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Shank3 antibody - 162 304 K.O.

Shanks are multidomain proteins of the postsynaptic density
Guinea pig polyclonal antiserum
Cat. No.: 162 304
Amount: 100 µl
Price: $370.00
Cat. No. 162 304 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 2000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
IHC-Fr: 1 : 500 (see remarks) gallery  
ExM: 1 : 200 (see remarks) gallery  
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat Shank3 (UniProt Id: Q9JLU4)
Reactivity Reacts with: rat (Q9JLU4), mouse (Q4ACU6).
Other species not tested yet.
Specificity Antigen used for immunization is present in all Shank3 isoforms described for rat and in all isoforms described for mouse except Shank3-B and Shank3-C4. K.O. validated PubMed: 33115499
Remarks

IHC-Fr: Fixation with formaldehyde/PFA is recommended
ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)
Data sheet 162_304.pdf

References for Shank3 - 162 304

SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 304 WB, ICC, IHC; tested species: mouse
A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling.
Wu CH, Tatavarty V, Jean Beltran PM, Guerrero AA, Keshishian H, Krug K, MacMullan MA, Li L, Carr SA, Cottrell JR, Turrigiano GG, et al.
eLife (2022) 11: . 162 304 WB, IP, ICC; tested species: rat
Germline AGO2 mutations impair RNA interference and human neurological development.
Lessel D, Zeitler DM, Reijnders MRF, Kazantsev A, Hassani Nia F, Bartholomäus A, Martens V, Bruckmann A, Graus V, McConkie-Rosell A, McDonald M, et al.
Nature communications (2020) 111: 5797. 162 304 WB, ICC; KD verified; tested species: rat
Targeting of δ-catenin to postsynaptic sites through interaction with the Shank3 N-terminus.
Hassani Nia F, Woike D, Martens V, Klüssendorf M, Hönck HH, Harder S, Kreienkamp HJ
Molecular autism (2020) 111: 85. 162 304 WB; KO verified; tested species: mouse
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 162 304 WB, IHC; tested species: mouse
A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling.
Wu CH, Tatavarty V, Jean Beltran PM, Guerrero AA, Keshishian H, Krug K, MacMullan MA, Li L, Carr SA, Cottrell JR, Turrigiano GG, et al.
eLife (2022) 11: . 162 304 WB, IP, ICC; tested species: rat
Variants in LRRC7 lead to intellectual disability, autism, aggression and abnormal eating behaviors.
Willim J, Woike D, Greene D, Das S, Pfeifer K, Yuan W, Lindsey A, Itani O, Böhme AL, Tibbe D, Hönck HH, et al.
Nature communications (2024) 151: 7909. 162 304 ICC; tested species: rat
Polar lipids modify Alzheimer's Disease pathology by reducing astrocyte pro-inflammatory signaling through platelet-activating factor receptor (PTAFR) modulation.
Hans S, Stanton JE, Sauer AK, Shiels K, Saha SK, Lordan R, Tsoupras A, Zabetakis I, Grabrucker AM
Lipids in health and disease (2024) 231: 113. 162 304 ICC; tested species: rat
Jacob-induced transcriptional inactivation of CREB promotes Aβ-induced synapse loss in Alzheimer's disease.
Grochowska KM, Gomes GM, Raman R, Kaushik R, Sosulina L, Kaneko H, Oelschlegel AM, Yuanxiang P, Reyes-Resina I, Bayraktar G, Samer S, et al.
The EMBO journal (2023) : e112453. 162 304 ICC, IHC; tested species: mouse
Golgi satellites are essential for polysialylation of NCAM and expression of LTP at distal synapses.
Andres-Alonso M, Borgmeyer M, Mirzapourdelavar H, Lormann J, Klein K, Schweizer M, Hoffmeister-Ullerich S, Oelschlegel AM, Dityatev A, Kreutz MR
Cell reports (2023) 427: 112692. 162 304 ICC, IHC; tested species: mouse
Chaperone-mediated autophagy in neuronal dendrites utilizes activity-dependent lysosomal exocytosis for protein disposal.
