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Home > Products > 148 002

Snapin antibody - 148 002 K.O.

Snapin is involved in SNARE mediated vesicle fusion
Rabbit polyclonal antiserum
Cat. No.: 148 002
Amount: 200 µl
Price: $355.00
Cat. No. 148 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 5000
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 136 from rat Snapin (UniProt Id: P60192)
Reactivity Reacts with: human (O95295), rat (P60192), mouse (Q9Z266), rabbit.
No signal: zebrafish.
Other species not tested yet.
Specificity K.O. validated PubMed: 20946101
Matching control protein/peptide 148-0P
Remarks

Since snapin is present in very low concentrations long exposure time is recommended.
Data sheet 148_002.pdf

References for Snapin - 148 002

Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.
Matsui T, Sakamaki Y, Nakashima S, Fukuda M
Cell reports (2022) 399: 110875. 148 002 WB; tested species: human
A Ragulator-BORC interaction controls lysosome positioning in response to amino acid availability.
Pu J, Keren-Kaplan T, Bonifacino JS
The Journal of cell biology (2017) 21612: 4183-4197. 148 002 WB; tested species: human
Dysbindin-associated proteome in the p2 synaptosome fraction of mouse brain.
Han MH, Hu Z, Chen CY, Chen Y, Gucek M, Li Z, Markey SP
Journal of proteome research (2014) 1311: 4567-80. 148 002 WB; tested species: mouse
Snapin, positive regulator of stimulation- induced Ca²⁺ release through RyR, is necessary for HIV-1 replication in T cells.
Kinoshita SM, Kogure A, Taguchi S, Nolan GP
PloS one (2013) 810: e75297. 148 002 WB, IP, ICC; tested species: human
LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25.
Yun HJ, Park J, Ho DH, Kim H, Kim CH, Oh H, Ga I, Seo H, Chang S, Son I, Seol W, et al.
Experimental & molecular medicine (2013) 45: e36. 148 002 IP, WB; tested species: mouse
Snapin deficiency is associated with developmental defects of the central nervous system.
Zhou B, Zhu YB, Lin L, Cai Q, Sheng ZH
Bioscience reports (2011) 312: 151-8. 148 002 WB; KO verified; tested species: mouse
The SNARE-associated component SNAPIN binds PUMILIO2 and NANOS1 proteins in human male germ cells.
Ginter-Matuszewska B, Spik A, Rembiszewska A, Koyias C, Kupryjanczyk J, Jaruzelska J
Molecular human reproduction (2009) 153: 173-9. 148 002 WB, IHC-P; tested species: human,mouse
Snapin interacts with the Exo70 subunit of the exocyst and modulates GLUT4 trafficking.
Bao Y, Lopez JA, James DE, Hunziker W
The Journal of biological chemistry (2008) 2831: 324-31. 148 002 WB, ICC
The UT-A1 urea transporter interacts with snapin, a SNARE-associated protein.
Mistry AC, Mallick R, Fröhlich O, Klein JD, Rehm A, Chen G, Sands JM
The Journal of biological chemistry (2007) 28241: 30097-106. 148 002 WB, ICC; tested species: rabbit
The TRPM7 ion channel functions in cholinergic synaptic vesicles and affects transmitter release.
Krapivinsky G, Mochida S, Krapivinsky L, Cibulsky SM, Clapham DE
Neuron (2006) 523: 485-96. 148 002 WB
A novel role for snapin in dendrite patterning: interaction with cypin.
Chen M, Lucas KG, Akum BF, Balasingam G, Stawicki TM, Provost JM, Riefler GM, Jörnsten RJ, Firestein BL
Molecular biology of the cell (2005) 1611: 5103-14. 148 002 ICC, WB; tested species: rat
Targeting of the GTPase Irgm1 to the phagosomal membrane via PtdIns(3,4)P(2) and PtdIns(3,4,5)P(3) promotes immunity to mycobacteria.
Tiwari S, Choi HP, Matsuzawa T, Pypaert M, MacMicking JD
Nature immunology (2009) 108: 907-17. 148 002 Dot blot, ICC; tested species: mouse
Snapin, positive regulator of stimulation- induced Ca²⁺ release through RyR, is necessary for HIV-1 replication in T cells.
Kinoshita SM, Kogure A, Taguchi S, Nolan GP
PloS one (2013) 810: e75297. 148 002 WB, IP, ICC; tested species: human
LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25.
