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Home > Products > 162 302

Shank3 antibody - 162 302 K.O.

Shanks are multidomain proteins of the postsynaptic density
Rabbit polyclonal antiserum
Cat. No.: 162 302
Amount: 200 µl
Price: $355.00
Cat. No. 162 302 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
IHC-Fr: 1 : 500 (see remarks) gallery  
EM: yes
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat Shank3 (UniProt Id: Q9JLU4)
Reactivity Reacts with: rat (Q9JLU4), mouse (Q4ACU6).
Other species not tested yet.
Specificity K.O. validated
Remarks

IHC: This antibody requires mild fixation.
IHC-Fr: Fixation with PFA/formaldehyde is recommended
Data sheet 162_302.pdf

References for Shank3 - 162 302

Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome.
Yoon S, Santos MD, Forrest MP, Pratt CP, Khalatyan N, Mohler PJ, Savas JN, Penzes P
Cell reports (2023) 427: 112784. 162 302 WB, IP; tested species: mouse
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 302 WB, ICC, IHC; tested species: mouse
SREBP2 gene therapy targeting striatal astrocytes ameliorates Huntington's disease phenotypes.
Birolini G, Verlengia G, Talpo F, Maniezzi C, Zentilin L, Giacca M, Conforti P, Cordiglieri C, Caccia C, Leoni V, Taroni F, et al.
Brain : a journal of neurology (2021) 14410: 3175-3190. 162 302 WB; tested species: mouse
Zinc Stabilizes Shank3 at the Postsynaptic Density of Hippocampal Synapses.
Tao-Cheng JH, Toy D, Winters CA, Reese TS, Dosemeci A
PloS one (2016) 115: e0153979. 162 302 WB, EM
Shank3 is localized in axons and presynaptic specializations of developing hippocampal neurons and involved in the modulation of NMDA receptor levels at axon terminals.
Halbedl S, Schoen M, Feiler MS, Boeckers TM, Schmeisser MJ
Journal of neurochemistry (2016) 1371: 26-32. 162 302 WB, ICC
Reduced Glutamate Release in Adult BTBR Mouse Model of Autism Spectrum Disorder.
Wei H, Ma Y, Ding C, Jin G, Liu J, Chang Q, Hu F, Yu L
Neurochemical research (2016) 4111: 3129-3137. 162 302 WB, EM
Inhibition of IL-6 trans-signaling in the brain increases sociability in the BTBR mouse model of autism.
Wei H, Ma Y, Liu J, Ding C, Jin G, Wang Y, Hu F, Yu L
Biochimica et biophysica acta (2016) 186210: 1918-25. 162 302 WB, IHC; tested species: mouse
Effects of trace metal profiles characteristic for autism on synapses in cultured neurons.
Hagmeyer S, Mangus K, Boeckers TM, Grabrucker AM
Neural plasticity (2015) 2015: 985083. 162 302 ICC, WB
REST-Governed Gene Expression Profiling in a Neuronal Cell Model Reveals Novel Direct and Indirect Processes of Repression and Up-Regulation.
Garcia-Manteiga JM, Bonfiglio S, Folladori L, Malosio ML, Lazarevic D, Stupka E, Cittaro D, Meldolesi J
Frontiers in cellular neuroscience (2015) 9: 438. 162 302 WB
Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome.
Yoon S, Santos MD, Forrest MP, Pratt CP, Khalatyan N, Mohler PJ, Savas JN, Penzes P
Cell reports (2023) 427: 112784. 162 302 WB, IP; tested species: mouse
ZCCHC17 knockdown phenocopies Alzheimer's disease-related loss of synaptic proteins and hyperexcitability.
Cortese GP, Bartosch AMW, Xiao H, Gribkova Y, Lam TG, Argyrousi EK, Sivakumar S, Cardona C, Teich AF
Journal of neuropathology and experimental neurology (2024) : . 162 302 ICC; tested species: rat
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 302 WB, ICC, IHC; tested species: mouse
Autism-Associated Shank3 Is Essential for Homeostatic Compensation in Rodent V1.
Tatavarty V, Torrado Pacheco A, Groves Kuhnle C, Lin H, Koundinya P, Miska NJ, Hengen KB, Wagner FF, Van Hooser SD, Turrigiano GG
Neuron (2020) 1065: 769-777.e4. 162 302 ICC; tested species: mouse
The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons.
Lira M, Arancibia D, Orrego PR, Montenegro-Venegas C, Cruz Y, García J, Leal-Ortiz S, Godoy JA, Gundelfinger ED, Inestrosa NC, Garner CC, et al.
Molecular neurobiology (2018) : . 162 302 ICC; tested species: rat
Neuronal activity controls transsynaptic geometry.
Glebov OO, Cox S, Humphreys L, Burrone J
Scientific reports (2016) 6: 22703. 162 302 ICC
Shank3 is localized in axons and presynaptic specializations of developing hippocampal neurons and involved in the modulation of NMDA receptor levels at axon terminals.
Halbedl S, Schoen M, Feiler MS, Boeckers TM, Schmeisser MJ
