Cat. No.: 148 002
Amount: 200 µl
Price:
$355.00
Cat. No. 148 002 |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: yes Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 500 gallery |
Immunogen | Recombinant protein corresponding to AA 1 to 136 from rat Snapin (UniProt Id: P60192) |
Reactivity |
Reacts with: human (O95295), rat (P60192), mouse (Q9Z266), rabbit. No signal: zebrafish. Other species not tested yet. |
Specificity | K.O. validated PubMed: 20946101 |
Matching control protein/peptide | 148-0P |
Remarks |
Since snapin is present in very low concentrations long exposure time is recommended. |
Data sheet | 148_002.pdf |
Snapin, also referred to as Snapap, was initially identified as a SNAP 25 interacting protein which enhances the binding of synaptotagmin 1 to SNAREs in a phosphorylation dependent manner. Later an ubiquitous expression pattern in neuronal and non-neuronal cells and interaction with SNAP 23 was described. The protein contains heptad repeats typical for coiled coils in its C-terminal part.
The role of this protein in SNARE mediated fusion is still under discussion.