Yun HJ, Park J, Ho DH, Kim H, Kim CH, Oh H, Ga I, Seo H, Chang S, Son I, Seol W, et al.
Experimental & molecular medicine (2013) 45: e36. 148 002 IP, WB; tested species: mouse
Snapin, positive regulator of stimulation- induced Ca²⁺ release through RyR, is necessary for HIV-1 replication in T cells.
Kinoshita SM, Kogure A, Taguchi S, Nolan GP
PloS one (2013) 810: e75297. 148 002 WB, IP, ICC; tested species: human
Targeting of the GTPase Irgm1 to the phagosomal membrane via PtdIns(3,4)P(2) and PtdIns(3,4,5)P(3) promotes immunity to mycobacteria.
Tiwari S, Choi HP, Matsuzawa T, Pypaert M, MacMicking JD
Nature immunology (2009) 108: 907-17. 148 002 Dot blot, ICC; tested species: mouse
Snapin interacts with the Exo70 subunit of the exocyst and modulates GLUT4 trafficking.
Bao Y, Lopez JA, James DE, Hunziker W
The Journal of biological chemistry (2008) 2831: 324-31. 148 002 WB, ICC
The UT-A1 urea transporter interacts with snapin, a SNARE-associated protein.
Mistry AC, Mallick R, Fröhlich O, Klein JD, Rehm A, Chen G, Sands JM
The Journal of biological chemistry (2007) 28241: 30097-106. 148 002 WB, ICC; tested species: rabbit
A novel role for snapin in dendrite patterning: interaction with cypin.
Chen M, Lucas KG, Akum BF, Balasingam G, Stawicki TM, Provost JM, Riefler GM, Jörnsten RJ, Firestein BL
Molecular biology of the cell (2005) 1611: 5103-14. 148 002 ICC, WB; tested species: rat
Mice deficient in transmembrane prostatic acid phosphatase display increased GABAergic transmission and neurological alterations.
Nousiainen HO, Quintero IB, Myöhänen TT, Voikar V, Mijatovic J, Segerstråle M, Herrala AM, Kulesskaya N, Pulkka AE, Kivinummi T, Abo-Ramadan U, et al.
PloS one (2014) 95: e97851. 148 002 IHC; tested species: mouse
The SNARE-associated component SNAPIN binds PUMILIO2 and NANOS1 proteins in human male germ cells.
Ginter-Matuszewska B, Spik A, Rembiszewska A, Koyias C, Kupryjanczyk J, Jaruzelska J
Molecular human reproduction (2009) 153: 173-9. 148 002 WB, IHC-P; tested species: human,mouse
Cat. No.: 148 002
Amount: 200 µl
Price: $355.00
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.
Matsui T, Sakamaki Y, Nakashima S, Fukuda M
Cell reports (2022) 399: 110875. 148 002 WB; tested species: human
A Ragulator-BORC interaction controls lysosome positioning in response to amino acid availability.
Pu J, Keren-Kaplan T, Bonifacino JS
The Journal of cell biology (2017) 21612: 4183-4197. 148 002 WB; tested species: human
Dysbindin-associated proteome in the p2 synaptosome fraction of mouse brain.
Han MH, Hu Z, Chen CY, Chen Y, Gucek M, Li Z, Markey SP
Journal of proteome research (2014) 1311: 4567-80. 148 002 WB; tested species: mouse
Snapin, positive regulator of stimulation- induced Ca²⁺ release through RyR, is necessary for HIV-1 replication in T cells.
Kinoshita SM, Kogure A, Taguchi S, Nolan GP
PloS one (2013) 810: e75297. 148 002 WB, IP, ICC; tested species: human
LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25.
Yun HJ, Park J, Ho DH, Kim H, Kim CH, Oh H, Ga I, Seo H, Chang S, Son I, Seol W, et al.
Experimental & molecular medicine (2013) 45: e36. 148 002 IP, WB; tested species: mouse
Snapin deficiency is associated with developmental defects of the central nervous system.
Zhou B, Zhu YB, Lin L, Cai Q, Sheng ZH
Bioscience reports (2011) 312: 151-8. 148 002 WB; KO verified; tested species: mouse
The SNARE-associated component SNAPIN binds PUMILIO2 and NANOS1 proteins in human male germ cells.
Ginter-Matuszewska B, Spik A, Rembiszewska A, Koyias C, Kupryjanczyk J, Jaruzelska J
Molecular human reproduction (2009) 153: 173-9. 148 002 WB, IHC-P; tested species: human,mouse
Snapin interacts with the Exo70 subunit of the exocyst and modulates GLUT4 trafficking.
Bao Y, Lopez JA, James DE, Hunziker W
The Journal of biological chemistry (2008) 2831: 324-31. 148 002 WB, ICC