Journal of neurochemistry (2016) 1371: 26-32. 162 302 WB, ICC
The effects of proteasomal inhibition on synaptic proteostasis.
Hakim V, Cohen LD, Zuchman R, Ziv T, Ziv NE
The EMBO journal (2016) 3520: 2238-2262. 162 302 ICC
Effects of trace metal profiles characteristic for autism on synapses in cultured neurons.
Hagmeyer S, Mangus K, Boeckers TM, Grabrucker AM
Neural plasticity (2015) 2015: 985083. 162 302 ICC, WB
Differential effectiveness of dietary zinc supplementation with autism-related behaviours in Shank2 knockout mice.
Lee K, Jung Y, Vyas Y, Mills Z, McNamara L, Montgomery JM
Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2024) 3791906: 20230230. 162 302 IHC; tested species: mouse
Dietary zinc supplementation rescues fear-based learning and synaptic function in the Tbr1+/- mouse model of autism spectrum disorders.
Lee K, Jung Y, Vyas Y, Skelton I, Abraham WC, Hsueh YP, Montgomery JM
Molecular autism (2022) 131: 13. 162 302 IHC; tested species: mouse
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 302 WB, ICC, IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 162 302 IHC; tested species: rat
Inhibition of IL-6 trans-signaling in the brain increases sociability in the BTBR mouse model of autism.
Wei H, Ma Y, Liu J, Ding C, Jin G, Wang Y, Hu F, Yu L
Biochimica et biophysica acta (2016) 186210: 1918-25. 162 302 WB, IHC; tested species: mouse
Modification of the synaptic cleft under excitatory conditions.
Tao-Cheng JH, Moreira SL, Winters CA, Reese TS, Dosemeci A
Frontiers in synaptic neuroscience (2023) 15: 1239098. 162 302 EM; tested species: mouse
Activity-dependent redistribution of CaMKII in the postsynaptic compartment of hippocampal neurons.
Tao-Cheng JH
Molecular brain (2020) 131: 53. 162 302 EM; tested species: rat
Neural precursor cells form integrated brain-like tissue when implanted into rat cerebrospinal fluid.
Pothayee N, Maric D, Sharer K, Tao-Cheng JH, Calac A, Bouraoud N, Pickel J, Dodd S, Koretsky A
Communications biology (2018) 1: 114. 162 302 EM; tested species: rat
Zinc Stabilizes Shank3 at the Postsynaptic Density of Hippocampal Synapses.
Tao-Cheng JH, Toy D, Winters CA, Reese TS, Dosemeci A
PloS one (2016) 115: e0153979. 162 302 WB, EM
Reduced Glutamate Release in Adult BTBR Mouse Model of Autism Spectrum Disorder.
Wei H, Ma Y, Ding C, Jin G, Liu J, Chang Q, Hu F, Yu L
Neurochemical research (2016) 4111: 3129-3137. 162 302 WB, EM
Cat. No.: 162 302
Amount: 200 µl
Price: $355.00
Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome.
Yoon S, Santos MD, Forrest MP, Pratt CP, Khalatyan N, Mohler PJ, Savas JN, Penzes P
Cell reports (2023) 427: 112784. 162 302 WB, IP; tested species: mouse
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 302 WB, ICC, IHC; tested species: mouse
SREBP2 gene therapy targeting striatal astrocytes ameliorates Huntington's disease phenotypes.
Birolini G, Verlengia G, Talpo F, Maniezzi C, Zentilin L, Giacca M, Conforti P, Cordiglieri C, Caccia C, Leoni V, Taroni F, et al.
Brain : a journal of neurology (2021) 14410: 3175-3190. 162 302 WB; tested species: mouse
Zinc Stabilizes Shank3 at the Postsynaptic Density of Hippocampal Synapses.
Tao-Cheng JH, Toy D, Winters CA, Reese TS, Dosemeci A
PloS one (2016) 115: e0153979. 162 302 WB, EM
Shank3 is localized in axons and presynaptic specializations of developing hippocampal neurons and involved in the modulation of NMDA receptor levels at axon terminals.
Halbedl S, Schoen M, Feiler MS, Boeckers TM, Schmeisser MJ
Journal of neurochemistry (2016) 1371: 26-32. 162 302 WB, ICC
Reduced Glutamate Release in Adult BTBR Mouse Model of Autism Spectrum Disorder.
Wei H, Ma Y, Ding C, Jin G, Liu J, Chang Q, Hu F, Yu L
Neurochemical research (2016) 4111: 3129-3137. 162 302 WB, EM
Inhibition of IL-6 trans-signaling in the brain increases sociability in the BTBR mouse model of autism.
Wei H, Ma Y, Liu J, Ding C, Jin G, Wang Y, Hu F, Yu L
Biochimica et biophysica acta (2016) 186210: 1918-25. 162 302 WB, IHC; tested species: mouse
Effects of trace metal profiles characteristic for autism on synapses in cultured neurons.
Hagmeyer S, Mangus K, Boeckers TM, Grabrucker AM
Neural plasticity (2015) 2015: 985083. 162 302 ICC, WB
REST-Governed Gene Expression Profiling in a Neuronal Cell Model Reveals Novel Direct and Indirect Processes of Repression and Up-Regulation.
