Synaptic Systems

Cat. No.: 340 011

Amount: 100 µg

Price: $415.00

Cat. No. 340 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not recommended IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	64G6
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 77 from mouse SERT (UniProt Id: Q60857)
Reactivity	Reacts with: mouse (Q60857). No signal: human, rat. Other species not tested yet.
Data sheet	340_011.pdf

References for SERT - 340 011

Dysfunctional serotonergic neuron-astrocyte signaling in depressive-like states.
González-Arias C, Sánchez-Ruiz A, Esparza J, Sánchez-Puelles C, Arancibia L, Ramírez-Franco J, Gobbo D, Kirchhoff F, Perea G
Molecular psychiatry (2023) : . 340 011 IHC; tested species: mouse

Brain-derived neurotrophic factor expression in serotonergic neurons improves stress resilience and promotes adult hippocampal neurogenesis.
Leschik J, Gentile A, Cicek C, Péron S, Tevosian M, Beer A, Radyushkin K, Bludau A, Ebner K, Neumann I, Singewald N, et al.
Progress in neurobiology (2022) 217: 102333. 340 011 IHC; tested species: mouse

Comprehensive topographical map of the serotonergic fibers in the male mouse brain.
Awasthi JR, Tamada K, Overton ETN, Takumi T
The Journal of comparative neurology (2020) : . 340 011 IHC; tested species: mouse

Cat. No.: 340 011

Amount: 100 µg

Price: $415.00

IHC

IHC-P

Background

This serotonin transporter (SERT), also referred to as 5HTT and Slc6a4, is a monoamine transporter protein that transports serotonin back from the synaptic cleft into the presynaptic neuron to terminate the action of this neurotransmitter.

1 ) Montgomery TR et al., J. Neurosci. (2014)
2 ) Noristani HN et al., Cell Death Dis (2011)
3 ) Legutko R et al., Brain Res. (2001)
4 ) Najib A et al., FEBS Lett. (2000)
5 ) Fujita M et al., Neurosci. Lett. (1993)
6 ) Langer SZ et al., Experientia (1988)

Protocols

IHC

IHC-P

Other products for this target

SERT antibody - 340 011

References for SERT - 340 011