Synaptic Systems

Cat. No.: 268 003

Amount: 50 µg

Price: $375.00

WB

Background

The Small ubiquitin-related modifiers SUMOs are ubiquitin-like proteins (UBLs) that can be conjugated to other proteins in a manner analogous to ubiquitin. Four SUMO paralogs, SUMO 1-4 have been described in mammals. SUMO 1 is also referred to as GMP 1, Setrin 1, SMT3C and SMT3H3.
Modification of proteins with SUMO 1 or other SUMOs requires a unique activating enzyme complex as well as a conjugation enzyme. This process plays an important role in a wide variety of processes such as transcriptional regulation, maintenance of genome integrity and subcellular localization.
Sumoylated proteins include PML, Sp100, IκBα, RanGAP1and RANBP2. The unmodified 70 kDa form of RanGAP1 is exclusively cytoplasmic, whereas the 90 kDa sumoylated form is associated with the cytoplasmic fibers of nuclear pore complexes (NPCs).

1 ) Yuan H, Cell research (2010) 202: 185-96.
2 ) Riising EM, PloS one (2008) 37: e2704.
3 ) Brown PW, Human reproduction (Oxford, England) (2008) 2312: 2850-7.
4 ) Riley BE, The Journal of biological chemistry (2005) 28023: 21942-8.
5 ) Saitoh H, The Journal of biological chemistry (2002) 2777: 4755-63.

Protocols

WB

OTHER

Preabsorbtion

Other products for this target

Cat. No. 268 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not recommended IHC: not recommended IHC-P: not tested yet
Immunogen	Synthetic peptide corresponding to AA 57 to 70 from mouse SUMO1 (UniProt Id: P63166)
Reactivity	Reacts with: human (P63165), rat (Q5I0H3), mouse (P63166). Other species not tested yet.
Matching control protein/peptide	268-0P
Data sheet	268_003.pdf

SUMO1 antibody - 268 003