SAP97 antibody - 124 315

SAP97 is a postsynaptic PDZ-domain protein of the MAGUK family

Guinea pig polyclonal purified antibody

Cat. No.: 124 315

Amount: 50 µg

Price: $460.00

Cat. No. 124 315	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not recommended IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 130 from rat SAP97 (UniProt Id: Q62696)
Reactivity	Reacts with: rat (Q62696), mouse (Q811D0). Other species not tested yet.
Specificity	Shows band of expected size in westernblots.
Data sheet	124_315.pdf

Cat. No.: 124 315

Amount: 50 µg

Price: $460.00

Background

SAP 97 (synapse associated protein of 97 kDa, also called DLG 1) like PSD 95 and SAP 102, is a scaffolding protein of the postsynaptic density. It consists of three type-one PDZ domains and binds selectively to the C-terminus of the GluR1 subunit of AMPA receptors.
SAP 97 is probably involved in the clustering of AMPA receptors at the post-synaptic membrane.
It is also expressed in heart, where it has been shown to be involved in the targeting of voltage gated potassium channels to subpopulations of lipid rafts.

1 ) Cai C et al., J. Biol. Chem. (2006)
2 ) Folco EJ et al., Am. J. Physiol. Heart Circ. Physiol. (2004)
3 ) Leonoudakis D et al., J. Biol. Chem. (2004)
4 ) Gardoni F et al., J. Biol. Chem. (2003)
5 ) Mehta S et al., J. Biol. Chem. (2001)
6 ) Valtschanoff JG et al., Eur. J. Neurosci. (2000)
7 ) Tiffany AM et al., J. Cell Biol. (2000)
8 ) Bassand P et al., Eur. J. Neurosci. (1999)
9 ) Leonard AS et al., J. Biol. Chem. (1998)

Protocols

Standard protocol

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