Grochowska KM, Sperveslage M, Raman R, Failla AV, Głów D, Schulze C, Laprell L, Fehse B, Kreutz MR
Cell reports (2023) 428: 112998. 162 304 ICC; tested species: rat
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 304 WB, ICC, IHC; tested species: mouse
A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling.
Wu CH, Tatavarty V, Jean Beltran PM, Guerrero AA, Keshishian H, Krug K, MacMullan MA, Li L, Carr SA, Cottrell JR, Turrigiano GG, et al.
eLife (2022) 11: . 162 304 WB, IP, ICC; tested species: rat
Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment.
Borgmeyer M, Coman C, Has C, Schött HF, Li T, Westhoff P, Cheung YFH, Hoffmann N, Yuanxiang P, Behnisch T, Gomes GM, et al.
Cell reports (2021) 371: 109797. 162 304 ICC, IHC; tested species: mouse,rat
The histone demethylase PHF8 regulates astrocyte differentiation and function.
Iacobucci S, Padilla N, Gabrielli M, Navarro C, Lombardi M, Vicioso-Mantis M, Verderio C, de la Cruz X, Martínez-Balbás MA
Development (Cambridge, England) (2021) 14812: . 162 304 ICC; tested species: mouse
Ciliary neuropeptidergic signaling dynamically regulates excitatory synapses in postnatal neocortical pyramidal neurons.
Tereshko L, Gao Y, Cary BA, Turrigiano GG, Sengupta P
eLife (2021) 10: . 162 304 ICC; tested species: rat
Autism-associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses.
Bucher M, Niebling S, Han Y, Molodenskiy D, Hassani Nia F, Kreienkamp HJ, Svergun D, Kim E, Kostyukova AS, Kreutz MR, Mikhaylova M, et al.
eLife (2021) 10: . 162 304 ICC; tested species: rat
Germline AGO2 mutations impair RNA interference and human neurological development.
Lessel D, Zeitler DM, Reijnders MRF, Kazantsev A, Hassani Nia F, Bartholomäus A, Martens V, Bruckmann A, Graus V, McConkie-Rosell A, McDonald M, et al.
Nature communications (2020) 111: 5797. 162 304 WB, ICC; KD verified; tested species: rat
USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-dependent Protein Levels.
Kerrisk Campbell M, Sheng M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 162 304 ICC; tested species: rat
A septal-ventral tegmental area circuit drives exploratory behavior.
Mocellin P, Barnstedt O, Luxem K, Kaneko H, Vieweg S, Henschke JU, Dalügge D, Fuhrmann F, Karpova A, Pakan JMP, Kreutz MR, et al.
Neuron (2024) : . 162 304 IHC; tested species: mouse
Multisensory gamma stimulation mitigates the effects of demyelination induced by cuprizone in male mice.
Rodrigues-Amorim D, Bozzelli PL, Kim T, Liu L, Gibson O, Yang CY, Murdock MH, Galiana-Melendez F, Schatz B, Davison A, Islam MR, et al.
Nature communications (2024) 151: 6744. 162 304 IHC; tested species: mouse
Jacob-induced transcriptional inactivation of CREB promotes Aβ-induced synapse loss in Alzheimer's disease.
Grochowska KM, Gomes GM, Raman R, Kaushik R, Sosulina L, Kaneko H, Oelschlegel AM, Yuanxiang P, Reyes-Resina I, Bayraktar G, Samer S, et al.
The EMBO journal (2023) : e112453. 162 304 ICC, IHC; tested species: mouse
Golgi satellites are essential for polysialylation of NCAM and expression of LTP at distal synapses.
Andres-Alonso M, Borgmeyer M, Mirzapourdelavar H, Lormann J, Klein K, Schweizer M, Hoffmeister-Ullerich S, Oelschlegel AM, Dityatev A, Kreutz MR
Cell reports (2023) 427: 112692. 162 304 ICC, IHC; tested species: mouse
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 304 WB, ICC, IHC; tested species: mouse
Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment.
