Synaptic Systems

Cat. No.: 340 003

Amount: 50 µg

Price: $375.00

Cat. No. 340 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 200 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 77 from mouse SERT (UniProt Id: Q60857)
Reactivity	Reacts with: rat (P31652), mouse (Q60857). Other species not tested yet.
Specificity	K.O. validated PubMed: 28362488
Data sheet	340_003.pdf

References for SERT - 340 003

Sex and brain region-specific regulation of serotonin transporter activity in synaptosomes in guanine nucleotide-binding protein G(q) alpha knockout mice.
Haase J, Jones AKC, Mc Veigh CJ, Brown E, Clarke G, Ahnert-Hilger G
Journal of neurochemistry (2021) : . 340 003 WB; tested species: mouse

TNFα-dependent anhedonia and upregulation of hippocampal serotonin transporter activity in a mouse model of collagen-induced arthritis.
Brown E, Mc Veigh CJ, Santos L, Gogarty M, Müller HK, Elfving B, Brayden DJ, Haase J
Neuropharmacology (2018) : . 340 003 WB; tested species: mouse

Altered dopamine release and monoamine transporters in Vps35 p.D620N knock-in mice.
Cataldi S, Follett J, Fox JD, Tatarnikov I, Kadgien C, Gustavsson EK, Khinda J, Milnerwood AJ, Farrer MJ
NPJ Parkinson's disease (2018) 4: 27. 340 003 WB; tested species: mouse

Serotonin Transporter Associated Protein Complexes Are Enriched in Synaptic Vesicle Proteins and Proteins Involved in Energy Metabolism and Ion Homeostasis.
Haase J, Grudzinska-Goebel J, Müller HK, Münster-Wandowski A, Chow E, Wynne K, Farsi Z, Zander JF, Ahnert-Hilger G
ACS chemical neuroscience (2017) 85: 1101-1116. 340 003 WB, EM; KO verified; tested species: mouse,rat

Fluoxetine reverses early-life stress-induced depressive-like behaviors and region-specific alterations of monoamine transporters in female mice.
Zheng JY, Li XX, Liu X, Zhang CC, Sun YX, Ma YN, Wang HL, Su YA, Si TM, Li JT
Pharmacology, biochemistry, and behavior (2024) 237: 173722. 340 003 IHC; tested species: mouse

Enoxaparin promotes functional recovery after spinal cord injury by antagonizing PTPRσ.
Ito S, Ozaki T, Morozumi M, Imagama S, Kadomatsu K, Sakamoto K
Experimental neurology (2021) 340: 113679. 340 003 IHC; tested species: rat

The development of synaptic transmission is time-locked to early social behaviors in rats.
Naskar S, Narducci R, Balzani E, Cwetsch AW, Tucci V, Cancedda L
Nature communications (2019) 101: 1195. 340 003 IHC; tested species: rat

Cat. No.: 340 003

Amount: 50 µg

Price: $375.00

ICC

IHC

IHC-P

Background

This serotonin transporter (SERT), also referred to as 5HTT and Slc6a4, is a monoamine transporter protein that transports serotonin back from the synaptic cleft into the presynaptic neuron to terminate the action of this neurotransmitter.

1 ) Montgomery TR et al., J. Neurosci. (2014)
2 ) Noristani HN et al., Cell Death Dis (2011)
3 ) Legutko R et al., Brain Res. (2001)
4 ) Najib A et al., FEBS Lett. (2000)
5 ) Fujita M et al., Neurosci. Lett. (1993)
6 ) Langer SZ et al., Experientia (1988)

Protocols

Standard protocol

ICC

IHC

IHC-P

Other products for this target

SERT antibody - 340 003 K.O.

References for SERT - 340 003