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Home > Products > 106 011BT

Synapsin1 antibody - 106 011BT K.O.

Synapsins are peripheral synaptic vesicle proteins and substrate for several protein kinases
Mouse monoclonal purified IgG
Cat. No.: 106 011BT
Amount: 100 µg
Price: $475.00
Cat. No. 106 011BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

 Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 100 up to 1 : 2000 gallery  
IHC: 1 : 200 up to 1 : 10000 gallery  
IHC-P: 1 : 1000 gallery  
Label biotin
Clone 46.1
Subtype IgG1
Immunogen full-length recombinant rat Synapsin1 (UniProt Id: P09951)
Epitop AA 435 to 475 from rat Synapsin1 (UniProt Id: P09951)
Reactivity Reacts with: human (P17600), rat (P09951), mouse (O88935), mammals.
Weaker signal: zebrafish, chicken, other vertebrates.
Other species not tested yet.
Specificity Specific for synapsin 1a and 1b independent of phosphorylation state. K.O. validated
Remarks

IP: Avidin or streptavidin beads are recommended.
Data sheet 106_011bt.pdf

References for Synapsin1 - 106 011BT

Neuronal BIN1 Regulates Presynaptic Neurotransmitter Release and Memory Consolidation.
De Rossi P, Nomura T, Andrew RJ, Masse NY, Sampathkumar V, Musial TF, Sudwarts A, Recupero AJ, Le Metayer T, Hansen MT, Shim HN, et al.
Cell reports (2020) 3010: 3520-3535.e7. 106 011BT ICC, IHC; tested species: mouse
Neuronal BIN1 Regulates Presynaptic Neurotransmitter Release and Memory Consolidation.
De Rossi P, Nomura T, Andrew RJ, Masse NY, Sampathkumar V, Musial TF, Sudwarts A, Recupero AJ, Le Metayer T, Hansen MT, Shim HN, et al.
Cell reports (2020) 3010: 3520-3535.e7. 106 011BT ICC, IHC; tested species: mouse
Cat. No.: 106 011BT
Amount: 100 µg
Price: $475.00
Neuronal BIN1 Regulates Presynaptic Neurotransmitter Release and Memory Consolidation.
De Rossi P, Nomura T, Andrew RJ, Masse NY, Sampathkumar V, Musial TF, Sudwarts A, Recupero AJ, Le Metayer T, Hansen MT, Shim HN, et al.
Cell reports (2020) 3010: 3520-3535.e7. 106 011BT ICC, IHC; tested species: mouse
Neuronal BIN1 Regulates Presynaptic Neurotransmitter Release and Memory Consolidation.
De Rossi P, Nomura T, Andrew RJ, Masse NY, Sampathkumar V, Musial TF, Sudwarts A, Recupero AJ, Le Metayer T, Hansen MT, Shim HN, et al.
Cell reports (2020) 3010: 3520-3535.e7. 106 011BT ICC, IHC; tested species: mouse
WB
ICC
IHC
IHC-P
Background

Synapsins are neuron-specific phosphoproteins that are exclusively associated with small synaptic vesicles, with little or no expression in other tissues including neuroendocrine cells. In mammals, three distinct synapsin genes (synapsin1, 2, and 3) encode more than eight neuronal isoforms.
Synapsin1 is one of the most specific markers of synapses throughout the central and peripheral nervous system. In addition to synaptic nerve terminals, the protein is also present in certain sensory nerve endings. It is expressed in two splice variants (synapsin 1a and synapsin 1b). Synapsin1 interacts with vesicle membranes as well as with actin and spectrin.
Synapsin2 is expressed in the nervous system and also two splice variants were described so far, while synapsin3 shows a more restricted expression pattern and is mainly found in the hypocampus.
Synapsins are major phosphoproteins and are substrates for several protein kinases such as PKA, CaMK I and CaMK II. Synapsin1 is widely used as reference substrate for calmodulin-dependent protein kinases.
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