Borgmeyer M, Coman C, Has C, Schött HF, Li T, Westhoff P, Cheung YFH, Hoffmann N, Yuanxiang P, Behnisch T, Gomes GM, et al.
Cell reports (2021) 371: 109797. 162 304 ICC, IHC; tested species: mouse,rat
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 162 304 IHC; tested species: rat
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 162 304 WB, IHC; tested species: mouse
Cat. No.: 162 304
Amount: 100 µl
Price: $370.00
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 304 WB, ICC, IHC; tested species: mouse
A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling.
Wu CH, Tatavarty V, Jean Beltran PM, Guerrero AA, Keshishian H, Krug K, MacMullan MA, Li L, Carr SA, Cottrell JR, Turrigiano GG, et al.
eLife (2022) 11: . 162 304 WB, IP, ICC; tested species: rat
Germline AGO2 mutations impair RNA interference and human neurological development.
Lessel D, Zeitler DM, Reijnders MRF, Kazantsev A, Hassani Nia F, Bartholomäus A, Martens V, Bruckmann A, Graus V, McConkie-Rosell A, McDonald M, et al.
Nature communications (2020) 111: 5797. 162 304 WB, ICC; KD verified; tested species: rat
Targeting of δ-catenin to postsynaptic sites through interaction with the Shank3 N-terminus.
Hassani Nia F, Woike D, Martens V, Klüssendorf M, Hönck HH, Harder S, Kreienkamp HJ
Molecular autism (2020) 111: 85. 162 304 WB; KO verified; tested species: mouse
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 162 304 WB, IHC; tested species: mouse
A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling.
Wu CH, Tatavarty V, Jean Beltran PM, Guerrero AA, Keshishian H, Krug K, MacMullan MA, Li L, Carr SA, Cottrell JR, Turrigiano GG, et al.
eLife (2022) 11: . 162 304 WB, IP, ICC; tested species: rat
Variants in LRRC7 lead to intellectual disability, autism, aggression and abnormal eating behaviors.
Willim J, Woike D, Greene D, Das S, Pfeifer K, Yuan W, Lindsey A, Itani O, Böhme AL, Tibbe D, Hönck HH, et al.
Nature communications (2024) 151: 7909. 162 304 ICC; tested species: rat
Polar lipids modify Alzheimer's Disease pathology by reducing astrocyte pro-inflammatory signaling through platelet-activating factor receptor (PTAFR) modulation.
Hans S, Stanton JE, Sauer AK, Shiels K, Saha SK, Lordan R, Tsoupras A, Zabetakis I, Grabrucker AM
Lipids in health and disease (2024) 231: 113. 162 304 ICC; tested species: rat
Jacob-induced transcriptional inactivation of CREB promotes Aβ-induced synapse loss in Alzheimer's disease.
Grochowska KM, Gomes GM, Raman R, Kaushik R, Sosulina L, Kaneko H, Oelschlegel AM, Yuanxiang P, Reyes-Resina I, Bayraktar G, Samer S, et al.
The EMBO journal (2023) : e112453. 162 304 ICC, IHC; tested species: mouse
Golgi satellites are essential for polysialylation of NCAM and expression of LTP at distal synapses.
Andres-Alonso M, Borgmeyer M, Mirzapourdelavar H, Lormann J, Klein K, Schweizer M, Hoffmeister-Ullerich S, Oelschlegel AM, Dityatev A, Kreutz MR
Cell reports (2023) 427: 112692. 162 304 ICC, IHC; tested species: mouse
Chaperone-mediated autophagy in neuronal dendrites utilizes activity-dependent lysosomal exocytosis for protein disposal.
Grochowska KM, Sperveslage M, Raman R, Failla AV, Głów D, Schulze C, Laprell L, Fehse B, Kreutz MR
Cell reports (2023) 428: 112998. 162 304 ICC; tested species: rat
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 304 WB, ICC, IHC; tested species: mouse
A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling.
