SecE antibody - 381 002

SecE is an integral membrane proteine involved in protein secretion by bacteria

Rabbit polyclonal antiserum

Cat. No.: 381 002

Amount: 200 µl

Price: $355.00

Cat. No. 381 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 144 from Mycobacterium smegmatis SecE
Reactivity	Reacts with: prokaryotes. Other species not tested yet.
Data sheet	381_002.pdf

Cat. No.: 381 002

Amount: 200 µl

Price: $355.00

Background

Bacteria have developed several systems for secreting their proteins to the extracellular space. Sec mediated export is necessary for the export of unfolded proteins to the extracytoplasmic environment. Central to the Sec pathway is a heterotrimeric protein complex composed of the SecY, SecE and SecG proteins which form a channel in the cytoplasmic membrane.
The cytoplasmic ATPase SecA is associated with the SecYEG complex and together they translocate proteins through the channel with repeated rounds of ATP-binding and hydrolysis.

1 ) Feltcher ME et al., Future Microbiol (2010)
2 ) Champion PA et al., Cell. Microbiol. (2007)
3 ) Nguyen L et al., Trends Cell Biol. (2005)
4 ) Braunstein M et al., J. Bacteriol. (2001)

Protocols