The UT-A1 urea transporter interacts with snapin, a SNARE-associated protein.
Mistry AC, Mallick R, Fröhlich O, Klein JD, Rehm A, Chen G, Sands JM
The Journal of biological chemistry (2007) 28241: 30097-106. 148 002 WB, ICC; tested species: rabbit
The TRPM7 ion channel functions in cholinergic synaptic vesicles and affects transmitter release.
Krapivinsky G, Mochida S, Krapivinsky L, Cibulsky SM, Clapham DE
Neuron (2006) 523: 485-96. 148 002 WB
A novel role for snapin in dendrite patterning: interaction with cypin.
Chen M, Lucas KG, Akum BF, Balasingam G, Stawicki TM, Provost JM, Riefler GM, Jörnsten RJ, Firestein BL
Molecular biology of the cell (2005) 1611: 5103-14. 148 002 ICC, WB; tested species: rat
Targeting of the GTPase Irgm1 to the phagosomal membrane via PtdIns(3,4)P(2) and PtdIns(3,4,5)P(3) promotes immunity to mycobacteria.
Tiwari S, Choi HP, Matsuzawa T, Pypaert M, MacMicking JD
Nature immunology (2009) 108: 907-17. 148 002 Dot blot, ICC; tested species: mouse
Snapin, positive regulator of stimulation- induced Ca²⁺ release through RyR, is necessary for HIV-1 replication in T cells.
Kinoshita SM, Kogure A, Taguchi S, Nolan GP
PloS one (2013) 810: e75297. 148 002 WB, IP, ICC; tested species: human
LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25.
Yun HJ, Park J, Ho DH, Kim H, Kim CH, Oh H, Ga I, Seo H, Chang S, Son I, Seol W, et al.
Experimental & molecular medicine (2013) 45: e36. 148 002 IP, WB; tested species: mouse
Snapin, positive regulator of stimulation- induced Ca²⁺ release through RyR, is necessary for HIV-1 replication in T cells.
Kinoshita SM, Kogure A, Taguchi S, Nolan GP
PloS one (2013) 810: e75297. 148 002 WB, IP, ICC; tested species: human
Targeting of the GTPase Irgm1 to the phagosomal membrane via PtdIns(3,4)P(2) and PtdIns(3,4,5)P(3) promotes immunity to mycobacteria.
Tiwari S, Choi HP, Matsuzawa T, Pypaert M, MacMicking JD
Nature immunology (2009) 108: 907-17. 148 002 Dot blot, ICC; tested species: mouse
Snapin interacts with the Exo70 subunit of the exocyst and modulates GLUT4 trafficking.
Bao Y, Lopez JA, James DE, Hunziker W
The Journal of biological chemistry (2008) 2831: 324-31. 148 002 WB, ICC
The UT-A1 urea transporter interacts with snapin, a SNARE-associated protein.
Mistry AC, Mallick R, Fröhlich O, Klein JD, Rehm A, Chen G, Sands JM
The Journal of biological chemistry (2007) 28241: 30097-106. 148 002 WB, ICC; tested species: rabbit
A novel role for snapin in dendrite patterning: interaction with cypin.
Chen M, Lucas KG, Akum BF, Balasingam G, Stawicki TM, Provost JM, Riefler GM, Jörnsten RJ, Firestein BL
Molecular biology of the cell (2005) 1611: 5103-14. 148 002 ICC, WB; tested species: rat
Mice deficient in transmembrane prostatic acid phosphatase display increased GABAergic transmission and neurological alterations.
Nousiainen HO, Quintero IB, Myöhänen TT, Voikar V, Mijatovic J, Segerstråle M, Herrala AM, Kulesskaya N, Pulkka AE, Kivinummi T, Abo-Ramadan U, et al.
PloS one (2014) 95: e97851. 148 002 IHC; tested species: mouse
The SNARE-associated component SNAPIN binds PUMILIO2 and NANOS1 proteins in human male germ cells.
Ginter-Matuszewska B, Spik A, Rembiszewska A, Koyias C, Kupryjanczyk J, Jaruzelska J
Molecular human reproduction (2009) 153: 173-9. 148 002 WB, IHC-P; tested species: human,mouse
ICC
IHC
IHC-P
Background

Snapin, also referred to as Snapap, was initially identified as a SNAP 25 interacting protein which enhances the binding of synaptotagmin 1 to SNAREs in a phosphorylation dependent manner. Later an ubiquitous expression pattern in neuronal and non-neuronal cells and interaction with SNAP 23 was described. The protein contains heptad repeats typical for coiled coils in its C-terminal part.
The role of this protein in SNARE mediated fusion is still under discussion.
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