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Home > Products > 148 102

Snapin antibody - 148 102

Snapin is involved in SNARE mediated vesicle fusion
Rabbit polyclonal antiserum
Cat. No.: 148 102
Amount: 200 µl
Price: $355.00
Cat. No. 148 102 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 5000 (AP staining) gallery  
IP: not recommended
ICC: not tested yet
IHC: not recommended
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 117 to 136 from rat Snapin (UniProt Id: P60192)
Reactivity Reacts with: human (O95295), rat (P60192), mouse (Q9Z266).
Other species not tested yet.
Matching control protein/peptide 148-1P
Remarks

Since snapin is present in very low concentrations long exposure time is recommended.
Data sheet 148_102.pdf

References for Snapin - 148 102

Snapin recruits dynein to BDNF-TrkB signaling endosomes for retrograde axonal transport and is essential for dendrite growth of cortical neurons.
Zhou B, Cai Q, Xie Y, Sheng ZH
Cell reports (2012) 21: 42-51. 148 102 WB; tested species: mouse
The role for HNF-1beta-targeted collectrin in maintenance of primary cilia and cell polarity in collecting duct cells.
Zhang Y, Wada J, Yasuhara A, Iseda I, Eguchi J, Fukui K, Yang Q, Yamagata K, Hiesberger T, Igarashi P, Zhang H, et al.
PloS one (2007) 25: e414. 148 102 WB
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 148 102 WB
A novel role for snapin in dendrite patterning: interaction with cypin.
Chen M, Lucas KG, Akum BF, Balasingam G, Stawicki TM, Provost JM, Riefler GM, Jörnsten RJ, Firestein BL
Molecular biology of the cell (2005) 1611: 5103-14. 148 102 WB; tested species: rat
Reinvestigation of the role of snapin in neurotransmitter release.
Vites O, Rhee JS, Schwarz M, Rosenmund C, Jahn R
The Journal of biological chemistry (2004) 27925: 26251-6. 148 102 WB
Cat. No.: 148 102
Amount: 200 µl
Price: $355.00
Snapin recruits dynein to BDNF-TrkB signaling endosomes for retrograde axonal transport and is essential for dendrite growth of cortical neurons.
Zhou B, Cai Q, Xie Y, Sheng ZH
Cell reports (2012) 21: 42-51. 148 102 WB; tested species: mouse
The role for HNF-1beta-targeted collectrin in maintenance of primary cilia and cell polarity in collecting duct cells.
Zhang Y, Wada J, Yasuhara A, Iseda I, Eguchi J, Fukui K, Yang Q, Yamagata K, Hiesberger T, Igarashi P, Zhang H, et al.
PloS one (2007) 25: e414. 148 102 WB
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 148 102 WB
A novel role for snapin in dendrite patterning: interaction with cypin.
Chen M, Lucas KG, Akum BF, Balasingam G, Stawicki TM, Provost JM, Riefler GM, Jörnsten RJ, Firestein BL
Molecular biology of the cell (2005) 1611: 5103-14. 148 102 WB; tested species: rat
Reinvestigation of the role of snapin in neurotransmitter release.
Vites O, Rhee JS, Schwarz M, Rosenmund C, Jahn R
The Journal of biological chemistry (2004) 27925: 26251-6. 148 102 WB
WB
Background

Snapin, also referred to as Snapap, was initially identified as a SNAP 25 interacting protein which enhances the binding of synaptotagmin 1 to SNAREs in a phosphorylation dependent manner. Later an ubiquitous expression pattern in neuronal and non-neuronal cells and interaction with SNAP 23 was described. The protein contains heptad repeats typical for coiled coils in its C-terminal part.
The role of this protein in SNARE mediated fusion is still under discussion.
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