Synaptic Systems

Cat. No.: 268-0P

Amount: 100 µg

Price: $105.00

Background

The Small ubiquitin-related modifiers SUMOs are ubiquitin-like proteins (UBLs) that can be conjugated to other proteins in a manner analogous to ubiquitin. Four SUMO paralogs, SUMO 1-4 have been described in mammals. SUMO 1 is also referred to as GMP 1, Setrin 1, SMT3C and SMT3H3.
Modification of proteins with SUMO 1 or other SUMOs requires a unique activating enzyme complex as well as a conjugation enzyme. This process plays an important role in a wide variety of processes such as transcriptional regulation, maintenance of genome integrity and subcellular localization.
Sumoylated proteins include PML, Sp100, IκBα, RanGAP1and RANBP2. The unmodified 70 kDa form of RanGAP1 is exclusively cytoplasmic, whereas the 90 kDa sumoylated form is associated with the cytoplasmic fibers of nuclear pore complexes (NPCs).

1 ) Yuan H et al., Cell Res. (2010)
2 ) Riising EM et al., PLoS ONE (2008)
3 ) Brown PW et al., Hum. Reprod. (2008)
4 ) Riley BE et al., J. Biol. Chem. (2005)
5 ) Saitoh H et al., J. Biol. Chem. (2002)

Protocols

OTHER

Preadsorbtion

Other products for this target

Cat. No. 268-0P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 57 to 70 from mouse SUMO1 (UniProt Id: P63166)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	268 003
Remarks	This control peptide consists of the synthetic peptide (aa 57-70 of mouse SUMO 1) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	268-0p.pdf

SUMO1 peptide - 268-0P