Synaptic Systems

Cat. No.: 268 003

Amount: 50 µg

Price: $375.00

WB

Background

The Small ubiquitin-related modifiers SUMOs are ubiquitin-like proteins (UBLs) that can be conjugated to other proteins in a manner analogous to ubiquitin. Four SUMO paralogs, SUMO 1-4 have been described in mammals. SUMO 1 is also referred to as GMP 1, Setrin 1, SMT3C and SMT3H3.
Modification of proteins with SUMO 1 or other SUMOs requires a unique activating enzyme complex as well as a conjugation enzyme. This process plays an important role in a wide variety of processes such as transcriptional regulation, maintenance of genome integrity and subcellular localization.
Sumoylated proteins include PML, Sp100, IκBα, RanGAP1and RANBP2. The unmodified 70 kDa form of RanGAP1 is exclusively cytoplasmic, whereas the 90 kDa sumoylated form is associated with the cytoplasmic fibers of nuclear pore complexes (NPCs).

1 ) Yuan H et al., Cell Res. (2010)
2 ) Riising EM et al., PLoS ONE (2008)
3 ) Brown PW et al., Hum. Reprod. (2008)
4 ) Riley BE et al., J. Biol. Chem. (2005)
5 ) Saitoh H et al., J. Biol. Chem. (2002)

Protocols

WB

OTHER

Preabsorbtion

Other products for this target

Cat. No. 268 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not recommended IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 57 to 70 from mouse SUMO1 (UniProt Id: P63166)
Reactivity	Reacts with: human (P63165), rat (Q5I0H3), mouse (P63166). Other species not tested yet.
Matching control protein/peptide	268-0P
Data sheet	268_003.pdf

SUMO1 antibody - 268 003