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Shank3 antibody - 162 311

Shanks are multidomain proteins of the postsynaptic density
Mouse monoclonal purified IgG
Cat. No.: 162 311
Amount: 100 µg
Price: $415.00
Cat. No. 162 311 100 µg purified IgG, lyophilized. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not recommended
IHC-P: not tested yet
IHC-Fr: 1 : 500 (see remarks) gallery  
Clone 144B12
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat Shank3 (UniProt Id: Q9JLU4)
Reactivity Reacts with: rat (Q9JLU4).
Other species not tested yet.
Specificity Specific for Shank3
Remarks

IHC-Fr: Fixation with acetone or PFA/formaldehyde is recommended. Postfixation with methanol is not advised.

Data sheet 162_311.pdf

References for Shank3 - 162 311

SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 311 WB, IHC; tested species: mouse
A synaptic molecular dependency network in knockdown of autism- and schizophrenia-associated genes revealed by multiplexed imaging.
Falkovich R, Danielson EW, Perez de Arce K, Wamhoff EC, Strother J, Lapteva AP, Sheng M, Cottrell JR, Bathe M
Cell reports (2023) 425: 112430. 162 311 ICC; KD verified; tested species: rat
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 311 WB, IHC; tested species: mouse
Cat. No.: 162 311
Amount: 100 µg
Price: $415.00
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 311 WB, IHC; tested species: mouse
A synaptic molecular dependency network in knockdown of autism- and schizophrenia-associated genes revealed by multiplexed imaging.
Falkovich R, Danielson EW, Perez de Arce K, Wamhoff EC, Strother J, Lapteva AP, Sheng M, Cottrell JR, Bathe M
Cell reports (2023) 425: 112430. 162 311 ICC; KD verified; tested species: rat
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 311 WB, IHC; tested species: mouse