Wu CH, Tatavarty V, Jean Beltran PM, Guerrero AA, Keshishian H, Krug K, MacMullan MA, Li L, Carr SA, Cottrell JR, Turrigiano GG, et al.
eLife (2022) 11: . 162 304 WB, IP, ICC; tested species: rat
Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment.
Borgmeyer M, Coman C, Has C, Schött HF, Li T, Westhoff P, Cheung YFH, Hoffmann N, Yuanxiang P, Behnisch T, Gomes GM, et al.
Cell reports (2021) 371: 109797. 162 304 ICC, IHC; tested species: mouse,rat
The histone demethylase PHF8 regulates astrocyte differentiation and function.
Iacobucci S, Padilla N, Gabrielli M, Navarro C, Lombardi M, Vicioso-Mantis M, Verderio C, de la Cruz X, Martínez-Balbás MA
Development (Cambridge, England) (2021) 14812: . 162 304 ICC; tested species: mouse
Ciliary neuropeptidergic signaling dynamically regulates excitatory synapses in postnatal neocortical pyramidal neurons.
Tereshko L, Gao Y, Cary BA, Turrigiano GG, Sengupta P
eLife (2021) 10: . 162 304 ICC; tested species: rat
Autism-associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses.
Bucher M, Niebling S, Han Y, Molodenskiy D, Hassani Nia F, Kreienkamp HJ, Svergun D, Kim E, Kostyukova AS, Kreutz MR, Mikhaylova M, et al.
eLife (2021) 10: . 162 304 ICC; tested species: rat
Germline AGO2 mutations impair RNA interference and human neurological development.
Lessel D, Zeitler DM, Reijnders MRF, Kazantsev A, Hassani Nia F, Bartholomäus A, Martens V, Bruckmann A, Graus V, McConkie-Rosell A, McDonald M, et al.
Nature communications (2020) 111: 5797. 162 304 WB, ICC; KD verified; tested species: rat
USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-dependent Protein Levels.
Kerrisk Campbell M, Sheng M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 162 304 ICC; tested species: rat
A septal-ventral tegmental area circuit drives exploratory behavior.
Mocellin P, Barnstedt O, Luxem K, Kaneko H, Vieweg S, Henschke JU, Dalügge D, Fuhrmann F, Karpova A, Pakan JMP, Kreutz MR, et al.
Neuron (2024) : . 162 304 IHC; tested species: mouse
Multisensory gamma stimulation mitigates the effects of demyelination induced by cuprizone in male mice.
Rodrigues-Amorim D, Bozzelli PL, Kim T, Liu L, Gibson O, Yang CY, Murdock MH, Galiana-Melendez F, Schatz B, Davison A, Islam MR, et al.
Nature communications (2024) 151: 6744. 162 304 IHC; tested species: mouse
Jacob-induced transcriptional inactivation of CREB promotes Aβ-induced synapse loss in Alzheimer's disease.
Grochowska KM, Gomes GM, Raman R, Kaushik R, Sosulina L, Kaneko H, Oelschlegel AM, Yuanxiang P, Reyes-Resina I, Bayraktar G, Samer S, et al.
The EMBO journal (2023) : e112453. 162 304 ICC, IHC; tested species: mouse
Golgi satellites are essential for polysialylation of NCAM and expression of LTP at distal synapses.
Andres-Alonso M, Borgmeyer M, Mirzapourdelavar H, Lormann J, Klein K, Schweizer M, Hoffmeister-Ullerich S, Oelschlegel AM, Dityatev A, Kreutz MR
Cell reports (2023) 427: 112692. 162 304 ICC, IHC; tested species: mouse
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 304 WB, ICC, IHC; tested species: mouse
Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment.
Borgmeyer M, Coman C, Has C, Schött HF, Li T, Westhoff P, Cheung YFH, Hoffmann N, Yuanxiang P, Behnisch T, Gomes GM, et al.
Cell reports (2021) 371: 109797. 162 304 ICC, IHC; tested species: mouse,rat
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 162 304 IHC; tested species: rat
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 162 304 WB, IHC; tested species: mouse
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