Garcia-Manteiga JM, Bonfiglio S, Folladori L, Malosio ML, Lazarevic D, Stupka E, Cittaro D, Meldolesi J
Frontiers in cellular neuroscience (2015) 9: 438. 162 302 WB
Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome.
Yoon S, Santos MD, Forrest MP, Pratt CP, Khalatyan N, Mohler PJ, Savas JN, Penzes P
Cell reports (2023) 427: 112784. 162 302 WB, IP; tested species: mouse
ZCCHC17 knockdown phenocopies Alzheimer's disease-related loss of synaptic proteins and hyperexcitability.
Cortese GP, Bartosch AMW, Xiao H, Gribkova Y, Lam TG, Argyrousi EK, Sivakumar S, Cardona C, Teich AF
Journal of neuropathology and experimental neurology (2024) : . 162 302 ICC; tested species: rat
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 302 WB, ICC, IHC; tested species: mouse
Autism-Associated Shank3 Is Essential for Homeostatic Compensation in Rodent V1.
Tatavarty V, Torrado Pacheco A, Groves Kuhnle C, Lin H, Koundinya P, Miska NJ, Hengen KB, Wagner FF, Van Hooser SD, Turrigiano GG
Neuron (2020) 1065: 769-777.e4. 162 302 ICC; tested species: mouse
The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons.
Lira M, Arancibia D, Orrego PR, Montenegro-Venegas C, Cruz Y, García J, Leal-Ortiz S, Godoy JA, Gundelfinger ED, Inestrosa NC, Garner CC, et al.
Molecular neurobiology (2018) : . 162 302 ICC; tested species: rat
Neuronal activity controls transsynaptic geometry.
Glebov OO, Cox S, Humphreys L, Burrone J
Scientific reports (2016) 6: 22703. 162 302 ICC
Shank3 is localized in axons and presynaptic specializations of developing hippocampal neurons and involved in the modulation of NMDA receptor levels at axon terminals.
Halbedl S, Schoen M, Feiler MS, Boeckers TM, Schmeisser MJ
Journal of neurochemistry (2016) 1371: 26-32. 162 302 WB, ICC
The effects of proteasomal inhibition on synaptic proteostasis.
Hakim V, Cohen LD, Zuchman R, Ziv T, Ziv NE
The EMBO journal (2016) 3520: 2238-2262. 162 302 ICC
Effects of trace metal profiles characteristic for autism on synapses in cultured neurons.
Hagmeyer S, Mangus K, Boeckers TM, Grabrucker AM
Neural plasticity (2015) 2015: 985083. 162 302 ICC, WB
Differential effectiveness of dietary zinc supplementation with autism-related behaviours in Shank2 knockout mice.
Lee K, Jung Y, Vyas Y, Mills Z, McNamara L, Montgomery JM
Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2024) 3791906: 20230230. 162 302 IHC; tested species: mouse
Dietary zinc supplementation rescues fear-based learning and synaptic function in the Tbr1+/- mouse model of autism spectrum disorders.
Lee K, Jung Y, Vyas Y, Skelton I, Abraham WC, Hsueh YP, Montgomery JM
Molecular autism (2022) 131: 13. 162 302 IHC; tested species: mouse
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry.
Lutz AK, Bauer HF, Ioannidis V, Schön M, Boeckers TM
Frontiers in synaptic neuroscience (2022) 14: 890231. 162 302 WB, ICC, IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 162 302 IHC; tested species: rat
Inhibition of IL-6 trans-signaling in the brain increases sociability in the BTBR mouse model of autism.
Wei H, Ma Y, Liu J, Ding C, Jin G, Wang Y, Hu F, Yu L
Biochimica et biophysica acta (2016) 186210: 1918-25. 162 302 WB, IHC; tested species: mouse
Modification of the synaptic cleft under excitatory conditions.
Tao-Cheng JH, Moreira SL, Winters CA, Reese TS, Dosemeci A
Frontiers in synaptic neuroscience (2023) 15: 1239098. 162 302 EM; tested species: mouse
Activity-dependent redistribution of CaMKII in the postsynaptic compartment of hippocampal neurons.
Tao-Cheng JH
Molecular brain (2020) 131: 53. 162 302 EM; tested species: rat
Neural precursor cells form integrated brain-like tissue when implanted into rat cerebrospinal fluid.
Pothayee N, Maric D, Sharer K, Tao-Cheng JH, Calac A, Bouraoud N, Pickel J, Dodd S, Koretsky A
Communications biology (2018) 1: 114. 162 302 EM; tested species: rat
Zinc Stabilizes Shank3 at the Postsynaptic Density of Hippocampal Synapses.
Tao-Cheng JH, Toy D, Winters CA, Reese TS, Dosemeci A
PloS one (2016) 115: e0153979. 162 302 WB, EM
Reduced Glutamate Release in Adult BTBR Mouse Model of Autism Spectrum Disorder.
Wei H, Ma Y, Ding C, Jin G, Liu J, Chang Q, Hu F, Yu L
Neurochemical research (2016) 4111: 3129-3137. 162 302 WB, EM
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