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Home > Products > 119 202

SV2 C antibody - 119 202

SV2s are highly glycosylated synaptic vesicle proteins with homology to transmembrane transporters
Rabbit polyclonal antiserum
Cat. No.: 119 202
Amount: 200 µl
Price: $360.00
Cat. No. 119 202 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 200 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 2 to 16 from rat SV2C (UniProt Id: Q9Z2I6)
Reactivity Reacts with: human (Q496J9), rat (Q9Z2I6), mouse (Q69ZS6), cow, dog.
Other species not tested yet.
Matching control protein/peptide 119-2P
Remarks

WB:  SV2 C aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 119_202.pdf

References for SV2 C - 119 202

A new method for isolation and purification of fusion-competent inhibitory synaptic vesicles.
Gopal N, Leitz J, Wang C, Esquivies L, Pfuetzner RA, Brunger AT
Current research in physiology (2024) 7: 100121. 119 202 WB; tested species: mouse
Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology.
Ng XY, Wu Y, Lin Y, Yaqoob SM, Greene LE, De Camilli P, Cao M
NPJ Parkinson's disease (2023) 91: 26. 119 202 WB, IHC; tested species: mouse
Tetanus toxin and botulinum toxin a utilize unique mechanisms to enter neurons of the central nervous system.
Blum FC, Chen C, Kroken AR, Barbieri JT
Infection and immunity (2012) 805: 1662-9. 119 202 WB
Quantitative comparison of glutamatergic and GABAergic synaptic vesicles unveils selectivity for few proteins including MAL2, a novel synaptic vesicle protein.
Grønborg M, Pavlos NJ, Brunk I, Chua JJ, Münster-Wandowski A, Riedel D, Ahnert-Hilger G, Urlaub H, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 301: 2-12. 119 202 WB, ICC, IHC
Botulinum neurotoxins C, E and F bind gangliosides via a conserved binding site prior to stimulation-dependent uptake with botulinum neurotoxin F utilising the three isoforms of SV2 as second receptor.
Rummel A, Häfner K, Mahrhold S, Darashchonak N, Holt M, Jahn R, Beermann S, Karnath T, Bigalke H, Binz T
Journal of neurochemistry (2009) 1106: 1942-54. 119 202 WB; tested species: mouse
Distinct synaptic vesicle recycling in inhibitory nerve terminals is coordinated by SV2A.
Bae JR, Lee W, Jo YO, Han S, Koh S, Song WK, Kim SH
Progress in neurobiology (2020) : 101879. 119 202 ICC; tested species: rat
Mechanism and effects of pulsatile GABA secretion from cytosolic pools in the human beta cell.
Menegaz D, Hagan DW, Almaça J, Cianciaruso C, Rodriguez-Diaz R, Molina J, Dolan RM, Becker MW, Schwalie PC, Nano R, Lebreton F, et al.
Nature metabolism (2019) 111: 1110-1126. 119 202 ICC; tested species: rat
Quantitative comparison of glutamatergic and GABAergic synaptic vesicles unveils selectivity for few proteins including MAL2, a novel synaptic vesicle protein.
Grønborg M, Pavlos NJ, Brunk I, Chua JJ, Münster-Wandowski A, Riedel D, Ahnert-Hilger G, Urlaub H, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 301: 2-12. 119 202 WB, ICC, IHC
Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology.
Ng XY, Wu Y, Lin Y, Yaqoob SM, Greene LE, De Camilli P, Cao M
NPJ Parkinson's disease (2023) 91: 26. 119 202 WB, IHC; tested species: mouse
Expression of SV2 isoforms during rodent brain development.
Crèvecœur J, Foerch P, Doupagne M, Thielen C, Vandenplas C, Moonen G, Deprez M, Rogister B
BMC neuroscience (2013) 14: 87. 119 202 IHC
Quantitative comparison of glutamatergic and GABAergic synaptic vesicles unveils selectivity for few proteins including MAL2, a novel synaptic vesicle protein.
Grønborg M, Pavlos NJ, Brunk I, Chua JJ, Münster-Wandowski A, Riedel D, Ahnert-Hilger G, Urlaub H, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 301: 2-12. 119 202 WB, ICC, IHC
Cat. No.: 119 202
Amount: 200 µl
Price: $360.00
A new method for isolation and purification of fusion-competent inhibitory synaptic vesicles.
