SNAP25B antibody - 111 113 K.O.

SNAP25 is a palmitoylated plasma membrane protein; required for exocytosis

Rabbit polyclonal purified antibody

Cat. No.: 111 113

Amount: 50 µg

Price: $375.00

Cat. No. 111 113	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 58 to 72 from rat SNAP25B (UniProt Id: P60881-1)
Reactivity	Reacts with: rat (P60881-1), mouse (P60879-1). Other species not tested yet.
Specificity	Specific for SNAP 25B, no cross-reactivity to SNAP 25A. K.O. validated PubMed: 31024034
Data sheet	111_113.pdf

References for SNAP25B - 111 113

SNAP-25 isoforms differentially regulate synaptic transmission and long-term synaptic plasticity at central synapses.
Irfan M, Gopaul KR, Miry O, Hökfelt T, Stanton PK, Bark C
Scientific reports (2019) 91: 6403. 111 113 WB; KO verified; tested species: mouse

Cat. No.: 111 113

Amount: 50 µg

Price: $375.00

ICC

Background

SNAP25 (synaptosome-associated protein of 25 kDa) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 is the target of Botulinum neurotoxin A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP25A and 25B.

1 ) Lin RC et al., Annu. Rev. Cell Dev. Biol. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Jahn R et al., Annu. Rev. Biochem. (1999)
4 ) Fasshauer D et al., J. Biol. Chem. (1997)
5 ) Südhof TC et al., Nature (1995)
6 ) Blasi J et al., Nature (1993)
7 ) Prescott GR et al., BMC Neurosci (2011)
8 ) Schulze KL et al., Cell (1995)

Protocols

ICC