that are exclusively associated with small synaptic vesicles, with little or no expression in other tissues including neuroendocrine cells. In mammals, three distinct synapsin genes (synapsin1, 2, and 3) encode more than eight neuronal isoforms.
Synapsin1 is one of the most specific markers of synapses throughout the central and peripheral nervous system. In addition to synaptic nerve terminals, the protein is also present in certain sensory nerve endings. It is expressed in two splice variants (synapsin 1a and synapsin 1b). Synapsin1 interacts with vesicle membranes as well as with actin and spectrin.
Synapsin2 is expressed in the nervous system and also two splice variants were described so far, while synapsin3 shows a more restricted expression pattern and is mainly found in the hypocampus.
Synapsins are major phosphoproteins and are substrates for several protein kinases such as PKA, CaMK I and CaMK II. Synapsin1 is widely used as reference substrate for calmodulin-dependent protein kinases.