Gopal N, Leitz J, Wang C, Esquivies L, Pfuetzner RA, Brunger AT
Current research in physiology (2024) 7: 100121. 119 202 WB; tested species: mouse
Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology.
Ng XY, Wu Y, Lin Y, Yaqoob SM, Greene LE, De Camilli P, Cao M
NPJ Parkinson's disease (2023) 91: 26. 119 202 WB, IHC; tested species: mouse
Tetanus toxin and botulinum toxin a utilize unique mechanisms to enter neurons of the central nervous system.
Blum FC, Chen C, Kroken AR, Barbieri JT
Infection and immunity (2012) 805: 1662-9. 119 202 WB
Quantitative comparison of glutamatergic and GABAergic synaptic vesicles unveils selectivity for few proteins including MAL2, a novel synaptic vesicle protein.
Grønborg M, Pavlos NJ, Brunk I, Chua JJ, Münster-Wandowski A, Riedel D, Ahnert-Hilger G, Urlaub H, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 301: 2-12. 119 202 WB, ICC, IHC
Botulinum neurotoxins C, E and F bind gangliosides via a conserved binding site prior to stimulation-dependent uptake with botulinum neurotoxin F utilising the three isoforms of SV2 as second receptor.
Rummel A, Häfner K, Mahrhold S, Darashchonak N, Holt M, Jahn R, Beermann S, Karnath T, Bigalke H, Binz T
Journal of neurochemistry (2009) 1106: 1942-54. 119 202 WB; tested species: mouse
Distinct synaptic vesicle recycling in inhibitory nerve terminals is coordinated by SV2A.
Bae JR, Lee W, Jo YO, Han S, Koh S, Song WK, Kim SH
Progress in neurobiology (2020) : 101879. 119 202 ICC; tested species: rat
Mechanism and effects of pulsatile GABA secretion from cytosolic pools in the human beta cell.
Menegaz D, Hagan DW, Almaça J, Cianciaruso C, Rodriguez-Diaz R, Molina J, Dolan RM, Becker MW, Schwalie PC, Nano R, Lebreton F, et al.
Nature metabolism (2019) 111: 1110-1126. 119 202 ICC; tested species: rat
Quantitative comparison of glutamatergic and GABAergic synaptic vesicles unveils selectivity for few proteins including MAL2, a novel synaptic vesicle protein.
Grønborg M, Pavlos NJ, Brunk I, Chua JJ, Münster-Wandowski A, Riedel D, Ahnert-Hilger G, Urlaub H, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 301: 2-12. 119 202 WB, ICC, IHC
Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology.
Ng XY, Wu Y, Lin Y, Yaqoob SM, Greene LE, De Camilli P, Cao M
NPJ Parkinson's disease (2023) 91: 26. 119 202 WB, IHC; tested species: mouse
Expression of SV2 isoforms during rodent brain development.
Crèvecœur J, Foerch P, Doupagne M, Thielen C, Vandenplas C, Moonen G, Deprez M, Rogister B
BMC neuroscience (2013) 14: 87. 119 202 IHC
Quantitative comparison of glutamatergic and GABAergic synaptic vesicles unveils selectivity for few proteins including MAL2, a novel synaptic vesicle protein.
Grønborg M, Pavlos NJ, Brunk I, Chua JJ, Münster-Wandowski A, Riedel D, Ahnert-Hilger G, Urlaub H, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 301: 2-12. 119 202 WB, ICC, IHC
WB
ICC
IHC
Background

SV2s (Synaptic Vesicle Protein 2) are integral membrane glycoproteins present in synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis (1). There are three characterized isoforms, SV2 A, SV2 B and SV2 C that are similar in structure but show different expression patterns. SV2 A is expressed ubiquitously throughout the brain and plays a crucial role in modulating synaptic transmission by regulating the expression and trafficking of synaptotagmin, a key calcium sensor in neurotransmitter release (1).
SV2 B has a more restricted distribution with varying degrees of coexpression with SV2 A and is predominantly found in the cortex and hippocampus (2). SV2 C is more closely related to SV2 A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb (3).
SV2 expression has also been observed in other non-neuronal organs. In kidney it localizes to podocytes and is essential for the integrity of the glomerular filtration barrier (4).
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