Synaptic Systems

Cat. No.: 111 213

Amount: 50 µg

Price: $375.00

Cat. No. 111 213	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes (see remarks) ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 86 to 210 from mouse SNAP23 (UniProt Id: O09044)
Reactivity	Reacts with: rat (O70377), mouse (O09044). Other species not tested yet.
Remarks	IP: This antibody is most efficient and recommended for immunoprecipitation.
Data sheet	111_213.pdf

References for SNAP23 - 111 213

EHD2 regulates plasma membrane integrity and downstream insulin receptor signalling events.
Neuhaus M, Fryklund C, Taylor H, Borreguero-Muñoz A, Kopietz F, Ardalani H, Rogova O, Stirrat L, Bremner SK, Spégel P, Bryant NJ, et al.
Molecular biology of the cell (2023) : mbcE23030078. 111 213 WB, ICC; tested species: mouse

SNAP23 regulates KCC2 membrane insertion and activity following mZnR/GPR39 activation in hippocampalneurons.
Asraf H, Bogdanovic M, Gottesman N, Sekler I, Aizenman E, Hershfinkel M
iScience (2022) 252: 103751. 111 213 IP, DNA-PAINT; tested species: mouse

Cat. No.: 111 213

Amount: 50 µg

Price: $375.00

Background

SNAP23 (synaptosome-associated protein of 23 kDa) is an ubiquitously expressed isoform and functional homologue of SNAP25. It is resistant to cleavage by BoNT/A and E. The protein is part of the exocytotic fusion complex (v-SNARE) where it assembles with syntaxin1 and synaptobrevin.
SNAP23 is able to function in regulated exocytosis. Both isoforms may have their own specific binding partners and discrete, albeit mechanistically similar, functional roles within the cell.

1 ) Lin RC et al., Annu. Rev. Cell Dev. Biol. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Jahn R et al., Annu. Rev. Biochem. (1999)
4 ) Sadoul K et al., J. Biol. Chem. (1997)
5 ) Chen F et al., Biochemistry (1997)
6 ) Ravichandran V et al., J. Biol. Chem. (1996)
7 ) Hanson PI et al., J. Biol. Chem. (1995)
8 ) Südhof TC et al., Nature (1995)
9 ) Rothman JE et al., Nature (1994)

Protocols

Standard protocol

Other products for this target

SNAP23 antibody - 111 213

References for SNAP23 - 111 213