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Home > Products > 119 103

SV2 B antibody - 119 103 K.O.

SV2s are highly glycosylated synaptic vesicle proteins with homology to transmembrane transporters
Rabbit polyclonal purified antibody
Cat. No.: 119 103
Amount: 50 µg
Price: $455.00
Cat. No. 119 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 2 to 17 from rat SV2B (UniProt Id: Q63564)
Reactivity Reacts with: human (Q7L1I2), rat (Q63564), mouse (Q8BG39), hamster, chicken.
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 119-1P
Remarks

WB: SV2 B aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 119_103.pdf

References for SV2 B - 119 103

A new method for isolation and purification of fusion-competent inhibitory synaptic vesicles.
Gopal N, Leitz J, Wang C, Esquivies L, Pfuetzner RA, Brunger AT
Current research in physiology (2024) 7: 100121. 119 103 WB; tested species: mouse
Human-Relevant Sensitivity of iPSC-Derived Human Motor Neurons to BoNT/A1 and B1.
Schenke M, Prause HC, Bergforth W, Przykopanski A, Rummel A, Klawonn F, Seeger B
Toxins (2021) 138: . 119 103 WB; tested species: human
Reductions in Synaptic Vesicle Glycoprotein 2 Isoforms in the Cortex and Hippocampus in a Rat Model of Traumatic Brain Injury.
Fronczak KM, Li Y, Henchir J, Dixon CE, Carlson SW
Molecular neurobiology (2021) : . 119 103 WB, IHC; tested species: rat
CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.
Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C, Cousin MA, Gundelfinger ED, Rosenmund C, Cooper B, Fejtova A, et al.
Cell reports (2020) 307: 2444-2459.e7. 119 103 ICC; tested species: mouse
Reductions in Synaptic Vesicle Glycoprotein 2 Isoforms in the Cortex and Hippocampus in a Rat Model of Traumatic Brain Injury.
Fronczak KM, Li Y, Henchir J, Dixon CE, Carlson SW
Molecular neurobiology (2021) : . 119 103 WB, IHC; tested species: rat
Cat. No.: 119 103
Amount: 50 µg
Price: $455.00
A new method for isolation and purification of fusion-competent inhibitory synaptic vesicles.
Gopal N, Leitz J, Wang C, Esquivies L, Pfuetzner RA, Brunger AT
Current research in physiology (2024) 7: 100121. 119 103 WB; tested species: mouse
Human-Relevant Sensitivity of iPSC-Derived Human Motor Neurons to BoNT/A1 and B1.
Schenke M, Prause HC, Bergforth W, Przykopanski A, Rummel A, Klawonn F, Seeger B
Toxins (2021) 138: . 119 103 WB; tested species: human
Reductions in Synaptic Vesicle Glycoprotein 2 Isoforms in the Cortex and Hippocampus in a Rat Model of Traumatic Brain Injury.
Fronczak KM, Li Y, Henchir J, Dixon CE, Carlson SW
Molecular neurobiology (2021) : . 119 103 WB, IHC; tested species: rat
CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.
Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C, Cousin MA, Gundelfinger ED, Rosenmund C, Cooper B, Fejtova A, et al.
Cell reports (2020) 307: 2444-2459.e7. 119 103 ICC; tested species: mouse
Reductions in Synaptic Vesicle Glycoprotein 2 Isoforms in the Cortex and Hippocampus in a Rat Model of Traumatic Brain Injury.
Fronczak KM, Li Y, Henchir J, Dixon CE, Carlson SW
Molecular neurobiology (2021) : . 119 103 WB, IHC; tested species: rat
WB
ICC
IHC
IHC
IHC-P
Background

SV2s (synaptic vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2 A, SV2 B and SV2 C. SV2 A is expressed ubiquitously throughout the brain and is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium- stimulated exocytosis. SV2 B has a more restricted distribution with varying degrees of coexpression with SV2 A. SV2 C is more closely related to SV2 A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2 expression has also been observed in other organs. In kidney it localizes to podocytes.
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